Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Mohatla)(bakeng sa Genotyping)
Litlhaloso
Setsi sena se sebelisa mokhoa o ikhethileng oa lysis buffer system ho lokolla ka potlako DNA ea genomic ho tloha mohatleng oa toeba le lisampole tsa tsebe ea toeba bakeng sa karabelo ea PCR, ka hona e loketse haholo tlhahlobo e kholo ea liphatsa tsa lefutso.
Mokhoa oa ho lokolla DNA ea genomic ho tswa ho lysis buffer e phethetsoenakong ea metsotso e 10-30homocheso oa kamore (20-25).°C).Ha ho hlokahale ho futhumatsa, 'me mekhoa e meng e kang ho tlosoa ha protheine le RNA ha e hlokehe.Lipalo tse lokollotsoeng tsa DNA li ka sebelisoa e le Template bakeng sa karabelo ea PCR.
2×M-PCR e BonoloTM Mix e na le mamello e matla ho PCR reaction inhibitors, 'me e ka sebelisa lysate ea sampole ho lekoa e le template bakeng sa ho holisa ka nepo le ka nepo.Reagent ena e na le Foregene D-Taq DNAPolymerase, dNTPs, MgCl.2, buffer ea karabelo, PCR optimizer le stabilizer.
D-Taq DNA polymerase ke DNA polymerase e entsoeng ka ho khetheha ke Foregene bakeng sa karabelo e tobileng ea PCR.D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho holisa DNA ka mokhoa o nepahetseng lits'ebetsong tse fapaneng tse rarahaneng tsa karabelo, mme lebelo la amplification le ka fihla ho 2Kb/min, e loketseng ka ho khetheha bakeng sa karabelo ea Direct PCR.
Litlhaloso
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Likarolo tsa Kit
Karolo ea I | Sekhahla sa NP |
Foregene Protease Plus I | |
6× DNA Loading Buffer | |
Karolo ea II | 2× M PCR EasyTM Mix |
Litaelo |
Likarolo le melemo
■Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.
■ Tlhokahalo ea mohlala e nyane, nka feela mehatla ea litoeba (kapa litsebe tsa litoeba tse bophara ba 2-5mm) ka holimo ho 2mm.
■ Ho bonolo ho sebetsa, e ka lysed ntle le ho futhumatsa ho etsa hore protease e sebetse, 'me sehlahisoa sa lysed se ka kenngoa ka ho toba ho PCR reaction.
■ Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) e ka sebelisoa ho ntša DNA ea genomic mohatleng o sebetsitsoeng oa likhoto.
■ Sistimi e ntlafalitsoeng ea PCR e etsa hore PCR e be le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
Litekanyetso tsa kit
Kopo: Mohatla oa mouse, litsebe tsa mouse, mesifa le lisele tse ling.
DNA e lokollotsoeng ho tsoa ho sampole lysis: e sebelisoa feela joalo ka template ea PCR.
Thepa e ka sebelisoa bakeng sa merero e latelang: ho tsebahatsa transgenosis, genotyping ea liphoofolo, joalo-joalo.
Phallo ea mosebetsi
Storage le Shelf bophelo
Karolo ea I ea kit ena e lokela ho bolokoa ho 2-8 ℃.
Reagent Buffer FP e ka bolokoa likhoeli tse 12 tlas'a maemo a omileng;e ka bolokoa ho 2-8 ℃ bakeng sa polokelo e telele.
Reagent Foregene Protease e na le foromo e ikhethang.Ho netefatsa ts'ebetso ea eona le botsitso, ka kopo e boloke ho 4 ℃ bakeng sa likhoeli tse 12.
Reagent 6×DNA Loading Buffer e ka bolokoa ho 4℃ kapa -20℃ nako e telele.
Karolo ea II ea kit ena e lokela ho bolokoa ho -20 ℃.
Reagent 2 × PCR EasyTM Mix, haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa ho fihlela matsatsing a 10).