Ho na le mefuta e 'meli e meholo ea likotsi lilaboratoring tsa PCR: likotsi tsa biosafety le likotsi tsa tšilafalo ea nucleic acid.Ea pele e senya batho le tikoloho, 'me ea morao-rao e ama liphello tsa liteko tsa PCR.Sengoliloeng sena se bua ka lintlha tsa tlhahlobo ea kotsi ea laboratori ea PCR le maemo a ts'oanang a kotsi a tlisoang ho uena.

01 Karohano ea laboratori ea PCR

1. Laeborari ea tlhahlobo ea baeloji ea limolek'hule

Ho latela litlhoko tsa Athikele ea 1.1 ea Litekanyetso tsa Motheo bakeng sa Lilaboratori tsa Teko tsa Clinical Gene Amplification Testing, lilaboratori tsa PCR ka kakaretso li na le libaka tse 'ne: sebaka sa polokelo ea li-reagent le sebaka sa ho lokisa, sebaka sa ho lokisa sa mohlala, sebaka sa ho holisa, le sebaka sa tlhahlobo ea sehlahisoa.Haeba mokhoa oa PCR oa nako ea sebele oa fluorescent o sebelisoa, sebaka sa amplification le sebaka sa tlhahlobo se ka kopanngoa sebakeng se le seng;haeba PCR analyzer e feletseng e sebelisoa, sebaka sa ho lokisetsa mohlala, sebaka sa amplification le sebaka sa tlhahlobo se ka kopanngoa sebakeng se le seng.

Buka ea "Workbook for New Coronavirus Nucleic Acid Testing in Medical Institutions (Trial Version 2)" e bolela hore ha e le hantle, lilaboratori tse etsang tlhahlobo e ncha ea "coronavirus nucleic acid" li lokela ho theha libaka tse latelang: sebaka sa polokelo ea lisebelisoa le sebaka sa ho lokisa, sebaka sa ho lokisetsa mohlala, sebaka sa ho holisa le ho hlahloba lihlahisoa.Libaka tsena tse tharo li lokela ho ikemela ka ho feletseng sebakeng sa 'mele,' me ho ke ke ha e-ba le puisano e tobileng le moea.

2. Kamore ea ho lokisetsa mohlala

Le hoja disampole li ka lokisoa habonolo sebakeng sa ho lokisetsa mohlala, kamore e khethehileng ea ho lokisetsa mohlala e ntse e hlokahala ha ho sebetsanoa le lisampole tse rarahaneng le palo e kholo ea lisampole.Kamore ea ho lokisetsa mohlala e na le kotsi e kholo ea tšireletso ea likokoana-hloko le tšilafalo ea nucleic acid.

3. Kamore ea ho hloekisa litšila

Phekolo ea litšila e sa nepahalang le eona e tla tlisa likotsi tse kholo tsa ts'oaetso ea biosafety le nucleic acid ka laboratoring.Ka hona, kamore ea ho hloekisa litšila e hloka ho hlahlojoa khafetsa.

02 Libaka tsa ho beha leihlo kotsi lilaboratoring tsa PCR

Lilaboratori tse arohaneng li arotsoe ka phaposi ea ho itokisa mohlala, sebaka sa polokelo ea lisebelisoa le sebaka sa ho lokisa, sebaka sa ho lokisetsoa sa mohlala, sebaka sa ho holisa le ho hlahloba lihlahisoa, le phaposi ea ho hloekisa litšila.

Ho latela mofuta oa sebaka sa sampole, se arotsoe ka bokaholimo, sesebelisoa, sampole, tlhahlobo ea tikoloho le pipette.

Boemo ba kotsi bo tloha tlase ho ea holimo ho tloha ho naleli★ ho ea ho linaleli tse tharo★★★.

1. Kamore ea ho lokisetsa mohlala:

E sebedisetswa ho ngodisa, ho lokisa le ho kenya disampole tshebetsong, mme kotsi ya polokeho ya baeloji ke e phahameng ka ho fetisisa.Hobane lisampole ha li ntšitsoe le ho holisoa, ntle le li-pipette tse atisang ho kopana le lisampole, kotsi ea tšilafalo ea nucleic acid likarolong tse ling e tlaase.

1-4 Ho etsa sampole libakeng tsa tlhahlobo

5-8 Sampling sebakeng sa ho beha leihlo

9-12 monitoring point sampling

1. Sebaka sa polokelo le tokiso ea reagent:

E sebelisetsoa ho lokisa li-reagents tsa polokelo, ho fana ka li-reagents le ho lokisa motsoako oa amplification reaction, hammoho le ho boloka le ho lokisa lintho tse sebelisoang tse kang li-tubes tsa centrifuge le malebela a pipette.Ha ho na kamano e tobileng le lisampole ebile ha ho na nucleic acid e ntle sebakeng sena, kahoo kotsi ea biosafety le kotsi ea tšilafalo ea nucleic acid e tlase.

13-16 Ho etsa sampole libakeng tsa tlhahlobo

17-22 Sampling at monitoring
3. Mohlala oa lintlha tsa ho lokisetsa

E sebelisetsoa ho bula moqomo oa phetisetso, ho tima mohlala (ha ho hlokahala), ho ntša nucleic acid le ho e eketsa ho amplification reaction tube, joalo-joalo Sebaka sena se ka 'na sa kenyelletsa ho sebetsa le ho buloa ha lisampole, kotsi ea ts'ireletso ea likokoana-hloko e phahame,' me ho ntšoa ha nucleic acid ho etsoa, ​​​​'me kotsi ea tšilafalo ea nucleic acid e bohareng ho ea holimo.

29 Ho etsa sampole libakeng tsa tlhahlobo

4. Sebaka sa ntlafatso le tlhahlobo ea lihlahisoa:

E sebelisoa bakeng sa ho matlafatsa nucleic acid.Sebaka sena ha se kenyelletse tšebetso ea lisampole, 'me kotsi ea polokeho ea likokoana-hloko e tlase.The nucleic acid amplification haholo-holo sebakeng sena, 'me kotsi ea ho silafala ha nucleic acid e phahame ka ho fetisisa.

38 Ho etsa sampole libakeng tsa tlhahlobo
5. Kamore ea ho hloekisa litšila:

E sebelisetsoa ho sebetsana le khatello e phahameng ea lisampole.Likotsi tsa polokeho ea baeloji tse amehang tšebetsong ea lisampole sebakeng sena li batla li phahame.Lihlahisoa tsa nucleic acid amplification li khothaletsoa hore li tšoaroe joalo ka litšila tsa bongaka.Khatello e phahameng ha e khothalletsoe, 'me kotsi ea tšilafalo ea nucleic acid e tlaase.

43-44 Ho etsa sampole libakeng tsa tlhahlobo

03 Phethahatsa

Lekhetlong lena re thathamisitse lintlha tse 44 tsa tlhahlobo.Ho hakanngoa hore batho ba bangata ba tlameha ho botsa, na ba hloka ho etsa lintlha tse ngata hakaale?E, etsa tsohle!Ke fana ka tlhahiso ea hore u qale ho etsa tlhahlobo ea kotsi ea laboratori ea hau, e ka etsoang ho latela kotsi ho tloha holimo ho ea tlase, u ka boela ua beha leihlo mofuta o tšoanang oa lisampole hammoho, kapa u ka etsa moralo oa sampole bakeng sa ho beha leihlo khafetsa.Ka bokhutšoanyane, laboratori ka 'ngoe e ka iketsetsa moralo oa eona oa ts'ebetsong ho latela maemo a eona.Kotsi e kholo ea ho etsa liteko lilaboratori ke ho iphapanyetsa likotsi.