China Animal Tissue DNA Isolation Kit DNA Extraction Kits from Animal Tissue Samples Moetsi le Mofani |Foregene

Animal Tissue DNA Isolation Kit DNA Extraction Kits from Animal Tissue Samples

Tlhaloso ea Kit:

Ho ntšoa ka potlako le tlhoekiso ea DNA ea genomic ho tsoa mehloling e mengata, joalo ka lisele tsa liphoofolo, lisele, joalo-joalo.

◮Lebelo le potlakileng:ts'ebetso e phahameng ho feta li-protease tse tšoanang le ho sila lisampole tsa linama kapele;

◮E bonolo:e ka phethoa ka metsotso e 50.

◮E loketse:E etsoa ka mocheso oa kamore.

◮Tšireletseho:ha ho na reagent ea organic e sebelisoang.

Re romelle lengolo-tsoibila Khoasolla e le PDF

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso tsa Kit

Setsi sena se sebelisa kholomo ea DNA-feela e ka kopanyang DNA, Foregene protease le sistimi e ikhethang ea buffer.DNA ea boleng bo holimo e ka ntšoa liseleng tse fapaneng tsa setso le liseleng tsa liphoofolo nakong ea metsotso e 30 ho isa ho e 50.

The DNA-feela silica gel lera sebelisoa ka ho spin kholomo ke Foregene e ncha e ikhethang lintho tse bonahalang, e ka ka tsela e atlehileng le ka ho toba tlama ho DNA, 'me ka ho fetisisa ho tloswa ha RNA, litšila liprotheine, ion le metsoako e meng ea manyolo ka liseleng.5-80μg DNA ea boleng bo phahameng ea genomic e ka hloekisoa ho tloha ho 10-50mg tissue.

Litlhaloso

DNA ea Lithane tsa Phoofolo Isolation Kit
Likahare tsa Kit	DE-05011	DE-05012	DE-05013
Likahare tsa Kit	50T	100T	250T
Buffer L1	20ml	40ml	100ml
Buffer L2*	20ml	40ml	100ml
Buffer PW*	25ml	50ml	125ml
Buffer WB	25ml	50ml	125ml
Buffer EB	10ml	20ml	50ml
Foregene Protease	1.25ml	2.5ml	6.5ml
DNA-Feela Kholomo	50	100	250
Bukana	1	1	1

*: Buffer L2 le Buffer PW li na le letsoai le hloekisitsoeng le hlabang.Ka kopo, roala liatlana 'me u nke mehato e nepahetseng ea ts'ireletso ha u sebetsa.

Likarolo le melemo

■ Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ka har'a kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le RNase e eketsehileng nakong ea teko, kahoo e thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.

■ Lebelo le potlakileng: Foregene Protease e na le tšebetso e phahameng ho feta ea protease e tšoanang ’me e sila lisampole tsa linama kapele.

■ E bonolo : ts'ebetso ea ho ntša DNA ea genomic e ka phethoa ka metsotso e 50.

■ Bonolo: The centrifugation e etsoa mocheso oa kamore, ha ho 4℃ mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA e hlokahalang.

■ Tšireletseho: ha ho na reagent ea tlhaho e hlokahalang.

■ Boleng bo phahameng: DNA e nkiloeng ea genomic e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlaase haholo, li ka finyella litlhoko tsa liteko tse fapa-fapaneng.