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Lisele tsa liphoofolo Direct PCR kit-UNG
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho lokolla DNA ea genomic kapele ho mehlala ea lisele tsa liphoofolo bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa liphatsa tsa lefutso tse kholo.
Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65°C. Ha ho hlokahale lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA, 'me mohlala oa DNA o lokollotsoeng o ka sebelisoa e le template ea karabelo ea PCR.
2×PCR BonoloTM Mix (UNG) e sebelisa dUTP ho fapana le dTTP motheong oa 2×PCR BonoloTM Kopanya, mme o eketsa enzyme ea UNG (Uracil-N-glycosylase) e ka nyelisang template e nang le DUTP ka nako e le ngoe. Pele karabelo ea PCR, enzyme ea UNG e sebelisoa ho nyenyefatsa sehlahisoa sa PCR se nang le uracil. Enzyme ea UNG e ke ke ea ba le phello ho template e se nang uracil, ka hona e netefatsa ho nepahala le ho nepahala ha matlafatso le ho thibela monyetla oa lihlahisoa tsa PCR tse silafetseng nakong ea liteko tse kholo tsa lefutso.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
