Ka mokhoa o ts'eptjoang oa boleng bo botle, rekoto e ntle le ts'ebeletso e phethahetseng ea bareki, letoto la litharollo tse hlahisoang ke khoebo ea rona li romelloa linaheng tse ngata le libaka bakeng sa Tharollo ea Ts'ireletso ea Ts'oaetso ea Ts'oaetso ea Ts'ebetso ea China ka Rnase Inhibitors, 'me re ka thusa ho batla lihlahisoa life kapa life tsa litlhoko tsa bareki.Etsa bonnete ba hore u fana ka litšebeletso tse ntle ka ho fetisisa, boleng bo botle ka ho fetisisa, thomello e potlakileng.
Ka mokhoa o ts'eptjoang oa boleng bo botle, rekoto e ntle le ts'ebeletso e phethahetseng ea bareki, letoto la litharollo tse hlahisoang ke khoebo ea rona li romelloa linaheng tse ngata le libakengTs'ireletso ea Virus ea China e nang le Rnase Inhibitors le Reagent e sa sebetseng ea ho boloka kokoana, Bakeng sa mang kapa mang ea thahasellang thepa leha e le efe ea rona hang ka mor'a hore u shebe lethathamo la lihlahisoa tsa rona, ka kopo ikutloe u lokolohile ho ikopanya le rona bakeng sa lipotso.O khona ho re romella mangolo-tsoibila le ho ikopanya le rona bakeng sa lipuisano mme re tla u araba kapele kamoo re ka khonang.Haeba ho le bonolo, o ka fumana aterese ea rona sebakeng sa rona sa marang-rang mme oa tla khoebong ea rona ho fumana lintlha tse ling tsa tharollo ea rona ka bouena.Haesale re le malala-a-laotsoe ho aha likamano tse atolositsoeng le tse tsitsitseng tsa tšebelisano-'moho le bareki leha e le bafe ba ka bang teng mafapheng a amanang.

Litlhaloso

5ml, 10ml, 40ml

PCR Hero (With Dye) Extraction kit e kenyelletsa moloko o mocha o ikhethang oa Foregene oa Taq-Foregene Taq Hero, dNTP e ntlafalitsoeng, MgCl2, buffer reaction, PCR reaction enhancer, optimizer le stabilizer.Sistimi ea 2 × PCR Hero TM Mix (dae) e na le mamello e phahameng ho li-inhibitors tsa PCR ho feta sistimi e tloaelehileng ea PCR Mix, 'me e ka sebetsana ka katleho le ntlafatso ea PCR ea li-template tse fapaneng tse rarahaneng.Sistimi e ikhethang ea karabelo le ts'ebetso e phahameng ea Taq Hero li etsa hore karabelo ea PCR e be le bokhoni bo phahameng ba ho holisa, boits'oaro le kutlo.

Likarolo tsa Kit

Likarolo le melemo

■ Botšepehi bo phahameng: makhetlo a 6 ho feta a enzyme e tloaelehileng ea Taq;

■ Lebelo la ho hōlisa ka potlako : lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;

■ Ho ikamahanya le maemo ho hoholo ha template: E khona ho holisa li-template tse fapaneng tse rarahaneng tsa DNA ka holimoLitaba tsa GC;

■ Bokhoni bo phahameng ba ho holisa: lipotoloho tse tlase tsa matlafatso ho feta enzyme e tloaelehileng ea Taq;

■ Ho mamella tikoloho ho matlafetse: ho behoa ho 37 ° C ka beke, ho boloka ho feta 90% mosebetsi;

■ E na le mosebetsi oa 5′ →3′ DNA polymerase le 5′→3′ exonuclease mosebetsi, ntle le 3′ →5′ exonuclease mosebetsi.

■ Sekoti se kopantsoe pele le Electrophoresis Loading Buffer, ka mor'a hore PCR e phethoe, sampuli e ka kenngoa ka ho toba bakeng sa electrophoresis;ts'ebetso e bonolo ebile e bonolo.

Sesebelisoa sa Kit

Kholiso ea PCR ea likhechana tsa DNA, ho ngola DNA, tatellano ea DNA, PCR le mohatla oa A

Storage le Shelf bophelo

E ka bolokoa nako e telele ho -20 ° C;Ka mokhoa o ts'eptjoang oa boleng bo botle, rekoto e monate le ts'ebeletso e phethahetseng ea bareki, letoto la litharollo tse hlahisoang ke khoebo ea rona li romelloa linaheng tse ngata le libaka tsa China Professional Inactivated Efficient Virus Preservation Solution le Rnase Inhibitors, 'me re ka thusa ho batla lihlahisoa leha e le life tsa litlhoko tsa bareki.Etsa bonnete ba hore u fana ka litšebeletso tse ntle ka ho fetisisa, boleng bo botle ka ho fetisisa, thomello e potlakileng.
Setsebi sa SechaenaTs'ireletso ea Virus ea China e nang le Rnase Inhibitors le Reagent e sa sebetseng ea ho boloka kokoana, Bakeng sa mang kapa mang ea thahasellang thepa leha e le efe ea rona hang ka mor'a hore u shebe lethathamo la lihlahisoa tsa rona, ka kopo ikutloe u lokolohile ho ikopanya le rona bakeng sa lipotso.O khona ho re romella mangolo-tsoibila le ho ikopanya le rona bakeng sa lipuisano mme re tla u araba kapele kamoo re ka khonang.Haeba ho le bonolo, o ka fumana aterese ea rona sebakeng sa rona sa marang-rang mme oa tla khoebong ea rona ho fumana lintlha tse ling tsa tharollo ea rona ka bouena.Haesale re le malala-a-laotsoe ho aha likamano tse atolositsoeng le tse tsitsitseng tsa tšebelisano-'moho le bareki leha e le bafe ba ka bang teng mafapheng a amanang.