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Plant leaf Direct PCR kit (ntle le Sampling Tools) Protocol Direct PCR from Plant Material

Tlhaloso ea Kit:

Sebelisa ka ho toba lekhasi kapa lysate ea lekhasi e le template ea ho hōlisa PCR, ntle le ho ntša DNA e arohaneng, ka potlako le ka kutloisiso e phahameng.

Ha ho tlhoekiso ea DNA e jang nako le e turang (Nako, mosebetsi le litšenyehelo li bolokiloe!)

Boitsebiso bo fokolang

E bonolo - sampole e ka ba tlas'a karabelo ea PCR kapa karabelo ea lysis ntle le ho seha kapa ho sila

2 X Mix-ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi

Tokiso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa ka metsotso e 50

High throughput-lysis reaction e ka phethoa ka poleiti ea PCR ea seliba tse 96

matla a foregene


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso

Sehlahisoa sena se sebelisa mokhoa o ikhethileng oa lysis buffer ho lisa makhasi a semela.Lysate e ka sebelisoa e le template ntle le tlhoekiso.Kamora ho kenyelletsa li-primers, PCR Mix ea kit ena e ka sebelisoa bakeng sa ho holisa.

Litlhaloso

0×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Likarolo tsa Kit

Karolo ea I

Buffer P1

Buffer P2

6×DNA Loading Buffer

Karolo ea II

2× Lekhasi la PCR le bonoloTMKopanya

Likarolo le melemo

■ Ha ho tlhoekiso ea DNA e jang nako le e theko e boima
■ Boitsebiso bo fokolang
■ E bonolo-sampole e ka ba tlas'a PCR reaction kapa lysis reaction ntle le ho seha kapa ho sila
■ 2 × Mix, ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
■ Tokisetso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa bonyane ka metsotso e 50.
■ Boitšoaro bo phahameng ba throughput-lysis bo ka phethoa ka poleiti ea PCR ea seliba sa 96

Litekanyetso tsa kit

Kopo: boitsebiso bo fetotsoeng ka liphatsa tsa lefutso, genotyping, joalo-joalo.
Mohlala: lekhasi la semela
Tekanyo: Diameter 2-3mm (mokhoa o tobileng), bophara ba 5-7mm (mokhoa oa lysis)
Sebaka sa ho lemoha: sekhechana sa sepheo ≤1kb

Phallo ea mosebetsi

Semela Leaf Direct PCR Kit04

Electropherogram

Semela Lekhasi Direct PCR Kit05

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