Tlhahlobo e latelang ea mathata a ka bang teng kaBuccalswab/FTA card DNA extraction is helpful to your experiment. In addition, for other experimental or technical problems in addition to operating instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us at: 028-83360257 or E-mali:Tech@foregene.com.

Kholomo ea tlhoekiso e koalehile

Ka kit ena, ts'ebetsong ea ts'ebetso ea DNA ea genomic, kholomo ea tlhoekiso e adsorbed ka ho toba ka sampuli ea enzymatic lysis motsoako ntle le mohato oa centrifugation, 'me mohala oa tlhoekiso o ka thibeloa ka lebaka la enzymization e sa fellang le viscosity e phahameng ea sampuli.

Lisosa tse latelang tse ka hlahang ke tse latelang:

1. Tshilo e sa fellang ya enzymatic ya disampole tsa dithishu.

Keletso: Nako ea ho sebetsa ea mohlala ea Foregene Protease e ka atolosoa ka nepo kapa supernatant e ka nkuoa kamora centrifugation ka 12,000 rpm (~13,400× g) bakeng sa metsotso e 5.

2. Tšebeliso e feteletseng ea lisampole tsa linama kapa lisele tse kholo.

Keletso: Ho molemo ho se fete 1Buccal swab ka mohlala;haeba sampole e le kholo haholo, eketsa litekanyetso tsa Buffer ST1, Foregene Protease, buffer ST2 ka nepo.

3. Mohlala oa viscosity o phahame haholo.

Keletso: lisampole li ka hlapolloa ka mokhoa o nepahetseng ka 10 mM ea Tris-HCl pele ho ho ntšoa ha genomic DNA.

4. Likhechana tsa karete ea mali li anngoe.

Keletso: Nako ea nakoana ea centrifugation ea mohato oa 6 oa sebaka sa mali (FTA Card) genomic extraction e ka atolosoa ka nepo.

Kotulo e tlase kapa ha e na DNA

Hangata ho na le lintlha tse fapaneng tse amang tlhahiso ea DNA ea genomic, ho kenyelletsa le tšimoloho ea mohlala, maemo a polokelo ea sampole, ho lokisoa ha mohlala, ho qhekella, joalo-joalo.

Genomic DNA e ke ke ea fumanoa nakong ea ho ntšoa

Lisosa tse ka bang teng ke tse latelang:

1. Paballo e fosahetseng ea lisampole kapa polokelo nako e telele haholo e lebisa ho senyeha ha DNA ea genomic.

Keletso: Li-swabs tsa molomo ka ho khetheha li lokela ho etsoa sampole e ncha, 'me ha ho bohlale ho sebelisa li-swabs tse bolokiloeng bakeng sa ts'ebetso ea ho ntša DNA ea genomic;disampole tsa letheba la madi di lokela ho etsa bonnete ba hore boleng bo tshwaneleha mme nako ya polokelo ha ya lokela ho ba telele haholo.

2. Tšebeliso e fokolang haholo ea lisele e ka 'na ea etsa hore ho se ke ha ntšoa DNA e tsamaisanang le eona.

Keletso: Latela eabuccal litaelo tsa sampling tsa swab bukeng ea ts'ebetso, 'me u hlakole ka makhetlo a mangata kamoo ho ka khonehang e le hore lisele tse lekaneng li ka kopanngoa le sesepa sa molomo bakeng sa ho ntšoa ha DNA ea genomic;bakeng sa ho ntšoa ha letheba la mali, sebaka sa ho seha letheba la mali se ka eketsoa ka nepo.

3. Foregene Protease e bolokiloe ka mokhoa o sa lokelang, e leng se bakang ho fokotseha ha mosebetsi oa eona kapa ho se sebetse.

Keletso: Netefatsa maemo a polokelo eaea Foregene Protease kapa sebakeng sa eona ka Foregene Protease e ncha bakeng sa karabelo ea enzymatic.

4. Ho boloka nako e sa lokelang ea kit kapa nako ea ho boloka nako e telele haholo, ho fella ka ho hlōleha ha likarolo tse ling ka har'a kit.

Keletso: Reka e nchaBuccal swab DNAho behwa ka thoko kit bakeng sa mekhoa e amanang le eona.

5. Buffer WB ha e kenye ethanol e felletseng.

Keletso: Netefatsa hore buffer WB e eketsa molumo o nepahetseng oa ethanol e felletseng.

6. Sehlahisoa ha sea kenyelletsoa ka nepo filiming ea silicone.

Keletso: Eketsa 65°Cmarotholi a futhumetseng pele a futhumala bohareng ba lera la silicone ebe o tlohela mocheso oa kamore bakeng sa metsotso e 5 ho eketsa katleho ea elution.

Low-yield genomic DNA ka thoko

Lisosa tse latelang tse ka hlahang ke tse latelang:

1. Paballo e fosahetseng ea lisampole kapa polokelo nako e telele haholo e lebisa ho senyeha ha DNA ea genomic.

Keletso: Li-swabs tsa molomo li ka khethoa e le tse sa tsoa etsoa sampole, 'me li-swabs tse bolokiloeng ha lia lokela ho sebelisoa bakeng sa ho ntšoa ha DNA ea genomic.

2. Haeba palo ea sampole ea linama e nyane haholo, litaba tsa DNA tse nkiloeng li tla fokotseha.

Keletso: Latela litaelo tsa oral swab tsa sampole bukeng ea ts'ebetso, u hlakole ka makhetlo a mangata kamoo ho ka khonehang e le hore lisele tse lekaneng li ka hokeloa ho oral swab bakeng sa ho ntšoa ha genomic DNA.

3. Foregene Protease e bolokiloe ka mokhoa o sa lokelang, e leng se bakang ho fokotseha ha mosebetsi oa eona kapa ho se sebetse.

Keletso: Netefatsa maemo a polokelo eathe Foregene Protease kapa sebakeng sa eona ka e ncha Foregene Protease bakeng sa karabelo ea enzymatic.

4. Eluent mathata.

Keletso: Sebelisa Buffer EB bakeng sa elution;haeba o sebelisa ddH₂O kapa lintlha tse ling, tiisa hore pH ea eluate e pakeng tsa 7.0-8.5.

5. The eluate ha e eketsoe ka nepo dropwise.

Keletso: Eketsa marotholi a seng makae bohareng ba lera la silicone 'me u siee mocheso oa kamore metsotso e 5 ho eketsa katleho ea elution.

6. Mokelikeli oa elution o bokellana hanyenyane haholo.

Keletso: Sebelisa eluent bakeng sa genomic DNA elution joalo ka ha ho hlokahala litaelong, bonyaneeseng ka tlase ho feta 15μl.

Lbohloeki bo bokae of DNA ea genomicka thoko

Bohloeki bo tlase ba DNA ea genomic bo ka lebisa ho hloleheng kapa liphello tse sa khotsofatseng tsa liteko tse tlaase, tse kang: li-enzyme li ke ke tsa khaoloa li bulehile, PCR ha e khone ho fumana sekhechana sa liphatsa tsa lefutso, joalo-joalo.

Lisosa tse ka bang teng ke tse latelang:

1. Tšilafalo ea Heteroprotein, tšilafalo ea RNA.

Tlhahlobo: Kholomo ea tlhoekiso ha ea ka ea hlatsuoa ho sebelisoa Buffer PW;kholomo ea tlhoekiso ea Buffer PW ha ea ka ea hlatsuoa ho sebelisoa lebelo le nepahetseng la centrifugal.

Keletso: Etsa bonnete ba hore ha ho na pula ho matla a phahametseng tlhaho pele u eketsa ethanol;etsa bonnete ba ho hlatsoa kholomo ea tlhoekiso ho latela litaelo, 'me mohato ona o ke ke oa tloheloa.

2. Tšilafalo ea ion tšilafalo.

Tlhahlobo: Kholomo ea tlhoekiso ea li-buffer WB e siiloe kapa ea hlatsuoa hanngoe feela, e leng se ileng sa baka tšilafalo e setseng ea ionic.

Keletso: Etsa bonnete ba hore u hlatsoa Buffer WB makhetlo a 2 kamoo u laetsoeng ho tlosa li-ion tse setseng ka hohle kamoo ho ka khonehang.

3. Tšilafalo ea enzyme ea RNA.

Analysis: Li-RNA tsa kantle ho naha li kentsoe ho buffer;Ts'ebetso ea ho hlatsoa PW ea Buffer e ne e fosahetse, e bakileng masala a RNase, a ama ts'ebetso ea liteko tsa RNA e tlase, joalo ka mongolo oa in vitro.

Keletso: Foregene series nucleic acidisolalisebelisoa tsa tion li ka tlosa RNA ntle le tlatsetso e eketsehileng ea RNase,joalo buccal Swab / FTA Card DNA Isolation Kithloka't eketsa RNase;etsa bonnete ba hore u latela litaelo tsa kholomo ea tlhoekiso ea ho hlatsuoa ea Buffer PW, 'me mohato ona o ke ke oa tloheloa.

4. Masala a Ethanol.

Tlhahlobo: Buffer WB ha ea ka ea etsa centrifugation e se nang letho ka mor'a ho hlatsoa kholomo ea tlhoekiso.

Keletso: Etsa ts'ebetso e nepahetseng ea tube centrifugation e nepahetseng ho latela litaelo.

5. Tšilafalo e ’ngoe ea litšila.

Tlhahlobo: Lisampole tse bolokiloeng kapa lisampole tse khethehileng ha li etsoe esale pele.

Keletso: Hlophisa sampole esale pele joalokaha u laetsoe.