Litaba tsa indasteri
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Ela hloko lintlha tse latelang ho habeli sekhahla sa katleho ea liteko tsa hau tsa RT-qPCR!
RT-qPCR ke teko ea mantlha ea baeloji ea limolek'hule, 'me motho e mong le e mong o tlameha ho e tseba.Haholo-holo e kenyelletsa mehato e meraro: ho ntšoa ha RNA, ho ngoloa ka morao ho cDNA, le PCR ea nako ea sebele ea fluorescent quantitative.Ha e thuse, ho etsahalang?Ho ka etsahala hore ebe ho na le bothata ba ...Bala haholoanyane -
Tlhahiso ea RNA, 260/230 e tlase haholo, 'me boleng ba ct ba pcr run bo phahame haholo.Joang ho e rarolla?
Karolelano e tlase ea A260/A230 hangata e bakoa ke litšila tse nang le maqhubu a phahameng a ho monya ho 230nm.Ha re boneng hore na litšila tsena li kenyeletsa eng: Litšila tse tloaelehileng Absorption wavelength Ratio effect Protein ~230nm le 280nm Phokotso ea nako e le 'ngoe ea A 260 /A 280 le A 260 /A ...Bala haholoanyane -
Fluorescent quantitative PCR (qPCR) - moralo oa pele
Litekong tsa qPCR, moralo oa primer le ona ke sehokelo sa bohlokoa haholo.Hore na li-primers li nepahetse kapa che ho amana haufi-ufi le hore na matla a ho holisa a fihla maemong, hore na lihlahisoa tse holisitsoeng li nepahetse, le hore na liphetho tsa liteko li teng.Kahoo mokhoa oa ho etsa ...Bala haholoanyane -
Li-additives tse tloaelehileng liketsong tsa PCR le mesebetsi ea tsona
Ke lumela hore motho e mong le e mong o tla lula a kopana le mathata a joalo kapa a joalo ha a etsa liketso tsa PCR, empa boholo ba bona bo ka aroloa ka mathata a mabeli a ka sehloohong: Ho holisa hanyenyane haholo ha template ea liphatsa tsa lefutso (amplification);Keketseho e ngata haholo ea mofuta oo e seng oa sepheo.Ho sebelisa li-additives ke e 'ngoe ea mefuta e tloaelehileng ...Bala haholoanyane -
Kakaretso ea litlhare tse 45 tsa kalafo ea lefutso tse rekisitsoeng lefatšeng ka bophara
Liphatsa tsa lefutso ke likarolo tsa motheo tsa lefutso tse laolang mekhoa.Ntle le liphatsa tsa lefutso tsa likokoana-hloko tse ling, tse entsoeng ka RNA, liphatsa tsa lefutso tsa lintho tse ngata li entsoe ka DNA .Bongata ba mafu a lintho tse phelang a bakoa ke tšebelisano pakeng tsa liphatsa tsa lefutso le tikoloho.Kalafo ea liphatsa tsa lefutso ha e le hantle e ka phekola ...Bala haholoanyane -
Nakong ea phekolo ea RNA, ke mang ea ka fetohang "moratuoa e mocha" oa indasteri |Thepa ea selemo
Mohloli: WuXi AppTec Lilemong tsa morao tjena, lefapha la kalafo ea RNA le bonts'itse mokhoa o phatlohileng- lilemong tse 5 tse fetileng feela, liphekolo tse 11 tsa RNA li amohetsoe ke FDA, mme palo ena e bile e feta kakaretso ea liphekolo tsa RNA tse amohetsoeng pele!Ha ho bapisoa le liphekolo tsa setso, phekolo ea RNA e ka...Bala haholoanyane -
PCR e tloaelehileng, PCR ea fluorescent, PCR ea digital;tlhahlobo ea melemo le mathata!
Litlhahiso tse 'maloa tsa phetoho historing ea theknoloji ea ho lemoha kelellong ea ka ke theknoloji ea immunolabeling e thehiloeng ho molao-motheo oa ho tlama ha antigen-antibody, theknoloji ea PCR le theknoloji ea tatellano.Kajeno re tla bua ka theknoloji ea PCR.Ho latela t...Bala haholoanyane -
Kakaretso| Ho tloha ka Phato 2022, litlhare tse 41 tsa kalafo ea lefutso li se li hlahisitsoe lefatšeng ka bophara.
Haufinyane tjena, litlhare tse tharo tsa kalafo ea liphatsa tsa lefutso li amohetsoe ho bapatsa, e leng: (1) Ka Phupu 21, 2022, PTC Therapeutics, Inc. (NASDAQ: PTCT) e phatlalalitse hore mofuta oa eona oa AAV gene therapy Upstaza™ o amohetsoe ke Khomishene ea Europe Ke pheko ea pele ea lefutso e rekisoang ka kotloloho ho ...Bala haholoanyane -
Nakong e Fetileng le Hona Joale ea CACLP
Ho tloha ka la 26 Mphalane ho isa la 28th, 2022, mokete o mocha oa meriana ea liteko le tlhahlobo ea in vitro - The 19th China China Association of Clinical Laboratory Practice Expo The 2nd China IVD Supply Chain Expo (CISCE) e ile ea tšoaroa ka katleho Nanchang Greenland International Expo Centre.FOREGENE Co...Bala haholoanyane -
Mochini oa PCR| Na u hlile ua utloisisa?
Mochini oa PCR| Na u hlile ua utloisisa?Thekenoloji ea PCR e hapileng Moputso oa Nobel Ka 1993, rasaense oa Leamerika Mulis o ile a fumana Khau ea Nobel ea Chemistry, 'me katleho ea hae e bile ho qaptjoa ha theknoloji ea PCR.Boselamose ba theknoloji ea PCR bo maemong a latelang: Taba ea pele, palo ea DNA ho ...Bala haholoanyane -
Thesis ea morao-rao ea Tlhaho: Sekaseka sebopeho le mochini oa DNA oa sesebelisoa se secha sa ho hlophisa liphatsa tsa lefutso ISRB
Lilemong tse leshome tse fetileng, theknoloji ea ho fetola liphatsa tsa lefutso e thehiloeng ho CRISPR e tsoetse pele ka potlako, 'me e sebelisitsoe ka katleho ho phekola mafu a lefutso le kankere litekong tsa batho.Ka nako e ts'oanang, bo-ramahlale lefats'eng ka bophara ba lula ba tlanya lisebelisoa tse ncha ka ho fetola liphatsa tsa lefutso ...Bala haholoanyane -
Phekolo ea liphatsa tsa lefutso, "tsoha" tsebe ka ho feletseng
Ho lahleheloa ke kutlo (HL) ke lefu le atileng haholo la bokooa ba kutlo ho batho.Linaheng tse tsoetseng pele, hoo e ka bang 80% ea batho ba sa utloeng litsebeng ka puo ea pele baneng ba bakoa ke liphatsa tsa lefutso.E tloaelehileng haholo ke bokooa ba mofuta o le mong (joalokaha ho bontšitsoe setšoantšong sa 1), liphetoho tsa liphatsa tsa lefutso tse 124 li fumanoe li amahanngoa le ...Bala haholoanyane
