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Ka ho toba RT-qPCR Kit II
◮Direct RT-qPCR Kit, e sebelisa Foreasy Reverse Transcriptase le Foreasy HS Taq DNA Polymerase e hlahisoang ke Foregene, e nang le buffer e ikhethang ea karabelo, e etsa hore kit ena e be le khanyetso e matla le e tsamaellanang, 'me e ka leka ka kotloloho ts'ebeliso ea sistimi ea Foregene Lysis joalo ka template.Setsi sena se ka etsa kopo bakeng sa lisebelisoa tsa ho bona tsa COVID-19 nucleic acid le nts'etsopele ea lisebelisoa tsa ho lemoha libaktheria tsa pathogenic tsa nucleic acid.
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Ka ho toba RT-qPCR Kit III
◮Direct RT-qPCR Kit, e sebelisa Foreasy Reverse Transcriptase le Foreasy HS Taq DNA Polymerase e hlahisoang ke Foregene, e nang le buffer e ikhethang ea karabelo, e etsa hore kit ena e be le khanyetso e matla le e tsamaellanang, 'me e ka leka ka kotloloho ts'ebeliso ea sistimi ea Foregene Lysis joalo ka template.Setsi sena se ka etsa kopo bakeng sa lisebelisoa tsa ho bona tsa COVID-19 nucleic acid le nts'etsopele ea lisebelisoa tsa ho lemoha libaktheria tsa pathogenic tsa nucleic acid.
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RT-qPCR Kit e tobileng
◮ Direct RT-qPCR Kit, e sebelisa Foreasy Reverse Transcriptase le Foreasy HS Taq DNA Polymerase e hlahisoang ke Foregene, e nang le buffer e ikhethang ea karabelo, e etsa hore kit ena e be le khanyetso e matla le e tsamaellanang, 'me e ka leka ka kotloloho ts'ebeliso ea sistimi ea Foregene Lysis joalo ka template.Setsi sena se ka etsa kopo bakeng sa lisebelisoa tsa ho bona tsa COVID-19 nucleic acid le nts'etsopele ea lisebelisoa tsa ho lemoha libaktheria tsa pathogenic tsa nucleic acid.
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Moemeli oa Phallo ea Mohlala
◮Ho bonolo ho sebetsa:mohlala oa lysis ha o ntse o bokella mohlala
◮E potlakile ebile e bonolo:Ha ho na tlhoko ea ho ntšoa ha nucleic acid, e loketseng ho fumanoa ka tekanyo e kholo
◮Sekoahelo se pharaletseng sa ts'ebeliso:PCR ka ho toba ka mor'a ho bokella sampole
◮E bolokehile ebile ea tšepahala:Fast inactivate kokoana-hloko, netefatsa polokeho ho isa tekanyong e kholo
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ForeDirect RT-qPCR Kit
◮Bakeng sa ntlafatso ea nako ea 'nete ea RNA ka kotloloho ho tsoa ho likoleke tsa swab ntle le lits'ebetso tsa tlhoekiso ea RNA.Sesebelisoa sena se phethela lipotoloho tsa qRT-PCR nakong ea hora e le 'ngoe.Motsoako oa enzyme ke motsoako o ntlafalitsoeng oa Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Reaction Buffer e na le likarolo tsohle tse hlokahalang, ho kenyeletsoa likarolo tsa buffer tse ntlafalitsoeng, Mg2+, dUTP, le dNTPs.
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E fumaneha habonolo M-MLV Reverse Transcriptase
Foreasy M-MLV Reverse Transcriptase ke reverse transcriptase e ncha e hlahisoang ke libaktheria tsa E. coli tse sebelisang theknoloji ea ho kopanya liphatsa tsa lefutso.Ke DNA polymerase e kopantsoeng hape e kopanyang DNA e tlatsetsang ho tloha ho RNA, DNA, kapa RNA: DNA ea mofuta o le mong.Ha e na ts'ebetso ea RNase H, botsitso bo matla, kamano e matla ea RNA, le kutloisiso e phahameng ea ho lemoha.
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Ho bonolo RNase Inhibitor
◮RNase inhibitor e ncha e entsoeng ke mouse e hlahisoang ke libaktheria tsa E. coli tse sebelisang theknoloji ea ho kopanya liphatsa tsa lefutso.Inhibitor e thibela ts'ebetso ea RNase ka ho se kopane le RNase 1: 1 ntle le tlholisano, kahoo e sireletsa.RNAbotšepehi , 'me e ka thibela ka katlehotheRNase A, B, C mosebetsi , empa eseng RNase T1, T2, H, joalo-joalo; E khutlisetsoa morao ea tlamo ea RNase Inhibitor le RNase , 'me e rarahaneng e ka aroloa ke urea le sulfhydryl reagents, e le hore RNase renatures le inhibitor e ke ke ea hlola e e-ba teng.
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HS Taq DNA Polymerase
◮Boemo bo phahameng: Enzyme e nang le ts'ebetso e phahameng ea ho qala ho chesa.
◮Phatlalatso e potlakileng: 10 sec/kb.
◮Boemo bo phahameng ba template : e ka sebelisoa ho holisa Phahameng ka nepoGCbolenglemefuta e fapaneng e thata ho holisa template ea DNA.
◮Botšepehi bo matla: Botšepehi ke makhetlo a 6of E tloaelehileng ea Taq Enzyme.
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Taq DNA Polymerase e bonolo
◮Boemo bo phahameng: Enzyme e na le ts'ebetso e itseng ea ho qala ho chesa.
◮Phatlalatso e potlakileng: 10 sec/kb.
◮Template e feto-fetohang haholo: e ka sebelisoa ho holisa boleng bo holimo ba GC, tse fapaneng tse thata ho holisa template ea DNA.
◮Botšepehi bo matla: Taq Enzyme e tloaelehileng makhetlo a 6.
◮Botsitso bo matla ba mocheso: E ka behoa ho 37 ° C ka beke 'me e boloka ts'ebetso e fetang 90%.
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