Lekhasi la semela Direct PCR kit-UNG (ntle le Lisebelisoa tsa Sampling) Protocol Direct PCR ho tsoa ho Plant Material.
Litlhaloso
Ho ipapisitsoe le Plant Leaf Direct PCR kit, dUTP e sebelisoa sebakeng sa dTTP, 'me enzyme ea UNG e ka theolang thempleite e nang le dUTP ea eketsoa.Ka tsela ena, ho netefatsoa le ho nepahala ha ho holisa, 'me bothata ba tšilafalo ea sehlahisoa sa PCR bo ka hlahang nakong ea tlhahlobo e kholo ea liphatsa tsa lefutso bo thibeloa.
Litlhaloso
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Likarolo tsa Kit
Karolo ea I | Buffer P1 |
Buffer P2 | |
6×DNA Loading Buffer | |
Karolo ea II | 2× Lekhasi la PCR le bonoloTMMotsoako(UNG) |
Likarolo le melemo
■ Ha ho tlhoekiso ea DNA e jang nako le e theko e boima
■ Boitsebiso bo fokolang
■ E bonolo-sampole e ka ba tlas'a PCR reaction kapa lysis reaction ntle le ho seha kapa ho sila
■ 2 × Mix, ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
■ Tokisetso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa bonyane ka metsotso e 50.
■ Boitšoaro bo phahameng ba throughput-lysis bo ka phethoa ka poleiti ea PCR ea seliba sa 96
Litekanyetso tsa kit
Kopo: boitsebiso bo fetotsoeng ka liphatsa tsa lefutso, genotyping, joalo-joalo.
Mohlala: lekhasi la semela
Tekanyo: Diameter 2-3mm (mokhoa o tobileng), bophara ba 5-7mm (mokhoa oa lysis)
Sebaka sa ho lemoha: sekhechana sa sepheo ≤1kb
Phallo ea mosebetsi
Electropherogram
Libeke tse peli tsa ho fumana liphetho tsa PCR Mix tlas'a boemo ba tšilafalo ea sehlahisoa sa PCR.