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Zebra Fish Direct PCR Kit
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho tsoa kapele DNA ea genomic ho tsoa ho li-zebrafish le lisele tse ling tsa tlhapi ea metsi a hloekileng, mapheoana a mohatla kapa mehlala ea mahe a tlhapi bakeng sa liphetoho tsa PCR, ka hona e loketse liteko tsa lefutso tse kholo. Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe nakong ea metsotso e 10-30 ho 65 ° C. Ha ho lits'ebetso tse ling tse kang ho tlosoa ha protheine le RNA tse hlokahalang, 'me DNA e lokollotsoeng e ka sebelisoa e le template ea karabelo ea PCR.
2 × PCR Easy TM Mix e na le mamello e matla ho li-inhibitor tsa PCR, 'me e ka sebelisa lysate ea sampole e tla hlahlojoa e le template bakeng sa kholiso e sebetsang hantle le e ikhethang. Reagent ena e na le ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer le stabilizer. E sebelisoa hammoho le buffer ea lysis e ka lemoha mehlala ka potlako le ka mokhoa o bonolo, 'me e na le litšobotsi tsa kutloisiso e phahameng, ho khetheha ho matla le botsitso bo botle.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
