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Mohale oa PCR
◮Botšepehi bo phahameng: 6linako tsa enzyme e tloaelehileng ea Taq;
◮Ka potlako alebelo la ho ntlafatsa:lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;
◮Ho ikamahanya le maemo ho feta template: e ka holisa ka nepo litempele tse fapaneng tse rarahaneng tsa DNA tse nang le litaba tse phahameng tsa GC mme ho thata ho li holisa;
◮E phahamengMatla a ho holisa: palo ea lipotoloho tsa amplification e tlase ho feta ea enzyme e tloaelehileng ea Taq;
◮Smatlafala Mamello ya tikoloho: e behiloe ho 37°C ka beke, e boloka ho feta90%ts'ebetso;
◮E na le ts'ebetso ea 5'→ 3' DNA polymerase le 5'→ 3' exonuclease, ntle le ts'ebetso ea 3'→5' ea exonuclease.
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Mohala
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Holimo