China China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit Moetsi le Mofani |Foregene

China Supplier China Factory Magnetic Bead Acid Nucleic Test Kit Rt-PCR Kit

Tlhaloso ea Kit:

Setsi sena se sebelisa mokhoa o ikhethileng oa lysis buffer system ho lokolla kapele DNA ea genomic ho liqoaha le lithane tse ling tsa tlhapi ea metsi a hloekileng, mapheoana a mohatla kapa lisampole tsa mahe a tlhapi bakeng sa karabelo ea PCR, ka hona e loketse haholo tlhahlobo e kholo ea liphatsa tsa lefutso.Mokhoa oa ho lokolla DNA ea genomic ho tswa ho lysis buffer e phethoa ka hare ho metsotso e 10-30 ho 65 ° C.Ha ho na mekhoa e meng e kang ho tlosoa ha protheine le RNA e hlokahalang, 'me DNA ea trace e lokolotsoeng e ka sebelisoa e le template bakeng sa karabelo ea PCR.

2 × PCR EasyTM Mix (UNG) e sebelisa dUTP ho e-na le dTTP motheong oa 2 × PCR EasyTM Mix, mme e eketsa enzyme ea UNG (Uracil-N-glycosylase) e ka senyang template e nang le dUTP ka nako e le 'ngoe.Pele ho karabelo ea PCR, enzyme ea UNG e sebelisoa ho nyenyefatsa sehlahisoa sa PCR se nang le uracil.Enzyme ea UNG e ke ke ea e-ba le phello leha e le efe ho template e se nang uracil, ka tsela eo e netefatsa ho nepahala le ho nepahala ha amplification le ho thibela monyetla oa tlhahlobo e kholo ea liphatsa tsa lefutso.Tšoaetso ea lihlahisoa tsa PCR e etsahetse.

D-Taq DNA polymerase ke DNA polymerase e entsoeng ka ho khetheha ke Foregene bakeng sa karabelo e tobileng ea PCR.D-Taq DNA polymerase e na le mamello e matla ho mefuta e fapaneng ea li-inhibitors tsa PCR, 'me e khona ho holisa DNA ka mokhoa o nepahetseng lits'ebetsong tse fapaneng tse rarahaneng tsa karabelo, mme lebelo la amplification le ka fihla ho 2Kb/min, e loketseng ka ho khetheha bakeng sa karabelo ea Direct PCR.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Re na le sehlopha se sebetsang hantle haholo ho sebetsana le lipotso tse tsoang ho bareki.Sepheo sa rona ke "100% khotsofalo ea bareki ka boleng ba sehlahisoa sa rona, theko & tšebeletso ea sehlopha sa rona" 'me re natefeloa ke botumo bo botle har'a bareki.Ka lifeme tse ngata, re ka fana ka mefuta e mengata e fapaneng ea China Supplier China Factory Magnetic Bead Nucleic Test Kit Rt-PCR Kit, feme ea rona e ile ea hola kapele ka boholo le botumo ka lebaka la boinehelo ba eona bo felletseng ba tlhahiso ea boleng bo holimo, litheko tse ngata tsa tharollo le lits'ebeletso tse ntle tsa bareki.
Re na le sehlopha se sebetsang hantle haholo ho sebetsana le lipotso tse tsoang ho bareki.Sepheo sa rona ke "100% khotsofalo ea bareki ka boleng ba sehlahisoa sa rona, theko & tšebeletso ea sehlopha sa rona" 'me re natefeloa ke botumo bo botle har'a bareki.Ka lifeme tse ngata, re ka fana ka mefuta e mengata eaChina Extraction Kit, Nucleic Acid Extract Kit, Khampani ea rona e lula e tsepamisitse maikutlo ho nts'etsopele ea 'maraka oa machaba.Hona joale re na le bareki ba bangata Russia, linaha tsa Europe, USA, linaha tsa Middle East le linaha tsa Afrika.Kamehla re latela hore boleng ke motheo ha tšebeletso e le tiiso ea ho kopana le bareki bohle.

Litlhaloso

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Likarolo tsa Kit

Karolo ea I	Buffer FP
	Foregene Protease
	6× DNA Loading Buffer
Karolo ea II	2 × PCR e bonolo^TMMotsoako(UNG)
Litaelo

Likarolo le melemo

■ Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.

■ Tlhokahalo ea sampole e nyane, joalo ka ha 1mg tlhapi ea caudal fin kapa mahe a 10 a tlhapi a ka lekoa.

■ Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, ’me ho buuoa ho bonolo.

■ Sistimi ea PCR e khahlanong le tšilafalo ea 2× PCR EasyTM Mix (UNG), e ka felisang ka katleho tšilafalo e bakoang ke lihlahisoa tsa PCR le ho netefatsa ho nepahala le ho nepahala ha amplification.

■ Sistimi e ntlafalitsoeng ea PCR e etsa hore PCR e be le maemo a holimo le mamello e matla ho PCR reaction inhibitors.

Litekanyetso tsa kit

Kopo: Litlhapi tsa Zebra le litlhapi tse ling tsa metsi a hloekileng.

DNA e lokollotsoeng ho tsoa ho sampole lysis: e sebelisoa feela joalo ka template ea PCR.

Thepa e ka sebelisoa bakeng sa merero e latelang: ho tsebahatsa transgenosis, genotyping ea liphoofolo, joalo-joalo.

Storage le Shelf bophelo

Karolo ea I ea kit ena e lokela ho bolokoa ho 2-8 ℃.

Reagent Buffer FP e ka bolokoa likhoeli tse 12 tlas'a maemo a omileng;e ka bolokoa ho 2-8 ℃ bakeng sa polokelo e telele.

v Reagent Foregene Protease e na le foromo e ikhethang.Ho netefatsa ts'ebetso ea eona le botsitso, ka kopo e boloke ho 4 ℃ bakeng sa likhoeli tse 12.

vReagent 6 × DNA Loading Buffer e ka bolokoa ho 4 ℃ kapa -20 ℃ nako e telele.

Karolo ea II ea kit ena e lokela ho bolokoa ho -20 ℃.

vReagent 2 × PCR EasyTM Mix(UNG), haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa pele ho matsatsi a 10).

Re na le sehlopha se sebetsang hantle haholo ho sebetsana le lipotso tse tsoang ho bareki.Sepheo sa rona ke "100% khotsofalo ea bareki ka boleng ba sehlahisoa sa rona, theko & tšebeletso ea sehlopha sa rona" 'me re natefeloa ke botumo bo botle har'a bareki.Ka lifeme tse ngata, re ka fana ka mefuta e mengata e fapaneng ea China Supplier China Factory Magnetic Bead Nucleic Test Kit Rt-PCR Kit, feme ea rona e ile ea hola kapele ka boholo le botumo ka lebaka la boinehelo ba eona bo felletseng ba tlhahiso ea boleng bo holimo, litheko tse ngata tsa tharollo le lits'ebeletso tse ntle tsa bareki.
China SupplierChina Extraction Kit, Nucleic Acid Extract Kit, Khampani ea rona e lula e tsepamisitse maikutlo ho nts'etsopele ea 'maraka oa machaba.Hona joale re na le bareki ba bangata Russia, linaha tsa Europe, USA, linaha tsa Middle East le linaha tsa Afrika.Kamehla re latela hore boleng ke motheo ha tšebeletso e le tiiso ea ho kopana le bareki bohle.

E fetileng: Setsebi sa Machaena sa China Nucleic Acid Extraction Reagent (Serum/ Plasma le Tharollo ea Puncture) , Rna Extraction Test Kits

E 'ngoe: Theko ea fektheri China Lebitso la Kakaretso: Nucleic Acid Extraction Kit

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