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RT Easyᵀᴹ II Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa nako ea 'nete ea PCR
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 20 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃, 'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 60%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
- 2× RT KAPA-E bonoloTM Mix e kentse li-primers tse ntlafalitsoeng tsa khatiso (Random Primer, Oligo(dT)18 Sethome).
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Holimo