E hatisitsoe ke: CAS Key Laboratory ea Pathogen Microbiology le Immunology, Institute of Microbiology, Chinese Academy of Sciences
HAEBA: 69.504
Sehlahisoa se Thusang:Cell Total RNA Isolation Kit
Sehokelo sa mantlha:https://www.nature.com/articles/s41586-022-05271-2
Tšoaetso e nang le likokoana-hloko tse kang Ebola virus (EBOV) le kokoana-hloko ea Marburg e ka baka matšoao a matla a bophelo bo botle, ho akarelletsa le feberu ea haemorrhagic le ho hlōleha ha litho tse ngata.4.Mona re ile ra etsa qeto ea sebopeho sa Ebola virus L protheine e rarahaneng le tetrameric VP35 sebelisa cryo-electron microscopy (mmuso 1).Tlhahlobo ea sebopeho e senotse hore kokoana-hloko ea Ebola L e na le karolo e kentsoeng ea filovirus-specific e leng bohlokoa bakeng sa motsoako oa RNA, le hore VP35 e sebelisana haholo le sebaka sa N-terminal ea L ka li-protomer tse tharo tsa VP35 tetramer.
Ka pampiri ena, RNA eohle e ntšitsoe ho sebelisoa ForegeneCell Total RNA Isolation Kit'me mahloriso a RNA a lekantsoe ke Nanodrop ho 260 nm, ebe o etsa RT / qPCR ho lemoha.
■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.
Nako ea poso: Nov-04-2022
