■Botšepehi bo phahameng: makhetlo a 6 ho feta a enzyme e tloaelehileng ea Taq;

■ Lebelo la ho hōlisa ka potlako : lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;

■ Ho ikamahanya le maemo ho hoholo ha template: E khona ho holisa li-template tse fapaneng tse rarahaneng tsa DNA ka holimoLitaba tsa GC;

■ Bokhoni bo phahameng ba ho holisa: lipotoloho tse tlase tsa matlafatso ho feta enzyme e tloaelehileng ea Taq;

■ Mamello ea tikoloho e matla haholoanyane: e behiloe ho 37°C bakeng sa beke, ho boloka ts'ebetso e fetang 90%;

■ E na le 5'→3'Mosebetsi oa DNA polymerase le 5'→3'ts'ebetso ea exonuclease, ntle le 3'→5'ts'ebetso ea exonuclease.

■ Sekoti se kopantsoe pele le Electrophoresis Loading Buffer, ka mor'a hore PCR e phethoe, sampuli e ka kenngoa ka ho toba bakeng sa electrophoresis;ts'ebetso e bonolo ebile e bonolo.