DirectPCR ke eng?
Theknoloji ea DirectPCR e bolela hore ntle le ho arola le ho hloekisa limolek'hule tsa nucleic acid (ho kenyeletsoa DNA le RNA) ea lisampole tse sa tšoaneng tsa lisele, ke feela lisele le sebopeho sa lisele tse senyehang ke lysis ea protease, nucleic acid e lokolloa ka tharollo ea lysis, 'me tharollo ea lysis e kenngoa ka ho toba.Sistimi ea karabelo ea PCR ke theknoloji ea ho holisa mofuta o lebeletsoeng.
E batla phetoho
Karohano le ho ntšoa ha taba ea nucleic acid
Ho tloha ha ho qaptjoa theknoloji ea PCR lilemong tse 30 tse fetileng, bafuputsi ba 'nile ba tšoenngoa ke ho arohana le ho ntšoa ha nucleic acid.Ho sebelisa lisampole tsa lisele ho etsa karabelo ea PCR ka kotloloho ke toro ea bafuputsi ba bangata.Empa toro ena ha e e-s'o phethahale ka lilemo tse 30.Lebaka ke hore lesela le arohaneng le tla lokolla lisebelisoa tse ngata tse thibelang.
likarolo tsena inhibitory tla ba le phello e matla inhibitory ka karabelo ea PCR, ba bang ba tla etsa hore primer le thempleite se khone ho tlama, ba bang ba na le matla protheine denaturation tshebetso, ho fella ka ho inctivation ea nucleic acid polymerase, 'me ba bang ka ho toba thibela primer le thempleite ho tloha tlama .Tsena ke lintlha tse etsang hore karabelo ea PCR e se ke ea tsoela pele hantle.
Ka tloaelo, batho ka linako tse ling ba sebelisa lisebelisoa tsa PCR ho matlafatsa kutlo, ho hlaka le ho holisa karabelo ea PCR.Leha ho le joalo, ka lebaka la li-inhibitor tse fapaneng tsa PCR tse nang le li-template tsa nucleic acid tse tsoang mehloling e fapaneng, ho hlakile hore tšebeliso ea nako e telele ea li-enhancers ha e na thuso, litšenyehelo li phahame, 'me ts'ebetso e boima.
DirectPCR ea pele
Katleho e ka sehloohong lefatšeng --Two Techniques
Foregene e entse likhatelopele lefapheng lena la theknoloji lefatšeng ka bophara.Theknoloji ea Foregene DirectPCR e na le lintlha tse peli tsa tekheniki, tse nolofalletsang Foregene ho ba pula-maliboho le moetapele oa theknoloji ea DirectPCR.
Taba ea pele, mokhoa oa patented nucleic acid polymerase modification.Forgene e na le mokhoa oa ho fetola nuclease o nang le tokelo ea molao, o lebisitsoeng sebakeng se tlamang ho etsa hore nuclease le template e be matla, ka ts'ebetso e phahameng, katleho e phahameng ea amplification le botsitso ba mocheso.
Taba ea bobeli, foromo ea PCR Mix e ntlafalitsoeng bakeng sa mefuta e fapaneng, lisebelisoa tse ikhethang tsa karabelo, li-optimizer le li-stabilizer, li ntlafatsa haholo khanyetso ea polymerase ho PCR inhibitors le ho netefatsa katleho ea matlafatso.
Ke hantle ka lebaka la likhatelopele tse peli tsa bohlokoa tsa thekenoloji tse boletsoeng ka holimo moo Foregene e hlokometseng DirectPCR ea 'nete lefatšeng ka bophara.Ebang ke li-tissue tse tloaelehileng tsa liphoofolo, maro a 'mele, lisele tsa semela, makhasi kapa malebela a metso, kapa esita le peo ea limela, basebelisi ba ka fihlela ntlafatso e tobileng ea PCR ntle le ho senyeha ha mochine kapa mokhoa o tenang oa ho hloekisa nucleic acid.
Bakeng sa lisebelisoa tsa DirectPCR tsa letoto la limela le letoto la liphoofolo tse seng li thakhotsoe, Foregene a ka bua ka boikhohomoso hore re na le matšoao a pele a ts'ebetso lefatšeng.Nakong e tlang, Foregene e tla tsoela pele ho hlahisa letoto la lihlahisoa tse ntseng li e-na le matšoao a ts'ebetso ea lefats'e (kapa esita le tse ikhethang).
Ho fumana lintlha tse ling, kenya:
http://www.foregene.com/http://www.foreivd.com/
Nako ea poso: Feb-27-2017
