SARS-CoV-2 Nucleic Acid Detection Kit (Multiplex PCR Fluorescent Probe Method)
Tšebeliso e reriloeng
Setsi sena se sebelisa theknoloji ea Real-time RT PCR (rRT-PCR) bakeng sa ho lemoha boleng ba SARS-CoV-2 nucleic acid lisampoleng tsa motho tsa nasopharyngeal kapa oropharyngeal swab.
Molao-motheo oa teko
Sets'oants'o sena sa li-primer le probe se etselitsoe ho bona tatellano e bolokiloeng ea SARS-CoV-2 (ORF1ab gene le N gene).Thepa e na le Taolo ea ka Hare e sebelisetsoang ho lekola boleng ba mohlala.
Theknoloji ea nako ea 'nete ea PCR e sebelisa polymerase chain reaction (PCR) bakeng sa ho holisa sepheo se itseng.
tatellano le ho shebana le li-probes tse khethehileng bakeng sa ho lemoha RNA e matlafalitsoeng.Li-probe li ngotsoe ka
moqolotsi oa litaba oa fluorescent le lidae tsa quencher.
Lintlha tse Potlakileng
◮Litlhaloso:48 Rxns/kit, 96 Rxns/kit
◮ Tsebo:96.72%
Kutloisiso:97.92%
◮ Mefuta ea mehlala e sebetsang
Nasopharyngeal Swab kapa Oropharyngeal Swab
◮Sesebelisoa se sebetsang
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Likaroloana
Che. | Karolo | Chelete | Likarolo tse ka sehloohong | |||
48 rxn | 96 Rxns | |||||
1 | Moemeli oa ho lokolloa ha Nucleic acid | 1.4mL / tube | 2 li-tubes | 5.3 mL/botlolo | 1 botlolo | Surfactant |
2 | Tšireletso ea RNA | 27 μL / tube | 1 tube | 53 μL / tube | 1 tube | RNase inhibitor |
3 | Tharollo ea karabelo ea SARS-CoV-2 | 800 μL / tube | 1 tube | 1600 μL / tube | 1 tube | Primer, probe, buffer reaction, dNTP |
4 | Motsoako oa enzyme ea SARS-CoV-2 | 80 μL / tube | 1 tube | 160 μL / tube | 1 tube | Ho qala enzyme ea Taq, enzyme ea M-MLV |
5 | SARS-CoV-2 taolo e ntle | 100 μL / tube | 1 tube | 100 μL / tube | 1 tube | Recombinant plasmid e nang le sekhechana sa sepheo, RNA |
6 | SARS-CoV-2 taolo e mpe | 1200 μL / tube | 1 tube | 1200 μL / tube | 1 tube | TE buffer |
Likarolo le melemo
◮PCR e tobileng
Ha ho na tlhoko ea "nucleic acid extaction kit" le "System" e phethiloeng ka mohlala oa 96 ka hora e le 'ngoe kapa ho feta.
◮Tlhokahalo e tlase ea lisebelisoa le tšebeliso e feto-fetohang haholo
E hloka feela sistimi ea 'nete ea nako ea PCR.
◮LoD le kutloisiso e phahameng
Boikutlo ba ho lemoha bo ka fihla boemong bo tlaase ka ho fetisisa joaloka likopi tse 500 / ml.
Mokhoa oa ho sebelisa
Mokhoa oa 1: Mokhoa o tobileng oa PCR
Mokhoa oa 2: Viral RNA Isolation + PCR
Mokhoa oa 1: Mokhoa oa PCR o tobileng o sebelisaMoemeli oa Phallo ea Mohlala
Lipalangoang le Polokelo ea Cold Chain
E tiisitsoe khanyeng 'me e bolokiloe ho -20±5℃;
Shelf bophelo
1 selemo
Ho Odara Boitsebiso
Video ea ts'ebetso e potlakileng
Ngola molaetsa wa hao mona mme o re romele wona