Lihlahisoa tsa rona li khetholloa ka ho pharaletseng ebile li ka tšeptjoa ke bareki 'me li tla khotsofatsa litakatso tse tsoelang pele tsa moruo le tsa sechaba bakeng sa China Techstar Hipure Viral Nucleic Aicd Isolation Kit (lifaha tsa makenete, mokhoa oa matsoho / o ikemetseng), Joaloka sehlopha se nang le phihlelo re boetse re amohela litaelo tse hlophisitsoeng.Sepheo se seholo sa k'hamphani ea rona ke ho aha mohopolo o khotsofatsang bakeng sa bareki bohle, le ho theha kamano ea nako e telele ea katleho ea khoebo.
Lihlahisoa tsa rona li khetholloa ka ho pharaletseng ebile li ka tšeptjoa ke bareki 'me li tla khotsofatsa kamehla litakatso tsa moruo le tsa sechaba bakeng saChina Nucleic Acid Extraction Kit, DNA/Rna Extraction Kit, Re lumela ho theha likamano tse ntle tsa bareki le tšebelisano e ntle bakeng sa khoebo.Tšebelisano e haufi le bareki ba rona e re thusitse ho theha liketane tse matla tsa phepelo le ho fumana melemo.Lihlahisoa tsa rona li re fumane ka kamohelo e atileng le khotsofalo ea bareki ba rona ba bohlokoa lefatšeng ka bophara.

Litlhaloso tsa Kits

50 Preps, 200 Preps

Setsi sena se sebelisa "spin column le formula" e entsoeng ke k'hamphani ea rona, e ka ntšang RNA e hloekileng le ea boleng bo phahameng ka ho fetisisa ho tsoa ho mefuta e sa tšoaneng ea liphoofolo tse nang le bokhoni bo phahameng.E fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo le ho bapatsa DNA ea genomic ho tloha ho supernatant le lysate ea lisele, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.

Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, ​​e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.

Likarolo tsa Kit:

Likarolo le melemo

■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.

Lihlahisoa tsa lihlahisoa

■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×10⁶
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl

Phallo ea mosebetsi

Setšoantšo

Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar

Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer RL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli ea 1 ka mor'a ho eketsa β- mercaptoethanol (ho ikhethela).

Lihlahisoa tsa rona li khetholloa ka ho pharaletseng ebile li ka tšeptjoa ke bareki 'me li tla khotsofatsa litakatso tse tsoelang pele tsa moruo le tsa sechaba bakeng sa China Techstar Hipure Viral Nucleic Aicd Isolation Kit (lifaha tsa makenete, mokhoa oa matsoho / o ikemetseng), Joaloka sehlopha se nang le phihlelo re boetse re amohela litaelo tse hlophisitsoeng.Sepheo se seholo sa k'hamphani ea rona ke ho aha mohopolo o khotsofatsang bakeng sa bareki bohle, le ho theha kamano ea nako e telele ea katleho ea khoebo.
China wholesaleChina Nucleic Acid Extraction Kit, DNA/Rna Extraction Kit, Re lumela ho theha likamano tse ntle tsa bareki le tšebelisano e ntle bakeng sa khoebo.Tšebelisano e haufi le bareki ba rona e re thusitse ho theha liketane tse matla tsa phepelo le ho fumana melemo.Lihlahisoa tsa rona li re fumane ka kamohelo e atileng le khotsofalo ea bareki ba rona ba bohlokoa lefatšeng ka bophara.