Selelekela:

Limolek'hule tsa RNA li ka sebelisana haholo le limolek'hule tse ling tsa likokoana-hloko, tse kang protheine, DNA le RNA, 'me kahoo li teng liseleng le linthong tse phelang ka mokhoa oa macromolecular complexes.Har'a tsona, RNA-protein complexes ke ho ba teng le ho sebetsa ha RNA E 'ngoe ea mefuta ea bohlokoa ea.RNA ho hula fatše ke mokhoa oa khale oa ho hlahloba le ho khetholla limolek'hule tsa RNA, haholo-holo liprotheine tse sebelisanang tsa limolek'hule tsa lncRNA, 'me li amoheloa ke bongata ba bafuputsi le likoranta tsa machaba tse nang le matla a thuto.Kajeno FORREGENE e tla arolelana le uena molao-motheo oa liteko le ts'ebetso ea tekheniki ea ho theola RNA, mohlomong u ka e sebelisa.

1. Molao-motheo oa liteko oa RNA ho theola theknoloji:

In vitro transcription and synthesis of biotin-labeled target lncRNA kapa lncRNA fragments (ho kenyeletsoa likhechana tsa RNA tse sebelisoang e le li-taolo tse ntle le tse mpe), le ho e kenya ka li-extracts tsa lisele;sebelisa li-avidin-anchored magnetic beads ho bokella RNA-protein kapa RNA-RNA complexes, Ka mor'a ho hlatsoa ka ho feletseng, ho rarahana hoa eluted.Haeba u lemoha molek'hule ea sepheo sa protheine e sebelisanang le lncRNA, etsa protheine electrophoresis ho sehlahisoa sa RNA-hull down, fumana protheine e lebisitsoeng ka matheba a silevera, 'me u sehe gel bakeng sa spectrometry ea boima;hape etsa netefatso ea blot ea bophirima ka kotloloho sehlahisoa se hulang;ha e lemohuoa Bakeng sa limolek'hule tse shebiloeng tsa RNA tse sebelisanang le lncRNA, lihlahisoa tsa RNA-hull down li kenngoa ka har'a RNA 'me ho etsoa tlhahlobo e khethehileng ea qRT-PCR.

2. RNA e theola tšebetso ea liteko tsa theknoloji:

3. Ts'ebeletso ea tekheniki ea RNA e theola FOREGENE:

Setso sa sele

Tokisetso ea kakaretso ea sele / nuclear / plasma protheine extract

RNA e theola liteko

SDS-PAGE electrophoresis, letheba la silevera

Mass spectrometry le liteko tsa WB

Tlaleho ea liteko

4. Nyeoe ea teko

Palo e latelang e bontša liphetho tsa FJ Bio-Bio's RNA hula fatše le SDS-PAGE electrophoresis discovery, moo motsu o mofubelu o tšoaeang sehlopha sa phapang:

Sehlopha sa lipatlisiso sa Moprofesa Song Xu oa Univesithi ea Sichuan se bokelletse lilemo tse 15 tsa ho bokella nako e telele lefapheng la lipatlisiso tsa lncRNA.Sehlopha sa lipatlisiso sa Foregene Bio le Moprofesa Song Xu se ile sa sebelisana 'moho ho qala theknoloji ea RNA ea ho theola lncRNA, e tla u fa sephetho se khotsofatsang bakeng sa liteko tsa hau.