- Sistimi e sebetsang hantle ea ho fetolela, ho nka metsotso e 25 feela ho phethela motsoako oa cDNA ea pele.
- Sistimi e phahameng ea sensitivity reverse transcript, litempele tsa boemo ba pg le tsona li ka fumana cDNA ea boleng bo holimo.
- Sistimi ea reverse transcript e na le botsitso bo phahameng ba mocheso, mocheso oa karabelo ea sistimi e ka ba holimo ho 50 ℃,'me e na le ts'ebetso e ntle ea ho fetolela ka morao.
- Esita le disampole tse sa hloekang tsa RNA (joala bo fihlang ho 45%, letsoai la guanidine ho fihla ho 750mM) li ka ba tlas'a karabelo e fapaneng ea transcript.