Nako ea 'Nete PCR Easyᵀᴹ-SYBR Green I kit
Tlhaloso ea Kit:
E bonolo—2X PCR Mix ho fokotsa phoso ea liteko le nako ea ts'ebetso
Specific-optimized buffer le hot-start Taq enzyme e ka thibela ho holisoa ho sa hlakang le ho thehoa ha primer dimer.
Kutloelo-bohloko e phahameng - e khona ho bona likopi tse tlase tsa template
E sebetsa ka mokhoa o fapaneng - e tsamaellana le lisebelisoa tse ngata tsa nako ea nnete tsa PCR
Litlhaloso tsa Kit
2 × PCR ea 'Nete e BonoloTMMix-SYBR e fanoeng ke Real Time PCR EasyTM-SYBR Green I Kit ke sistimi e ncha ea premix e sebelisang SYBR Green I bakeng sa karabelo ea amplification ea Nako ea 'Nete ea PCR, e ka ntlafatsang haholo boleng ba sehlahisoa le kutloelo-bohloko.ROX e fanoa e le dae ea taolo ea kahare.Matla a fluorescence a kit ena ke makhetlo a 3-5 ho feta a lihlahisoa tse tšoanang, tse ka bang bonolo haholoanyane 'me ka mokhoa o hlakileng li bonahatsa mahloriso a DNA ea template.
2 × PCR ea 'Nete e BonoloTMMix-SYBR e na le Taq DNA Polymerase e ikhethang ea Foregene.Ha e bapisoa le li-enzyme tse tloaelehileng tsa Taq, e na le melemo ea matla a ho holisa haholo, bokhoni bo khethehileng ba ho holisa le sekhahla se tlase sa ho se lumellane.E ka fokotsa matlafatso e sa tobang le ho ntlafatsa ho nepahala ha PCR.
Litlhaloso
| Nako ea 'Nete PCR Easyᵀᴹ-SYBR Green I Kit | ||||
| Sebopeho sa Kit(20μl system) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
| 200T | 500T | 1000T | 2000T | |
| 2 × PCR ea 'Nete e BonoloTMKopanya-SYBR | 1 ml × 2 | 1.7 ml × 3 | 1.7 ml × 6 | 1.7 ml × 12 |
| 50 × ROX Reference Dye | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
| DNase-Free ddH2O | 1.7 ml | 1.7 ml × 2 | 10 ml | 20 ml |
| Taelo | 1 | 1 | 1 | 1 |
Likarolo le melemo
■ E bonolo—2X PCR Mix ho fokotsa phoso ea liteko le nako ea ts'ebetso
■ Specific-optimized buffer le hot-start Taq enzyme e ka thibela amplification e sa khetheheng le sebopeho sa primer dimer.
■ Kutlo e phahameng-e ka lemoha likopi tse tlase tsa template
■ Tšebeliso e ntle ea lintho tse ngata—e lumellana le lisebelisoa tse ngata tsa nako ea sebele tsa PCR
Sesebelisoa sa Kit
qPCR tlhahlobo
Phallo ea mosebetsi
Litšoantšo
Polokelo le shelf bophelo
Sekhahla sena se lokela ho bolokoa hole le khanya 'me se lokela ho bolokoa ho -20 ℃.Haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ ka nako e khutšoanyane (matsatsi a 10).
