- E 'ngoe-mohato oa mohato o nolofalletsa ho ngoloa ka morao le PCR ho etsoa ka tube e le 'ngoe, ho hlokahala feela ho eketsa template ea RNA, li-primers tse khethehileng tsa PCR le RNase-Free ddH.2O.
- Tlhahlobo ea nako ea 'nete ea palo ea RNA ea vaerase kapa trace RNA e ka etsoa kapele le ka nepo.
- Thepa e sebelisa mochini o ikhethileng oa Foregene reverse transcription reagent le Foregene HotStar Taq DNA Polymerase e kopantsoeng le sistimi e ikhethang ea karabelo ho ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.
- Sistimi e ntlafalitsoeng ea karabelo e etsa hore karabelo e be le kutlo e phahameng ea ho lemoha, botsitso bo matla ba mocheso, le mamello e betere.
- RT-qPCR e bonoloTM(Mohato o le mong) -Taqman kit e tla le dae ea ka hare ea ROX, e ka sebelisoang ho tlosa semelo sa matšoao le liphoso tsa matšoao lipakeng tsa liliba, tse loketseng basebelisi ba ho qetela ho li sebelisa mefuteng e fapaneng ea lisebelisoa tsa PCR tse ngata.