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Semela sa DNA Isolation Kit Genomic Plant DNA Purification Kits Reagents Protocol
Cat.No.DE-06111/06112/06113
Bakeng sa tlhoekiso ea DNA ea genomic ho tsoa ho lisele tse fapaneng tsa semela.
Ka potlako hloekisa 'me u fumane DNA ea boleng bo holimo ho tloha lisampoleng tsa limela (ho kenyeletsoa li-polysaccharides le disampole tsa limela tsa polyphenol).
◮Ha ho tšilafalo ea RNase
◮Lebelo le potlakileng
◮E bonolo: Ts'ebetso ea tlhoekiso e ka phethoa ka metsotso e 30.
◮E loketseng: Mocheso oa kamore, 4℃ centrifugation le ethanol precipitation ea DNA ha e hlokehe.
◮Polokeho: ha ho na reagent ea tlhaho e sebelisoang.
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Animal Tissue DNA Isolation Kit DNA Extraction Kits from Animal Tissue Samples
Ho ntšoa ka potlako le tlhoekiso ea DNA ea genomic ho tsoa mehloling e mengata, joalo ka lisele tsa liphoofolo, lisele, joalo-joalo.
◮Lebelo le potlakileng:ts'ebetso e phahameng ho feta li-protease tse tšoanang le ho sila lisampole tsa linama kapele;
◮E bonolo:e ka phethoa ka metsotso e 50.
◮E loketse:E etsoa ka mocheso oa kamore.
◮Tšireletseho:ha ho na reagent ea organic e sebelisoang.
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Mali a DNA Mini Kit
Ka potlako hloekisa DNA ea boleng bo phahameng ba genomic maling a anticoagulated (<1ml).
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Kakaretso ea Plasmid Mini Kit Kakaretso ea Plasmid DNA Extraction Miniprep Mini Kits
Ka potlako hloekisa plasmid DNA ea boleng bo holimo ho tsoa ho libaktheria tse fetotsoeng bakeng sa liteko tse tloaelehileng tsa baeloji ea limolek'hule joalo ka phetoho le tšilo ea enzyme.
◮Tlosa RNA ntle le ho eketsa RNase
◮E loketseng- centrifugation e etsoa ka mocheso oa kamore
◮Ka potlako- ts'ebetso e ka phethoa ka metsotso e 20
◮Sireletsehile- ha ho na reagent ea tlhaho e sebelisoang
◮Bohloeki bo phahameng—OD260/280≈1.7-1.9
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Mali a DNA Midi Kit (1-5ml) Mali a Isolation Midi Kits for DNA from Blood
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa maling a anticoagulated (1-5ml).
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction or Purifiction Kit from Buccal Swabs
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa lisampoleng tsa buccal swab/FTA Card.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Genomic DNA Micro Kit Micro Genomic DNA Mini Kit
Ka potlako hloekisa lisampole tse nyane ho fumana DNA ea boleng bo holimo.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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DNA Isolation Kit ea mahala ea DNA
Ho aroloa ha cfDNA ho lisampole tsa plasmaNomoro ea Lethathamo la TQ01BT0050, TQ01BT0100 -
Metsi DNA Isolation Kit DNA Extraction le Purification Kit bakeng sa Metsi
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho likokoana-hloko ka lisampole tse fapaneng tsa metsi.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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Stool DNA Isolation Kit DNA Extraction Purification Kits from Stool
Ka potlako hloekisa 'me u fumane DNA ea boleng bo holimo ho tsoa lisampoleng tse fapaneng tsa mantle.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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PCR Purification Kit PCR Clean-up System Bakeng sa Ultra-pure DNA
Ka potlako hloekisa le ho fumana likhechana tsa DNA tsa boleng bo holimo ho tsoa ho sistimi ea PCR;haeba u batla ho arola le ho fumana likhechana tse khethehileng tsa DNA, ka kopo khetha lisebelisoa tsa ho khutlisa li-gel.
◮ Mefuta e mengata ea ho hlaphoheloa ha DNA: Likaroloana tsa DNA tse khutšoane joalo ka 60bp le tse kholo joalo ka 10kb li ka fumanoa.
◮ Ts'ebetso e phahameng ea ho hlaphoheloa: Ts'ebetso ea ho hlaphoheloa hangata e feta 80%, 'me e phahameng ka ho fetisisa e ka fihla ho feta 95%.
◮ Lebelo le potlakileng:Ho bonolo ho sebetsa, ho hlaphoheloa ha sekhechana sa DNA ho ka phethoa nakong ea metsotso e 15 mme primer dimer e ka tlosoa ka kotloloho ntle le ho hlaphoheloa ha gel.
◮ Safety: Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮ Boleng bo phahameng: Likaroloana tse fumanoeng tsa DNA li hloekile haholo, tse ka kopanang le liteko tse fapaneng tse latelang.
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FFPE DNA Isolation Kit DNA Extraction Purification Kit ho tloha FFPE Samples
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa ho lithane tse kentsoeng ke parafun tse kang likarolo tsa parafun le liboloko tsa parafun.
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
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