China Plant leaf Direct PCR Plus kit (ntle le Sampling Tools) Protocol Direct PCR ho tloha ho Moetsi oa Lintho tsa Semela le Mofani |Foregene

Lekhasi la semela Direct PCR Plus kit (ntle le lisebelisoa tsa sampole) Protocol Direct PCR ho tsoa ho Plant Material.

Lekhasi la semela Direct PCR Plus kit (ntle le lisebelisoa tsa ho etsa sampole) Protocol Direct PCR ho tsoa ho Plant Material Image Featured

Tlhaloso ea Kit:

Ka ho toba sebelisa polysaccharide le polyphenol lekhasi lysate e le template bakeng sa PCR amplification, ntle le ho ntšoa ka thōko ho DNA, ka potlako le ka kutloisiso e phahameng.

◮Ha ho tlhoekiso ea DNA e jang nako le e turang (Nako, mosebetsi le litšenyehelo li bolokiloe!)

◮Boitsebiso bo fokolang

◮E bonolo - sampole e ka ba tlas'a karabelo ea PCR kapa karabelo ea lysis ntle le ho seha kapa ho sila

◮2 X Mix-ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi

◮Tokiso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa ka metsotso e 50

◮High throughput-lysis reaction e ka phethoa ka poleiti ea PCR ea seliba tse 96

Re romelle lengolo-tsoibila Khoasolla e le PDF

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso

Sehlahisoa sena se sebelisa mokhoa o ikhethileng oa lysis buffer ho lisa makhasi a semela.Lysate e ka sebelisoa e le template ntle le tlhoekiso.Kamora ho kenyelletsa li-primers, PCR Mix ea kit ena e ka sebelisoa bakeng sa ho holisa.

Litlhaloso

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Likarolo tsa Kit

Karolo ea I	Buffer PS1
	Buffer P2
	6×DNA Loading Buffer
Karolo ea II	Buffer PS2
Karolo ea II	2× Lekhasi la PCR le bonolo^TMKopanya

Likarolo le melemo

■ Ha ho tlhoekiso ea DNA e jang nako le e theko e boima
■ Boitsebiso bo fokolang
■ E bonolo-sampole e ka ba tlas'a PCR reaction kapa lysis reaction ntle le ho seha kapa ho sila
■ 2 × Mix, ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
■ Tokisetso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa bonyane ka metsotso e 50.
■ Boitšoaro bo phahameng ba throughput-lysis bo ka phethoa ka poleiti ea PCR ea seliba sa 96