Direct PCR ke eng

Direct PCR ke mokhoa oa DNA amplification ka kotloloho ho tlohamadi, lesela la phoofolo,rat mohatla,sele, tsebe ea likhoto,qoaha tlhapi, mahe a tlhapi,makhasi a semela,dipeokapa sampole e 'ngoe ea mantlha ea lisele tsa tlhaho ntle le ho etsa mehato ea ho itšehla thajana ea DNA le ho itlhoekisa.Mokhoa oa Direct PCR o ka fokotsa nako ea liteko haholo, le litšenyehelo tsa merero ea genotyping le ea bongata bo bongata.E boetse e fana ka khetho e betere ha u tobane le mathata a ho holisa sampole e nyane haholo moo mohato oa tlhoekiso o ka lebisang ho tahlehelo ea sampole.

FOREGENE's PCR Mix e na le khanyetso e matla ea khatello ea maikutlo, ka lebaka la FOREGENE matla a patented Taq enzyme( tumello ea molao: ZL20161034512.0 (Chaena), EP3450560 (Europe), PCT/CN2017/082154 (US), patent 6894926 (Japan)Enzyme ea eona ea Taq e sebelisa thekenoloji ea DNA recombination ho kopanya hape sebaka sa sebopeho se tlamang sa DNA sa archaeal nucleic acid e tlamang protheine e nang le Taq DNA polymerase e thibelang mocheso ho etsa template ea DNA e nang le kamano e eketsehileng.Taq e ntlafetseng e chesang e bolokang mesebetsi e sa tšoaneng ea DNA polymerase ea pele. Enzyme ena ea Taq e ka tlama ho template ea DNA le ho qala motsoako oa DNA ka li-template tse tlaase tsa mahloriso le libaka tse rarahaneng.

For mohlala,itho bonolo joalo ka ho eketsa ea hausele,semela (lekhasi le lenyane, motso kapa peo) kapa sampole ea phoofolo hobuffer, le ho eketsa e ikhethangmonghali kopanya leitsengli-primer ka har'a li-tubes tsa PCR le ho li tsamaisa ka sebaesekele se futhumatsang.Ho sebetsa hantle ha lisebelisoa ho loketse tlhahlobo e kholo ea sampole moo ho boloka nako ho leng bohlokoa haholo.

Sistimi ea karabelo ea PCR e nang le enzyme ea UNG le dUTP e khethoa ho latela litlhoko tsa liteko, tse ka qobang tšilafalo e bakiloeng ke lihlahisoa tsa amplification tsa PCR.