Ho feta halofo ea 2022, tloo 'me u bone hore na ke lihlooho life tse phahameng tse ngotsoeng ke Foeregene!
01
Haeba: 17.694
E hatisitsoe ke: The First Affiliated Hospital, Sun Yat-sen University
Litaba tse ka sehloohong: Esophageal squamous cell carcinoma (ESCC) ke mofuta o ka sehloohong oa mofetše oa 'metso.Ho tseba lipheo tse sebetsang hantle tsa kalafo ho bohlokoa haholo bakeng sa kalafo e betere ea ESCC.Liphuputso tse ling li bontšitse hore phetoho e fosahetseng ea RNA e ka khothaletsa polelo le phetolelo ea li-mRNA tse amanang le lisele tsa mofetše, empa mokhoa oa limolek'hule o ntse o sa hlaka.Phuputso ena e bontšitse hore liprotheine tse rarahaneng tse amanang le tRNA m7G methyltransferase METTL1 le WDR4 li eketsehile haholo ka har'a li-tissue tsa ESCC 'me li kentse letsoho ho se tsebeng hantle ha ESCC.Ho tloha ka nako eo, liphuputso tse amanang le liphatsa tsa lefutso tse amanang le METTL1 le WDR4 ho litoeba tsa mohlala oa ESCC li netefalitse hore ts'ebetso e ntlafetseng ea tRNA m7G methyltransferase e lebisa taolong e holimo ea polelo ea liprotheine tsena tse peli, e leng eona e khothalletsang ho ata ha lisele tsa ESCC.Phuputso ena e bonts'a ts'ebetso ea bohlokoa ea oncogenic ea phetoho e fosahetseng ea tRNA m7G ho ESCC 'me e fana ka maikutlo a hore ho lebisa tlhokomelo ea METTL1 le litsela tsa eona tsa matšoao a tlaase ho ka' na ha e-ba le lipakane tsa phekolo bakeng sa phekolo ea ESCC.
Sehlahisoa se thusang:
Mouse Mohatla Direct PCR Kit Foregene#TP-01333
Sehokelo sa mantlha:
https://www.nature.com/articles/s41467-022-29125-7
02
HAEBA:14.903
E hatisitsoe ke: State Key Laboratory ea Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, Univesithi ea Macau
Litaba tsa mantlha:
Ka nts'etsopele ea theknoloji ea maiketsetso, mRNA e fetohile sehlopha se secha sa meriana ea phekolo.Leha ho le joalo, ho fihlela phepelo e nepahetseng ea li-intracellular tsa lithethefatsi tsa mRNA e ntse e le phephetso ka lebaka la kutlo ea mRNA ho RNases.Boithuto bona bo fana ka morero o bonolo oa ho fana ka li-nanostructures tsa polyphenol-conjugated tse ka fanang ka IL-10 mRNA ka nepo.Ka mor'a ho sebelisa nanostructure ena ho fana ka lithethefatsi tsa mRNA, litekanyetso tsa IL-10 li ne li laoloa ka ho khetheha, li thibela ka mokhoa o hlakileng polelo ea lintlha tsa ho ruruha, ho khothalletsa ho lokisoa ha mucosal, ho sireletsa lisele tsa colonic epithelial ho tloha apoptosis, le ho thibela dextran sodium sulfate-induced acute and chronic inflammatory, ho fana ka liphello tsa bohlokoa tsa lefutso la murine colitis.
Sehlahisoa se thusang:
Phoofolo Kakaretso ea RNA Isolation Kit Foregene#RE-03011
Sehokelo sa mantlha:
03
HAEBA:12.499
E hatisitsoe ke: Laboratori ea Teko ea Moetso oa Mafu a Liphoofolo, Koleche ea Bongaka ba Liphoofolo, Univesithi ea Temo ea Sichuan
Litaba tsa mantlha:
Khatello ea oxidative ea peō ea botona ke ntho e ka sehloohong e lebisang ho hlokeng thari ha banna, kahoo ke khetho e ka khonehang le e sebetsang ea ho theha mefuta ea liphoofolo tsa ho hloka thari ea banna ka ho lokisa tsamaiso ea antioxidant ka mokhoa o reriloeng.Leha ho le joalo, mekhoa ea li-antioxidant e amanang le kokoana-hloko ea banna ha e so hlake.Phuputso ena e netefalitse hore lefutso la LanCL1 ke leloko le ikhethileng la antioxidant liseleng tsa banna, 'me polelo ea eona e fokotsehile kapa e amahanngoa le ho hloka thari ha banna.Litoeba tse tona tse haelloang ke lefutso la LanCL1 li bonts'a tšenyo ea oxidative ea peō ea botona le thari e sa sebetseng hantle.Ts'ebetsong ea ho arola peō ea lisele tsa likokoana-hloko, hang ha tšekamelo ea oxidation e etsahala, transcription factor-specific protein 1 (SP1) e tla laola polelo ea LanCL1 gene, kahoo e thibela mokhoa oa oxidation oa peō.Phuputso ena e bonts'a hore tsela ea SP1-LanCL1 e na le karolo ea bohlokoa taolong ea homeostasis ea testicular le tsoalo ea banna e kenelletseng ke redox balance, 'me e fana ka maikutlo a hore litoeba tse fetotsoeng LanCL1 li na le ts'ebeliso e khahlehang e le mohlala oa liphoofolo oa ho hloka thari ha banna.
Sehlahisoa se Thusang:
Mohatla oa Mouse DNA Mini Kit Foregene#DE-05211
Sehokelo sa mantlha:
http://europepmc.org/article/MED/35469022
Mohatla oa Mohatla Direct PCR Kit
◮ Ha ho hlokahale tlhoekiso ea DNA e jang nako le e turang.
◮ Theko ea sampole e nyane, nka peo e le 'ngoe feela.
◮ Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, ’me ho buuoa ho bonolo.
◮Sistimi e ntlafalitsoeng ea PCR e thusa PCR ho ba le maemo a holimo le mamello e matla ho PCR reaction inhibitors.
Animal Total RNA Isolation Kit
◮Ha ho na lebaka la ho tšoenyeha ka ho senyeha ha RNA.Tsamaiso eohle ke RNase-Free.
◮ Tlosa DNA ka katleho u sebelisa DNA-Cleaning Column.
◮Tlosa DNA ntle le ho eketsa DNase.
◮ E bonolo—mesebetsi eohle e phethoa ka mocheso oa kamore.
◮Ka potlako—opereishene e ka phethoa ka mor’a metsotso e 30.
◮Sireletsehile—ha ho sehlahisoa sa tlhaho se sebelisoang.
◮ Bohloeki bo phahameng—OD260/280≈1.8-2.1.
Mohatla oa Mohatla DNA Mini Kit
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Nako ea poso: Sep-13-2022
