-
Semela Kakaretso ea RNA Isolate Kit
Thepa e sebelisa spin ea kholomo le foromo e ntlafalitsoeng ke Foregene, e ka ntšang bohloeki bo phahameng le boleng bo phahameng ba RNA ho tsoa liseleng tse fapaneng tsa semela tse nang le polysaccharides e tlase le polyphenols. Bakeng sa mehlala ea semela e nang le polysaccharides e phahameng kapa polyphenols, ho kgothaletswa hore o sebelise Plant Total RNA Isolate Plus Kit ho fumana liphetho tse ntle tsa RNA. Thepa e fana ka DNA-Cleaning kholomo e ka tlosang genomic DNA habonolo ho tsoa ho supernatant le lysate ea lisele. Lenaneo la RNA feela le ka tlama RNA hantle. Thepa e ka sebetsana le palo e kholo ea mehlala ka nako e le 'ngoe.
Sisteme eohle ha e na RNase, ka hona RNA e hloekisitsoeng e ke ke ea senyeha. Buffer PRW1 le Buffer PRW2 li ka netefatsa hore RNA e fumanoeng ha e silafatsoe ke protheine, DNA, li-ion le metsoako ea manyolo.
