Viral DNA&RNA Isolation Kit Viral DNA le RNA Extraction Preparation Kits
Litlhaloso
50 Preps, 200 Preps
Viral RNA Nucleic acid Purification Extraction Isolation Kit e sebelisa kholomo le foromo e entsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo ho tsoa lisampoleng tse kang plasma, serum, mokelikeli o se nang lisele tsa 'mele, le cell culture supernatant.Kit e eketsa ka ho khetheha Linear Acrylamide, e ka nkang RNA e nyane habonolo ho tsoa lisampoleng.RNA-Feela Column e ka tlama RNA hantle.Kit e ka sebetsana le palo e kholo ea lisampole ka nako e le 'ngoe.
Thepa eohle ha e na RNase, kahoo RNA e hloekisitsoeng e ke ke ea senyeha.Buffer viRW1 le Buffer viRW2 e ka etsa bonnete ba hore varal nucleic acid e fumanoeng ha e na protheine, nuclease kapa litšila tse ling, e ka sebelisoang ka kotloloho bakeng sa liteko tsa baeloji ea limolek'hule.
Likarolo tsa Kit
| Linear Acrylamide |
| Buffer DRL |
| Buffer RW1, Buffer RW2 |
| RNase-Free ddH2O |
| DNA/RNA Column |
| Litaelo |
Likarolo le melemo
■ Ts'ebetso ka mocheso oa kamore (15-25 ℃) ho pholletsa le ts'ebetso eohle, ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Complete Kit RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ Tlhahiso e phahameng ea nucleic acid: DNA / RNA-feela Column le foromo e ikhethang e ka hloekisa DNA le RNA ka katleho.
■ Sample e kholo ea matla a ho sebetsa: ho fihlela ho 200μl lisampole li ka sebetsoa nako le nako.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa nakong ea metsotso e 20.
■ Tšireletseho: ha ho na reagent ea tlhaho e hlokahalang.
■ Boleng bo phahameng: Likaroloana tsa RNA tse hloekisitsoeng li hloekile haholo, ha li na protheine le litšila tse ling, 'me li ka kopana le mekhoa e fapaneng ea liteko tse tlase.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha viral nucleic acid ka lisampole tse kang plasma, serum, mokelikeli oa 'mele o se nang lisele le setso sa sele.
Phallo ea mosebetsi
Setšoantšo
Storage le Shelf bophelo
■ Letlapa lena le ka bolokoa likhoeli tse 24 tlas'a maemo a omileng mocheso oa kamore (15-25℃);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ka 2–8℃.
■ Linear Acrylamide tharollo ka bolokoa mocheso kamoreng matsatsi a 7;ka mor'a ho amohela khiti, ka kopo, e ntše 'me u e boloke ho -20°C.
■ Ka mor'a ho eketsa Linear Acrylamide ho Buffer DRL, e ka bolokoa ho 2-8 ° C ho fihlela ho 48h.Ka kopo sebelisa tharollo e seng e entsoe.
Tataiso ea Tlhahlobo ea Mathata
Se latelang ke tlhahlobo ea mathata a ka 'nang a kopana le ho ntšoa ha kokoana-hloko ea DNA/RNA, ka tšepo ea ho thusa litekong tsa hau.Ho feta moo, bakeng sa mathata a mang a liteko kapa a tekheniki ntle le litaelo tsa ts'ebetso le tlhahlobo ea mathata, re fane ka tšehetso ea tekheniki ho u thusa.Haeba u na le litlhoko, ka kopo ikopanye le rona: 028-83360257 kapa E-mail:
Tech@foregene.com.
Ha ho tlhahiso ea nucleic acid kapa lihlahisoa tse tlase tsa nucleic acid
Hangata ho na le lintlha tse ngata tse amang ts'ebetso ea ho hlaphoheloa, joalo ka: sampole ea nucleic acid content, mokhoa oa ts'ebetso, molumo oa elution, joalo-joalo.
Tlhahlobo ea lisosa tse tloaelehileng:
1. Ho hlatsoa leqhoa kapa mocheso o tlaase (4 ° C) centrifugation e ne e etsoa nakong ea ts'ebetso.
Tlhahiso: Sebetsa ka mocheso oa kamore (15-25 ° C) ho pholletsa le ts'ebetso eohle, u se ke ua hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
2. Mohlala o ne o bolokiloe ka mokhoa o sa lokelang kapa mohlala o bolokiloe nako e telele haholo.
Keletso: Boloka lisampole ho -80°C 'me u qobe serame khafetsa le ho qhibiliha;leka ho sebelisa lisampole tse sa tsoa bokelloa bakeng sa ho ntša nucleic acid.
3. Sample e sa lekaneng ea lysis.
Keletso: Ka kopo etsa bonnete ba hore sampole le tharollo ea ho sebetsa ea lysis li tsoakane ka botlalo 'me li kenngoa mocheso oa kamore (15-25 ° C) metsotso e 10.
4. Keketso e fosahetseng ea eluent.
Tlhahiso: Etsa bonnete ba hore RNase-Free ddH2O e kentsoe ka tsela e theohang bohareng ba lera la tlhoekiso, 'me u se ke ua e lahlela lesale la ho hloekisa.
5. Molumo o nepahetseng oa ethanol e felletseng ha oa eketsoa Buffer RW2.
Tlhahiso: Ka kopo latela litaelo, eketsa molumo o nepahetseng oa ethanol e feletseng ho Buffer RW2 'me u kopanye hantle pele u sebelisa khiti.
6. Molumo o sa lokelang oa sampole.
Tlhahiso: 200µl ea sampole e sebetsoa bakeng sa 500µl e 'ngoe le e 'ngoe ea Buffer DRL.Ts'ebetso e feteletseng ea sampole e tla fella ka chai e tlase ea ho ntšoa ha nucleic acid.
7. Molumo oa lentsoe o sa nepahalang kapa tlhahiso e sa fellang.
Keletso: Molumo o hlakileng oa kholomo ea tlhoekiso ke 30-50μl;haeba phello ea elution e sa khotsofatse, ho kgothaletswa ho eketsa nako ka mocheso oa kamore ka mor'a ho eketsa RNase-Free ddH2O ea preheated, joalo ka 5-10min.
8. Ethanol e sala kholomong ka mor'a ho hlatsoa ka Buffer RW2.
Tlhahiso: Haeba ethanol e sala ka mor'a centrifugation le Buffer RW2 bakeng sa metsotso e 2, kholomo e ka behoa mocheso oa kamore metsotso e 5 ka mor'a centrifugation ho tlosa ethanol e setseng ka botlalo.
Nucleic acid e hloekisitsoeng e fokotsehile
Boleng ba nucleic acid e hloekisitsoeng e amana le ho boloka sampole, tšilafalo ea RNase, ts'ebetso le lintlha tse ling.Tlhahlobo ea lisosa tse tloaelehileng:
1. Li-sampuli tse bokelitsoeng ha lia ka tsa bolokoa ka nako.
Tlhahiso: Haeba sampole e sa sebelisoe ka nako ka mor'a ho bokelloa, ka kopo e boloke ho -80°C mocheso o tlase hang-hang.Bakeng sa ho ntša RNA, leka ho sebelisa lisampole tse sa tsoa bokelloa.
2. Bokella lisampole 'me u hatsele le ho qhibiliha khafetsa.
Tlhahiso: Qoba ho hatsela le ho qhibiliha (e seng ho feta hanngoe) nakong ea ho bokella le ho boloka lisampole, ho seng joalo tlhahiso ea nucleic acid e tla fokotseha.
3. RNase e hlahisoa ka kamoreng ea ts'ebetso kapa li-gloves tse lahliloeng, limaske, joalo-joalo ha li apesoa.
Keletso: Liteko tsa ho ntša RNA li etsoa hantle ka phaposing e arohaneng ea tšebetso ea RNA, 'me tafole ea laboratori e lokela ho hloekisoa pele ho teko.
Apara liatlana le limaske tse lahliloeng nakong ea liteko ho qoba ho senyeha ha RNA ho bakoang ke ho kenngoa ha RNase ho isa tekanyong e kholo.
4. Reagent e ne e silafalitsoe ke RNase nakong ea tšebeliso.
Keletso: Kenya sebaka ka Viral DNA/RNA Isolation Kit e ncha bakeng sa liteko tse amanang.
5. Li-tubes tsa centrifuge le malebela a pipette a sebelisoang bakeng sa ho fetola RNA li silafalitsoe ke RNase.
Tlhahiso: Etsa bonnete ba hore li-tubes tsa centrifuge, malebela a pipette, pipette, joalo-joalo tse sebelisetsoang ho ntša RNA kaofela ha li na RNase.
Asiti e hloekisitsoeng ea nucleic e ama liteko tse tlase
DNA le RNA e hloekisitsoeng ke kholomo ea tlhoekiso, haeba ion ea letsoai le protheine e phahame haholo, e tla ama liteko tse tlase, tse kang: PCR amplification, reverse transcription, joalo-joalo.
1. DNA e hlakisitsoeng le RNA li na le li-ion tse setseng tsa letsoai.
Tlhahiso: Etsa bonnete ba hore molumo o nepahetseng oa ethanol e feletseng e kenngoa ho Buffer RW2, 'me u hlatsoe kholomo ea tlhoekiso habeli ka lebelo la centrifugation le boletsoeng litaelong tsa ts'ebetso;Etsa centrifugation ho fokotsa tšilafalo ea ion ea letsoai.
2. DNA e hlakisitsoeng le RNA li na le masalla a ethanol.
Tlhahiso: Ka mor'a ho netefatsa ho hlatsoa ka Buffer RW2, etsa centrifugation e se nang letho ka lebelo la centrifugation ka litaelo tsa ts'ebetso;haeba ho ntse ho e-na le masala a ethanol, u ka centrifuge tube e se nang letho ebe u e beha mocheso oa kamore ka metsotso e 5 ho tlosa masala a ethanol ka tekanyo e kholo ka ho fetisisa.
Litaelo:
Viral DNA&RNA Isolation Kit Instruction Manual
