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Lisele tsa liphoofolo tsa PCR Direct
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho lokolla DNA ea genomic kapele ho mehlala ea lisele tsa liphoofolo bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa liphatsa tsa lefutso tse kholo.
Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65°C. Ha ho hlokahale lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA, 'me mohlala oa DNA o lokollotsoeng o ka sebelisoa e le template ea karabelo ea PCR.
2× PCR BonoloTM Motsoako o na le mamello e matla ho li-inhibitor tsa PCR, 'me o ka sebelisa lysate ea sampole e tla lekoa e le template ea ntlafatso e sebetsang le e ikhethang. Reagent ena e na le Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, buffer ea reaction, PCR optimizer le stabilizer.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
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Mouse Mohatla SuperDirect PCR Kit
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho lokolla DNA ea genomic kapele ho tsoa mohatleng oa toeba le litsebeng tsa litsebe bakeng sa karabelo ea PCR, ka hona e loketse liteko tsa lefutso tse kholo.
Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe pele ho metsotso e 10-30 mochesong oa kamore (20-25 ° C). Ha ho hlokahale ho futhumatsa, mme lits'ebetso tse ling tse kang ho tlosa protheine le RNA ha li hlokehe. Palo ea DNA e lokollotsoeng e ka sebelisoa e le Setšoantšo sa karabelo ea PCR.
2 × M-PCR Easy TM Mix e na le mamello e matla ho li-inhibitor tsa PCR, 'me e ka sebelisa lysate ea sampole e tla hlahlojoa e le template bakeng sa kholiso e sebetsang hantle le e ikhethang. Reagent ena e na le Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer le stabilizer.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
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Lisele tsa liphoofolo Direct PCR kit-UNG
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho lokolla DNA ea genomic kapele ho mehlala ea lisele tsa liphoofolo bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa liphatsa tsa lefutso tse kholo.
Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65°C. Ha ho hlokahale lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA, 'me mohlala oa DNA o lokollotsoeng o ka sebelisoa e le template ea karabelo ea PCR.
2×PCR BonoloTM Mix (UNG) e sebelisa dUTP ho fapana le dTTP motheong oa 2×PCR BonoloTM Kopanya, mme o eketsa enzyme ea UNG (Uracil-N-glycosylase) e ka nyelisang template e nang le DUTP ka nako e le ngoe. Pele karabelo ea PCR, enzyme ea UNG e sebelisoa ho nyenyefatsa sehlahisoa sa PCR se nang le uracil. Enzyme ea UNG e ke ke ea ba le phello ho template e se nang uracil, ka hona e netefatsa ho nepahala le ho nepahala ha matlafatso le ho thibela monyetla oa lihlahisoa tsa PCR tse silafetseng nakong ea liteko tse kholo tsa lefutso.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
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Mouse Mohatla Direct PCR Kit
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho lokolla kapele genomic DNA ho tsoa mehatleng ea toeba, litsebe, mesifa le mehlala e meng ea lisele bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa lefutso tse kholo.
Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65 ° C. Ha ho lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA tse hlokahalang, 'me DNA e lokollotsoeng e ka sebelisoa e le template ea karabelo ea PCR.
2 × M1-PCR Easy TM Mix e na le mamello e matla ho li-inhibitor tsa PCR, 'me e ka sebelisa lysate ea sampole e tla hlahlojoa e le template ea ntlafatso e sebetsang le e ikhethang. Reagent ena e na le Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer le stabilizer.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
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Mouse Mohatla Direct PCR Kit-UNG
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho lokolla kapele genomic DNA ho tsoa mehatleng ea toeba, litsebe, mesifa le mehlala e meng ea lisele bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa lefutso tse kholo.
Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65 ° C. Ha ho lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA tse hlokahalang, 'me DNA e lokollotsoeng e ka sebelisoa e le template ea karabelo ea PCR.
2 × M1-PCR Easy TM Mix (UNG) e sebelisa DUTP sebakeng sa dTTP motheong oa 2 × M1-PCR Easy TM Mix (UNG), mme e eketsa enzyme ea UNG (Uracil-N-glycosylase) e ka nyelisang template e nang le DUTP ho ka nako e ts'oanang. Pele karabelo ea PCR, enzyme ea UNG e sebelisoa ho nyenyefatsa sehlahisoa sa PCR se nang le uracil. Enzyme ea UNG e ke ke ea ba le phello ho template e se nang uracil, ka tsela eo e netefatsa bonnete le ho nepahala ha matlafatso le ho thibela monyetla oa liteko tse kholo tsa liphatsa tsa lefutso. Tšilafalo ea lihlahisoa tsa PCR e etsahetse.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
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Zebra Fish Direct PCR Kit
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho tsoa kapele DNA ea genomic ho tsoa ho li-zebrafish le lisele tse ling tsa tlhapi ea metsi a hloekileng, mapheoana a mohatla kapa mehlala ea mahe a tlhapi bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa lefutso tse kholo. Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65 ° C. Ha ho lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA tse hlokahalang, 'me DNA e lokollotsoeng e ka sebelisoa e le template ea karabelo ea PCR.
2 × PCR Easy TM Mix e na le mamello e matla ho li-inhibitor tsa PCR, 'me e ka sebelisa lysate ea sampole e tla hlahlojoa e le template bakeng sa kholiso e sebetsang hantle le e ikhethang. Reagent ena e na le ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer le stabilizer. E sebelisoa hammoho le buffer ea lysis e ka lemoha mehlala ka potlako le ka mokhoa o bonolo, 'me e na le litšobotsi tsa kutloisiso e phahameng, ho khetheha ho matla le botsitso bo botle.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
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Zebra Fish Ka ho toba PCR Kit-UNG
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho tsoa kapele DNA ea genomic ho tsoa ho li-zebrafish le lisele tse ling tsa tlhapi ea metsi a hloekileng, mapheoana a mohatla kapa mehlala ea mahe a tlhapi bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa lefutso tse kholo. Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65 ° C. Ha ho lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA tse hlokahalang, 'me DNA e lokollotsoeng e ka sebelisoa e le template ea karabelo ea PCR.
2 × PCR Easy TM Mix (UNG) e sebelisa DUTP sebakeng sa dTTP motheong oa 2 × PCR Easy TM Mix, mme e eketsa enzyme ea UNG (Uracil-N-glycosylase) e ka nyelisang template e nang le DUTP ka nako e le ngoe. Pele karabelo ea PCR, enzyme ea UNG e sebelisoa ho nyenyefatsa sehlahisoa sa PCR se nang le uracil. Enzyme ea UNG e ke ke ea ba le phello ho template e se nang uracil, ka tsela eo e netefatsa bonnete le ho nepahala ha matlafatso le ho thibela monyetla oa liteko tse kholo tsa liphatsa tsa lefutso. Tšilafalo ea lihlahisoa tsa PCR e etsahetse.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
