lncRNA likarolo:

1. Li-lncRNA hangata li telele, li na le polelo e matla le mekhoa e fapaneng ea ho kopanya nakong ea phapang;

2. Ha ho bapisoa le liphatsa tsa lefutso tsa likhoutu, lncRNA hangata e tlase;

3. Li-lncRNA tse ngata li na le tlhaloso e hlakileng ea nakoana le ea sebaka ka mokhoa oa ho khetholla ha lisele le nts'etsopele;

4. Ho na le litšobotsi tse hlalosang lihlahala le mafu a mang;

5. Libaka tse nyenyane tsa lncRNA li fapane;

6. Tatelano e tlase ka paballo, empa mosebetsi o na le tekanyo e itseng ea paballo joalo-joalo.

LncRNA reverse transcript solution:

Rea tseba hore lncRNA e na le sebopeho sa boemo bo holimo liseleng.Hangata RNA e joalo e na le mekhoa e rarahaneng e kang li-loops kapa li-hairpins.Haeba sebopeho sa bobeli sa lncRNA se ke ke sa rarolloa ka nepo, phetisetso ea morao-rao e batla e se atlehe kapa e se atlehe ha o etsa RT-qPCR.Bothata ba ts'ebetso e tlase.

Ka kakaretso, ho na le lintlha tse tharo tsa bohlokoa tseo re ka fumanang litempele tsa cDNA tsa boleng bo holimo:

Boleng ba thempleite ea RNA, li-primer tsa transcript le reverse transcriptase, le reverse transcriptase ke eona ntlha ea mantlha.RNA e hloekisitsoeng ka li-reagents tsa RNA tse atisang ho sebelisoa hangata li na le masala a joala le letsoai la guanidine, tse nang le liphello tse matla tsa ho thibela li-reverse transcriptases;haeba thempereichara e nepahetseng bakeng sa reverse transcriptase reaction e le tlase, e ke ke ea lumellana le RNA.Ho buloa hoa sebopeho sa maemo.

Hajoale, thempereichara e loketseng bakeng sa reverse transcriptase e sebelisoang lilaboratoring tse ngata hangata ke 42°C, ’me mocheso o phahameng o ka fihla ho 50°C.Ha e le hantle, ho buloa ha sebopeho sa RNA sa boemo bo holimo ho hloka mocheso o phahameng.

Mocheso oa ho kopanya cDNA ea pele ea Fortune Biotech 2 × RT EasyTM Mix e phahame ho 50 ℃-65 ℃, e leng se loketseng ho manolla ka ho feletseng sebopeho sa boemo bo phahameng ba lncRNA, 'me nako ea ts'ebetso e khutšoanyane.

Ntle le moo, sehlahisoa se boetse se na le matla a matla a ho thibela tšitiso bakeng sa RNA e nang le joala bo setseng le letsoai la guanidine, le ka fihlelang litlhoko tsa lilaboratori ka botlalo ho fihlela katleho e phahameng ea ho fetolela.

Pele Strand cDNA Synthesis Kit

Nomoro ea lintlha: RT-01011 (makhetlo a 25)

Likaroloana:

1. Thermal botsitso: 65 ℃ e ntse e na le ts'ebetso e ntle ea ho fetolela, e thusang ho bula sebopeho se rarahaneng sa bobeli.

2. Mamello: motsoako oa cDNA o ntse o ka etsoa ka katleho ho litempele tsa RNA ka bohloeki bo bobe (masala a letsoai a ethanol le guanidine)

3. Ka potlako: ts'ebetso e bonolo, motsoako oa pele oa cDNA oa strand o ka phethoa nakong ea metsotso e 25-35.

Lisebelisoa tse tlase: PCR, qPCR, RACE, ho ngola li-chip, kaho ea laeborari, joalo-joalo.

Ntle le moo, FJ Biotech e fana ka tharollo e felletseng ea RT-PCR ho fihlela litlhoko tsa mefuta e fapaneng ea bareki, ho kenyelletsa le lisebelisoa tse latelang:

First Strand cDNA Synthesis Kit (Bakeng sa qPCR)

Nomoro ea thepa: RT-01021 (makhetlo a 50)

Likaroloana:

1. Bonolo: Ho kentsoe li-primers tsa reverse transcription ho nolofatsa tšebetso

2. Thermal botsitso: 65 ℃ e ntse e na le ts'ebetso e ntle ea ho fetolela, e thusang ho bula sebopeho se rarahaneng sa bobeli.

3. Mamello: motsoako oa cDNA o ntse o ka etsoa ka katleho ho litempele tsa RNA ka bohloeki bo bobe

4. Sensitivity: e loketse lisampole tsa RNA tse fokolang haholo

Ts'ebeliso e tlase: E fanoe ka ho khetheha bakeng sa qPCR e latelang

Mohato o le mong oa RT-PCR kit

Nomoro ea lintlha: RT-02011 (100 makhetlo)

Likaroloana:

1. Thermal botsitso: 65 ℃ e ntse e na le ts'ebetso e ntle ea ho fetolela, e thusang ho bula sebopeho se rarahaneng sa bobeli.

2. Mamello: motsoako oa cDNA o ntse o ka etsoa ka katleho ho litempele tsa RNA ka bohloeki bo bobe

3. Sets'oants'o: PCR MIX e nang le lisebelisoa e ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.

Lisebelisoa tse tlase: cDNA cloning, tlhahlobo ea liphatsa tsa lefutso le liteko tse ling tsa limolek'hule

Mehato e 'meli ea RT-PCR kit

Nomoro ea lintho: RT-02021 (100 makhetlo)

Likaroloana:

1. E bonolo ebile e potlakile: karabelo ea tube e le 'ngoe

2. Thermal botsitso: 65 ℃ e ntse e na le ts'ebetso e ntle ea ho fetolela, e thusang ho bula sebopeho se rarahaneng sa bobeli.

3. Sets'oants'o: Foregene Taq e kenyellelitsoeng e ntlafatsa ka nepo matla a ho holisa le ho nepahala ha karabelo.

Lisebelisoa tse tlase: liteko tsa limolek'hule tse kang tlhahlobo ea lefutso

Mohato o le mong oa RT-qPCR kit-SYBR Green I

Nomoro ea lintlha: RT-02111 (100 makhetlo)

Likaroloana:

1. E bonolo: karabelo ea tube e le 'ngoe

2. Kutloisiso e phahameng: e khona ho bona litempele tsa kopi tse tlase

3. Ho lumellana: ho loketse lisebelisoa tse ngata tsa fluorescence quantitative

Lisebelisoa tse tlase: ho lemoha lisampole tsa RNA tse kang lisele le livaerase

Mohato o le mong oa RT-qPCR kit-Taqman

Nomoro ea lintho: RT-02131 (100 makhetlo)

Likaroloana:

1. Ho potlaka: lemoha lefutso la sepheo

2. Kutloisiso e phahameng: e khona ho bona litempele tsa kopi tse tlase

3. Ho lumellana: ho loketse lisebelisoa tse ngata tsa fluorescence quantitative

Lisebelisoa tse tlase: ho lemoha lisampole tsa RNA tse kang lisele le livaerase
Forge Bioreverse Transcription Kit e ka u thusa ho rarolla bothata ba ho fetolela ka morao.Ka nako e ts'oanang, e sebelisana le Forge Bio RNA Extraction Kit (e ka tlosang masala a DNA ka katleho) ho u fa tharollo e feletseng ea liteko tsa RNA.