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Nako ea 'Nete PCR Easyᵀᴹ-SYBR Green I kit
E bonolo—2X PCR Mix ho fokotsa phoso ea liteko le nako ea ts'ebetso
Specific-optimized buffer le hot-start Taq enzyme e ka thibela ho holisoa ho sa hlakang le ho thehoa ha primer dimer.
Kutloelo-bohloko e phahameng - e khona ho bona likopi tse tlase tsa template
E sebetsa ka mokhoa o fapaneng - e tsamaellana le lisebelisoa tse ngata tsa nako ea nnete tsa PCR
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RT-PCR Easyᵀᴹ I(Mohato o le Mong)
◮Sesebelisoa sa mohato o le mong se etsa hore ho fetoleloa ka morao le PCR ho etsoe ka tube e le 'ngoe.E hloka feela ho eketsa template ea RNA, li-primer tsa PCR le RNase-Free ddH2O.
◮Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
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RT-PCR Easyᵀᴹ II(Mehato e Meli)
◮Tlhahlobo ea nako ea 'nete ea RNA e ka etsoa kapele le ka nepo.
◮Setsi sena se sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
◮ Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
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Nako ea 'Nete PCR Easyᵀᴹ-Taqman
◮E bonolo-2 × PCR Mix ho fokotsa phoso ea liteko le nako ea ts'ebetso
◮Specific-optimized buffer le hot-start Taq enzyme e ka thibela ho holisoa ho sa hlakang le ho thehoa ha primer dimer.
◮Kutloelo-bohloko e phahameng - e khona ho bona likopi tse tlase tsa template
◮E sebetsa ka mokhoa o fapaneng - e tsamaellana le lisebelisoa tse ngata tsa nako ea nnete tsa PCR
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PCR Heroᵀᴹ (Ka dae)
◮Botšepehi bo phahameng: makhetlo a 6 ho feta a enzyme e tloaelehileng ea Taq;
◮Lebelo la amplification le potlakileng
◮Ho ikamahanya le maemo ho feta template
◮Bokhoni bo phahameng ba amplification
◮Ho mamella tikoloho ho matla: ho behoa ho 37 ° C ka beke, ho boloka mosebetsi o fetang 90%;
◮E na le ts'ebetso ea 5'→ 3' DNA polymerase le 5'→ 3' exonuclease, ntle le ts'ebetso ea 3'→5' ea exonuclease.
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700bp DNA Lere
◮700bp DNA Ladder ke sehlahisoa se seng se loketse ho sebelisoa 'me se se se na le 1x Loading Buffer.U ka nka ka ho toba palo e nepahetseng ea Marker bakeng sa electrophoresis ho latela litlhoko tsa liteko.
700bpDNA Lesupa Ⅰ e na le lihlopha tse 7 tsa DNA, lihlopha tsa DNA ke: 100bp (50ng/5μl), 200bp (30ng/5μl), 300bp (30ng/5μl), 400bp (40ng/5μl), 500bp (50ng/50) , 500bp (250/5) bp(35ng/5μl).
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2000bp DNA Lere
◮ 2000bp DNA Ladder ke sehlahisoa se seng se loketse ho sebelisoa 'me se se se na le 1x Loading Buffer.U ka nka ka ho toba palo e nepahetseng ea Marker bakeng sa electrophoresis ho latela litlhoko tsa liteko.BM2000 MARSERS
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5000bp DNA Lere
◮ 2000bp DNA Lereke sehlahisoa se seng se loketse ho sebelisoa 'me se se se na le 1x Loading Buffer.U ka nka ka ho toba palo e nepahetseng ea Marker bakeng sa electrophoresis ho latela litlhoko tsa liteko.BM5000 DNA Marker e na le lihlopha tse 8 tsa DNA, lihlopha tsa DNA ke: 100bp (50ng/5μl), 250bp(50ng/5μl), 500bp(50ng/5μl), 750bp(75ng/5μl), 1000 )ng0/5μl), 1000 )ng0/5μl, 1000)ng/5μl ), 3000bp(30ng/5μl), 5000bp(50ng/5μl).
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PCR Easyᵀᴹ (e nang le dae)
Mohale oa 2 × PCRTM Sistimi ea Mix e na le mamello e phahameng ho li-inhibitors tsa PCR ho feta sistimi e tloaelehileng ea PCR Mix, 'me e khona ho sebetsana habonolo le ntlafatso ea PCR ea litempele tse fapaneng tse rarahaneng.Sistimi e ikhethang ea karabelo le ts'ebetso e phahameng ea Taq Hero li etsa hore karabelo ea PCR e be le bokhoni bo phahameng ba ho holisa, boits'oaro le kutlo.
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PCR Easy
Sistimi e ikhethang ea karabelo le ts'ebetso e phahameng ea Taq DNA Polymerase li etsa hore karabelo ea PCR e be le katleho e phahameng ea ho holisa, boits'oaro le kutlo.
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Mohale oa PCR
◮Botšepehi bo phahameng: 6linako tsa enzyme e tloaelehileng ea Taq;
◮Ka potlako alebelo la ho ntlafatsa:lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;
◮Ho ikamahanya le maemo ho feta template: e ka holisa ka nepo litempele tse fapaneng tse rarahaneng tsa DNA tse nang le litaba tse phahameng tsa GC mme ho thata ho li holisa;
◮E phahamengMatla a ho holisa: palo ea lipotoloho tsa amplification e tlase ho feta ea enzyme e tloaelehileng ea Taq;
◮Smatlafala Mamello ya tikoloho: e behiloe ho 37°C ka beke, e boloka ho feta90%ts'ebetso;
◮E na le ts'ebetso ea 5'→ 3' DNA polymerase le 5'→ 3' exonuclease, ntle le ts'ebetso ea 3'→5' ea exonuclease.
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QuickEasyᵀᴹ Nako ea 'Nete PCR Kit-Taqman
◮Sistimi e ikhethileng ea PCR e etsa 2×QuickEasyTMReal PCR Mix-Taqman e tsamaellana haholoanyane.
◮Hot-start Foregene HS Taq Polymerase e na le bokhoni bo phahameng ba ho holisa, kutlo e phahameng ea amplification, le ho hlaka ho hoholo ho holimo.
◮2 × QuickEasyTMReal PCR Mix -Taqman e sebelisa sistimi e ikhethileng e ntlafalitsoeng ke Foregene ho ntlafatsa kutlo le ho hlaka ha tlhahlobo e ikhethileng ea tatellano, e ka sebelisoang ho etsa qeto ea ho fapana ha linomoro tsa genotyping le ho kopitsa le ho fumana liphetho tse nepahetseng.
◮Sehlahisoa sena se tla le dae ea ka hare ea ROX, e ka sebelisoang ho tlosa semelo sa matšoao le phoso ea matšoao lipakeng tsa liliba, tse loketseng bareki ho li sebelisa ka mefuta e fapaneng ea lisebelisoa tsa PCR.
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