Hot-start Taq enzyme e sebelisoa haholo.Ha ho bapisoa le DNA polymerase e tloaelehileng, enzyme ea Taq e chesang e ka qoba ho holisoa ho hong ho sa tobang le ho theha li-primer dimers, 'me e ka ntlafatsa ka katleho sekhahla sa katleho ea phallo ea liphatsa tsa lefutso.Haholo-holo lefapheng la tlhahlobo ea liphatsa tsa lefutso, enzyme ea Taq e chesang e qalile e khethiloe e le tekanyetso e tlamang indastering, 'me DNA polymerase e tloaelehileng ha ea lokela ho sebelisoa.Joalokaha ho ka bonoa ho tsoa ka holimo, li-enzyme tsa Taq tse chesang li sebelisoa haholo.Hajoale, ho na le mefuta e mengata ea li-enzyme tsa Taq tse qalang 'marakeng oa lapeng, empa ha ho na li-enzyme tse ngata tsa Taq tse nang le boleng bo holimo.Ha re tobane le lihlahisoa tse ngata tse chesang tsa Taq, re lokela ho khetha joang?

1. Khetha enzyme ea Taq e chesang e qalang ka katleho e phahameng ea amplification

Ho sebetsa hantle ha PCR ho amana haufi-ufi le ts'ebetso ea Taq enzyme.Kamora hore ts'ebetso e ntle ea karabelo ea enzyme ea Taq e ntlafatsoe, bokhoni ba ho holisa bo kaholimo ho 95%, 'me mefuta e fapaneng ea matlafatso ea palo ea template ea pele e pharaletseng.Katoloso e khotsofatsang e ka fumaneha ha liphatsa tsa lefutso tse shebiloeng li le tlase, 'me ha ho bonolo ho tšeloa chefo ha palo ea template e le holimo,' me nako ea ho phahamisa maikutlo e le telele.Bakeng sa enzyme ea Taq e nang le ts'ebetso e mpe, le haeba sistimi ea karabelo e ntlafalitsoe ka makhetlo a mangata, matla a ho holisa a ntse a le ka tlase ho 90%, sebopeho sa "S" sa mokoloko oa amplification ha se bonahale, moepa o monyane, 'me mothinya o bataletse.Ha palo ea template e le tlase, e ke ke ea phahamisoa, 'me ha palo ea template e phahame, phello ea amplification ha e ntle.Ka hona, khetho ea li-polymerase tsa DNA tse nang le matla a ho holisa haholo ho bohlokoa katlehong ea PCR le qPCR.

2. Khetha hot-start Taq enzyme e nang le matla a matla a enzyme

Matla a enzymatic a enzyme ea Taq a amana le katleho ea ho holisa.Ka kakaretso, ha matla a enzymatic a enzyme ea Taq e qala ho chesa, nako e telele ea kholo ea PCR e hola, 'mele o bōpehileng joaloka S', o phahamisa boleng ba lets'oao la fluorescence, 'me o loketse haholoanyane ho lemoha multiplex PCR.Li-polymerase tsa Brand DNA tse nang le matla a fokolang a enzymatic hangata li ka ts'ehetsa karabelo ea 2-plex.Ha o etsa karabelo ea 3-plex, curve ea amplification e tlase, boleng ba lets'oao la fluorescence bo tlase, 'me ha ho na lekhalo le tloaelehileng la amplification, kahoo ho thata ho ahlola liphetho.

3. Khetha enzyme e chesang ea Taq e nang le kutloisiso e phahameng

Ka kakaretso, DNA polymerase e na le bokhoni bo phahameng ba ho holisa le ho ba le kutlo e phahameng, empa ho boetse ho na le ho se lumellane.Haeba bongata ba liphatsa tsa lefutso tsa sampole e lokelang ho holisoa bo le tlase, ho khothaletsoa ho lekola kutloisiso ea amplification ea Taq enzyme.Mokhoa o tloaelehileng oa ho lemoha ke oa ho hlapolla ha 10-fold kapa 5-fold gradient fragment ea target gene plasmid, ho etsa ponelopele ea PCR sebakeng se tlase sa dilution, ebe u khetha enzyme ea Taq e chesang e nang le kutlo e phahameng ea ho lemoha.

Ho ka bonoa ho tsoa ka holimo hore bafuputsi ba lokela ho khetha ho latela litlhoko tsa bona tsa liteko le maemo a lichelete.Ho molemo ho etsa teko ea matlafatso ea gradient dilution ho bona bokhoni ba ho holisa le kutlo ea enzyme ea Taq e qalang.

Mohlala oa Foregene's Taq DNA Polymerase:

HS Taq DNA Polymerase

Tlhaloso

Foreasy HS Taq DNA Polymerase ke DNA polymerase e hlahisoang ho baktheria ea Escherichia coli ea boenjiniere ka theknoloji ea ho kopanya liphatsa tsa lefutso.Enzyme e kopantsoe le "buffffer" e ikhethang, e etsang hore sehlahisoa se hanyetsane haholo 'me se tsamaisane,' me se ka sebelisa ka ho toba sampole ea lysate (Foregene Lysis system) e le template bakeng sa karabelo ea ho lemoha.

Kopo

PCR ea boleng bo phahameng le tlhahlobo ea PCR ea palo ea litempele tse hloekisitsoeng le litempele tse sa hloekisoang.

Taolo ea Boleng

1.Ha ho ts'ebetso ea nuclease ea kantle e fumanoeng

Mokhoa oa 2.PCR oa ho lemoha DNA e setseng ea genomic ntle le moeti

3.E ka matlafatsa liphatsa tsa lefutso tsa kopi e le 'ngoe ho Genome ea motho

4. Boloka ka mocheso oa kamore bakeng sa beke, mesebetsi e sa bonahaleng e fetoha

Lintlha tsa sehlahisoa: https://www.foreivd.com/foreasy-hs-taq-dna-polymerase-product/