Mali a DNA Midi Kit (1-5ml) Mali a Isolation Midi Kits for DNA from Blood
Tlhaloso ea Kit:
Ka potlako hloekisa DNA ea boleng bo holimo ho tsoa maling a anticoagulated (1-5ml).
◮Ha ho tšilafalo ea RNase:DNA-Feela Column e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho thibela laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
◮Lebelo le potlakileng:Foregene Protease e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso ea 20-80.
◮E loketse:The centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4 ° C mocheso o tlase oa centrifugation kapa ethanol precipitation ea DNA.
◮Tšireletseho:Ha ho hlokahale ho ntšoa ha reagent ea organic.
◮Boleng bo phahameng:DNA e nkiloeng e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlase haholo, tse ka fihlelang litlhoko tsa liteko tse fapaneng.
◮Sistimi ea Micro-elution:E ka eketsa bongata ba DNA ea genomic, e leng bonolo ho lemoha kapa ho etsa liteko.
Tlhaloso
Mohlala oa mali o na le palo e kholo ea lisele tse khubelu tsa mali le lisele tsa platelet, e leng se etsang hore ts'ebetso ea mali e be thata haholoanyane.Leha ho le joalo, DNA ea mali ea genomic e nkiloeng ka mokhoa o akaretsang ha e na bohloeki bo phahameng 'me litaba li tlaase haholo.Setsi sena se sebelisa Foregene Protease Plus e ncha le lisebelisoa tse ikhethang tsa BL1 le BL2, tse khonang ho sila mali a anticoagulated ka botlalo.coagulationdisampole ka nako e khuts'oane, ka hona ho qoba ho senyeha ha DNA ho isa tekanyong e kholo le ho fumana palo e kholo ka ho fetisisa ea DNA ea genomic.Sistimi e potlakileng ea ts'ebetso ea mali le ts'ebetso e bonolo ea li-spin e nolofatsa haholo ho ntšoa ha liphatsa tsa lefutso tsa mali, e le hore DNA ea boleng bo holimo, e hloekileng e ka fumanoa nakong ea metsotso e 40.
DNA-feela silica gel membrane e sebelisitsoeng mokokotlong oa kit ena ke ntho e ncha e ikhethang ea k'hamphani, e hlahisang DNA ka katleho le ka ho khetheha, 'me e ka tlosa RNA, liprotheine tse sa hloekang, li-ion le metsoako e meng ea lintho tse phelang liseleng ho isa tekanyong e kholo ka ho fetisisa.Likaroloana tse fumanoeng tsa DNA li kholo, li na le bohloeki bo phahameng, boleng bo tsitsitseng le bo ka tšeptjoang, 'me boholo ba ho jara kholomo ea spin ke 80 μg DNA.DNA e fumanoeng e ka sebelisoa bakeng sa liteko tsa baeloji ea limolek'hule tse kang thibelo ea tšilo ea lijo, PCR, hybridization ea Southern, kaho ea laebrari joalo-joalo.Thepa e ka sebetsa ho fihlela ho 5ml ea mali ka nako ho fumana palo e lekaneng ea DNA ea genomic e hloekileng haholo.
Litlhaloso
50 Preps, 100 Preps, 250 Preps
Likarolo tsa Kit
| Buffer BL1 |
| Buffer BL2* |
| Buffer DC |
| Buffer PW* |
| Sekhahla sa WB1 |
| Buffer EB |
| Foregene Protease Plus |
| DNA-Feela Kholomo |
| Litaelo |
Likarolo le melemo
-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.
-Lebelo le potlakileng: Foregene Protease Plus e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso e 40.
-E bonolo: centrifugation e etsoa ka mocheso oa kamore, 'me ha ho hlokahale 4°C centrifugation ea mocheso o tlase kapa pula ea ethanol ea DNA.
-Tshireletso: Ha ho hlokahale ho ntšoa ha reagent ea organic.
-Boleng bo phahameng: DNA e nkiloeng ea genomic e na le likhechana tse kholo, ha e na RNA, ha e na RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse sa tšoaneng.
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisa DNA ea genomic maling a foreshe kapa a leqhoa a anticoagulated.
Mokhoa oa ho sebetsa
Storage le Shelf bophelo
-Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25).℃);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8℃.
Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.
-Forene Protease Plus tharollo e na le mokhoa o ikhethang, o sebetsang ha o bolokoa mocheso oa kamore nako e telele (likhoeli tse 3), mosebetsi oa eona le botsitso ba eona li tla ba molemo ha li bolokiloe ho 4 ° C, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20.℃.
