U tseba hakae

Mabapi le Nho tlosoa ha ucleic acid

Tšimoloho le qetello ea hloekisitsoeng nucleic acid

Ntho e 'ngoe le e' ngoe e thata qalong, nucleic acid e hloekisitsoeng ke eona e ngata ka ho fetisisa

Ho qala liteko tsa limolek'hule, bakeng sa liteko tse latelang

Katleho kapa ho hloleha ho na le tšusumetso e kholo.

Trace/ultra-trace UV spectrophotometer e ratoa ke bafuputsi ba bangata ba mahlale hobane e khona ho bona mahloriso a nucleic acid le masala a liprotheine le li-ion tsa letsoai habonolo, kapele le ka moruo.

Joalo ka ha bohle re tseba, A260 e bolela hore nucleic acid e monya boleng ba OD, A280 e bolela boleng ba protheine ea OD, A230 e bolela ho monya mahloriso a letsoai OD boleng, kahoo A260 e khona ho fetola mahloriso a nucleic acid, A260/280 e bolela masala a protheine, A260/230 e bolela masala a letsoai a ion, empa sena ke masala a mangata a thehiloeng ho liphuputso.tloaelehileng” ho lumela hore e ka hlalosa bohloeki ba DNA le RNA hotekanyo e itseng.Hase eona feela tekanyetso ea ho khetholla lihlahisoa tsa nucleic acid le bohloeki, e sebelisoa feela e le boitsebiso, 'me ha se "tekanyetso ea khauta".

Buka ea Thermo Fisher ND-2000 e totobatsa ho tšepahala ha liphetho tsa liteko

Lebaka ke hore liphetho tse fumanoeng ka ho sebelisa micro/ultramicro UV spectrophotometer li bonts'a feela chai le bohloeki ba nucleic acid ho tloha ka lehlakoreng, 'me ho na le lintlha tse ngata tse susumetsang (optical path, volume volume, sample concentration, pH value, ion tse ling tse amang tekanyo ea ho monya, joalo-joalo) , boleng ba OD bo lekantsoeng ha se hakaalo hore bo nepahetse.Ka nako e ts'oanang, ka lebaka la khaello ea lintlha tse hlakileng tsa boleng ba ho monya, DNA e nang le mela e le 'ngoe, DNA e nang le likarolo tse peli, RNA, dNTPs, le liprotheine tse ling kaofela li na le litlhōrō tsa ho monya ho 260nm wavelength,' me mahloriso a khethiloeng ke A260 feela ha se DNA kapa RNA ea sebele.Ho tsepamisa maikutlo, le botšepehi ba eona le ho theoloa ha eona ho ke ke ha ahloloa.

Joale mokhoa oa ho ahlola boleng ba acid ea rona e hloekisitsoeng ea nucleic?Ntle le ho sebelisa micro/ultramicro UV spectrophotometer bakeng sa ho lemoha, mekhoa e kang agarose gel electrophoresis e boetse e hlokoa ho bonts'a bohloeki le botšepehi ba nucleic acid, le ho bontša ho tsepamisa mohopolo ho isa bohōleng bo itseng.Ka tsela ena, tlhahiso ea nucleic acid le bohloeki bo fumanoeng ho tsoa liphethong tsa mekhoa e fapaneng lia tšepahala.

Papiso ea chate ea liteko

Genomic DNA ea lisele tsa H1299 e ile ea ntšoa ho sebelisoa lik'hamphani tsa A's DNA Extraction, 'me tšilafalo e kholo ea litšila e ile ea bonoa, e leng se ileng sa fella ka litekanyetso tse phahameng tsa OD.

(Boleng ba tekanyo ea OD le electropherogram e lumellanang)

Tlhahlobo ea index

Na ho na le "tekanyetso ea khauta" bakeng sa tlhahiso ea nucleic acid le tlhahlobo ea boleng?

Ho ea kamoo re tsebang kateng, ha ho na mokhoa o bonolo, o potlakileng le o theko e tlaase.Ebang ke spectrophotometer, gel electrophoresis, kapa mass spectrometry, kaofela ha tsona li bonahatsa mahloriso le bohloeki ba nucleic acid ka tharollo ho tsoa likarolong tse fapaneng, 'me li hloka ho ahloloa ka ho bua ka e mong.

Molemo ka ho fetisisa evaluation index ke kamehlats'ebeliso ea tlhahlobo ea morao-rao.Ha e ame ts'ebetso ea reverse transcript le PCR amplification.Ho fumana lihlahisoa tse ka tšeptjoang tsa amplification le litekanyetso tsa Ct, le ho fumana tatellano e feletseng ka ho latellana ke katleho ea nucleic acid extract.

Ho akaretsa, maikutlo a khopolo-taba-feela a lokela ho qholotsoa ke maikutlo a "ho sebelisa liphetho tse ntle tsa liteko tse tlase joalo ka litekanyetso tse ntle tsa ho hula"!

Li-kits tse khothalelitsoeng tsa Foregene DNA RNA ho fumana bohloeki bo phahameng ba DNA/RNA:

https://www.foreivd.com/reagent/dna-isolation-series/

https://www.foreivd.com/reagent/rna-isolation-series/