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Lnc-RT Heroᵀᴹ I(With gDNase)(Super Premix for first-strand cDNA synthesis from lncRNA)
Super Premix bakeng sa motsoako oa pele oa cDNA ho tloha lncRNA
Katse.No.RTL-09098/09099
Sistimi e potlakileng le e nang le kutloelo-bohloko ea morao-rao bakeng sa ho hlahisa cDNA ea mofuta oa pele ho tsoa ho lncRNA.
Tlosa gDNA ka metsotso e 2.
Metsotso e 15 ho phethela motsoako oa cDNA ea pele
Sistimi ea mongolo oa reverse reverse
Botsitso bo phahameng ba mocheso, mocheso o nepahetseng oa karabelo ke 42 ℃, 'me e ntse e na le ts'ebetso e ntle ea ho fetolela ho 50 ℃.
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RT Easyᵀᴹ I Master Premix bakeng sa motsoako oa pele oa cDNA
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 25 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃,'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 45%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
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RT Easy II(e nang le gDNase) Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa Real Time PCR e nang le gDNase
-Bokhoni bo botle ba ho tlosa gDNA, e ka tlosa gDNA ka template ka hare ho metsotso ea 2.
-Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 15 feela ho phethela motsoako oa cDNA ea pele.
Li-templates tse rarahaneng: litempele tse nang le litaba tse phahameng tsa GC le sebopeho sa bobeli se rarahaneng le tsona li ka khutlisoa ka katleho e phahameng.
-High-sensitivity reverse transcription system, litempele tsa pg-level le tsona li ka fumana cDNA ea boleng bo holimo.
-Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso o nepahetseng oa karabelo ke 42℃, 'me e ntse e na le ts'ebetso e ntle ea ho fetolela ho 50℃.
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RT Easyᵀᴹ II Master Premix bakeng sa motsoako oa pele oa cDNA bakeng sa nako ea 'nete ea PCR
- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 20 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃, 'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 60%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.
- 2× RT KAPA-E bonoloTM Mix e kentse li-primers tse ntlafalitsoeng tsa khatiso (Random Primer, Oligo(dT)18 Sethome).
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Mohala
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Holimo