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Mouse Mohatla SuperDirect PCR Kit
Setsi sena se sebelisa sistimi e ikhethileng ea lysis ho lokolla DNA ea genomic kapele ho tsoa mohatleng oa toeba le litsebeng tsa litsebe bakeng sa karabelo ea PCR, ka hona e loketse liteko tsa lefutso tse kholo.
Ts'ebetso ea ho lokolla genomic DNA ho buffer ea lysis e phethetsoe pele ho metsotso e 10-30 mochesong oa kamore (20-25 ° C). Ha ho hlokahale ho futhumatsa, mme lits'ebetso tse ling tse kang ho tlosa protheine le RNA ha li hlokehe. Palo ea DNA e lokollotsoeng e ka sebelisoa e le Setšoantšo sa karabelo ea PCR.
2 × M-PCR Easy TM Mix e na le mamello e matla ho li-inhibitor tsa PCR, 'me e ka sebelisa lysate ea sampole e tla hlahlojoa e le template bakeng sa kholiso e sebetsang hantle le e ikhethang. Reagent ena e na le Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer le stabilizer.
D-Taq DNA polymerase ke DNA polymerase e ntlafalitsoeng ka ho khetheha ke Foregene bakeng sa liphetoho tse tobileng tsa PCR. D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho matlafatsa bongata ba DNA ka mekhoa e fapaneng e fapaneng ea tšebetso,' me lebelo la ho matlafatsa le ka fihla ho 2Kb / min, e loketseng haholo karabelo ea Direct PCR.
