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Ke phapang efe lipakeng tsa PCR, RT-PCR, qPCR, le RT-qPCR
PCR ke mokhoa o sebelisoang ho holisa DNA ho tsoa palo e nyane ea template ea DNA.RT-PCR e sebelisa transcript ho hlahisa template ea DNA ho tsoa mohloling oa RNA o ka holisoang.PCR le RT-PCR hangata ke karabelo ea pheletso, ha qPCR le RT-qPCR li sebelisa kinetics ea sekhahla sa tlhahiso ea lihlahisoa...Bala haholoanyane -
Ntho e ka Pele ea Tšebeliso ea Lithethefatsi: Ho lebisa RNA ka Limolek'hule tse Nyenyane
Ente ea Pfizer's mRNA bakeng sa COVID e busitse tjantjello ea ho sebelisa ribonucleic acid (RNA) joalo ka sepheo sa kalafo.Leha ho le joalo, ho lebisa tlhokomelo ea RNA ka limolek'hule tse nyenyane ho thata haholo.RNA e na le li-blocks tse 'ne feela: adenine (A), cytosine (C), guanine (G), le uracil (U) e nkang sebaka sa thymin ...Bala haholoanyane -
Lithethefatsi tsa nucleic acid ke eng?
“Nucleic acid drugs” e sebelisa “nucleic acid,” e bolelang lintho tse kang DNA le RNA tse laolang boitsebiso ba liphatsa tsa lefutso, e le lithethefatsi.Tsena li lumella ho shebisisa limolek'hule tse kang mRNA le miRNA tse ke keng tsa tobisoa ka lithethefatsi tse tloaelehileng tsa boima ba limolek'hule le lithethefatsi tsa antibody, 'me ho na le ...Bala haholoanyane -
Boiphihlelo bo bong ka siRNA ho RT-PCR
1. Tsebo ea motheo (haeba u batla ho bona karolo ea liteko, ka kopo fetisetsa ka ho toba karolong ea bobeli) E le karabelo e tsoang ho PCR e tloaelehileng, Nako ea sebele PCR e shebella haholo phetoho ea palo ea sehlahisoa sa amplification potolohong e 'ngoe le e' ngoe ea PCR amplification. karabelo ka nako ea nnete thr...Bala haholoanyane -
Kakaretso e qaqileng ea mokhoa oa RT-PCR karabelo ea sistimi ea liteko
一、Eketsa kutlo ea sistimi ea karabelo: 1. Arola RNA ea boleng bo holimo: Ts'ebetso ea cDNA e atlehileng e tsoa ho RNA ea boleng bo holimo.RNA ea boleng bo holimo bonyane e lokela ho ba bolelele bo felletseng 'me e se na li-reverse transcriptase inhibitors tse kang EDTA kapa SDS.Boleng ba RNA bo lekanya boholo ba ...Bala haholoanyane -
Tlatsa moralo oa mantlha oa PCR le lintlha tsa PCR
Primer design foundation (99% mathata a ka rarolloa) 1. Primer length: Buka ea thuto e hloka 15-30bp, hangata e ka bang 20bp.Boemo ba sebele bo molemo ho ba 18-24bp ho netefatsa ho khetheha, empa nako e telele e ntle, e telele haholo e tla fokotsa ho khetheha, le ho fokotsa chai.2. Prim...Bala haholoanyane -
Litlhokomelo bakeng sa qRT-PCR e hlophisitsoeng ke Ngaka e kholo ka hloko
Motho e mong le e mong o bua ka molao-motheo oa teko ea qRT-PCR, moralo oa pele, tlhaloso ea sephetho, joalo-joalo, empa ke nahana hore ke lokela ho arolelana le uena ts'ebetso ea liteko ea qRT-PCR.E nyane, empa e mabapi le liphetho.Pele re etsa qRT-PCR, re hloka ho ba le kutloisiso e hlakileng ea ...Bala haholoanyane -
Litlhokomelo bakeng sa RT-qPCR
Teko ea RT-qPCR e kenyelletsa tlhahiso ea RNA le tlhahlobo ea boleng, phetisetso ea morao-rao le mehato e meraro ea qPCR, mohato ka mong o na le litemoso tse ngata, re tla li hlahisa ka botlalo ka tlase.Ⅰ.Tlhahlobo ea boleng ba RNA Tekong ea RT-qPCR, kamora ho phethoa ha tlhahiso ea RNA, ...Bala haholoanyane -
Kenyelletso e phethahetseng ea nako ea nnete ea PCR
1. Kutloisiso ea pele Mothating ona, re hloka ho utloisisa likhopolo le mantsoe a mang, e le ho qoba ho etsa liphoso ka pel'a batho ba baholo, tse kang: P: Phapano ke efe pakeng tsa RT-PCR, qPCR, Real-time PCR, le RT-PCR ea nako ea sebele?Karabo: RT-PCR ke PCR e fetoletsoeng ka morao.Bala haholoanyane -
PCR, multiple PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR (1) - PCR
PCR, multiple PCR, In situ PCR, Reverse PCR, RT-PCR, qPCR (1)– PCR Re tla hlophisa likhopolo, mehato le lintlha tse fapaneng tsa PCR Ⅰ.PCR Polymerase Chain Reaction, e bitsoang PCR, ke theknoloji ea baeloji ea limolek'hule e sebelisetsoang ho holisa likhechana tse itseng tsa DNA.E ka nkoa e le ...Bala haholoanyane -
qRT-PCR molao-motheo oa liteko
RT-qPCR e entsoe ka theknoloji e tloaelehileng ea PCR.E eketsa lik'hemik'hale tsa fluorescent (lidae tsa fluorescent kapa li-fluorescent probes) ho sistimi ea karabelo ea PCR ea setso, mme e bona ts'ebetso ea PCR annealing le katoloso ka nako ea nnete ho latela mekhoa ea bona e fapaneng ea luminescent.Letšoao la fluorescent ...Bala haholoanyane -
Kakaretso e qaqileng ea mokhoa oa RT-PCR karabelo ea sistimi ea liteko
Ⅰ.Eketsa kutloisiso ea sistimi ea karabelo: 1. Arola RNA ea boleng bo holimo: Ts'ebetso ea cDNA e atlehileng e tsoa ho RNA ea boleng bo holimo.RNA ea boleng bo holimo e lokela ho netefatsa bonyane kakaretso e telele 'me ha e na li-inhibitors tse se nang li-enzyme tsa ho rekota, tse kang EDTA kapa SDS.Litšoaneleho...Bala haholoanyane
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