Basebeletsi ba rona hangata ba moeeng oa "ntlafatso e tsoelang pele le bokhabane", 'me ha re ntse re sebelisa thepa ea boleng bo holimo, boleng bo holimo le lits'ebeletso tse holimo ka mor'a thekiso, re leka ho fumana tumelo ea moreki e mong le e mong bakeng sa thekiso ea theko e phahameng ka ho fetesisa ea China Method Rna Nucleic Acid Extraction Kit le Purification Kit, Ho isa bareki ka lisebelisoa le lik'hamphani tse ntle haholo, mme sepheo sa rona khafetsa ke mochini o mocha oa khoebo.Re lebeletse tšebelisano ea hau esale pele.
Basebetsi ba rona hangata ba na le moea oa "ntlafatso e tsoelang pele le bokhabane", 'me ha re ntse re sebelisa thepa ea boleng bo holimo, boleng bo holimo le lits'ebeletso tse holimo ka mor'a thekiso, re leka ho fumana tumelo ea moreki e mong le e mong bakeng saChina Nucleic Acid Extraction, Mokhoa oa Magnetic Bead, Re beha tsamaiso e thata ea ho laola boleng.Re na le leano la ho khutlisa le ho fapanyetsana, 'me u ka fapanyetsana nakong ea matsatsi a 7 ka mor'a hore u fumane li-wigs haeba li le seteisheneng se secha' me re fana ka litšebeletso mahala bakeng sa tharollo ea rona.U lokela ho ikutloa u lokolohile ho ikopanya le rona bakeng sa tlhaiso-leseling e batsi 'me re tla u fa lenane la litheko tsa tlholisano ka nako eo.

Litlhaloso tsa Kits

50 Preps, 200 Preps

Setsi sena se sebelisa "spin column le formula" e entsoeng ke k'hamphani ea rona, e ka ntšang RNA e hloekileng le ea boleng bo phahameng ka ho fetisisa ho tsoa ho mefuta e sa tšoaneng ea liphoofolo tse nang le bokhoni bo phahameng.E fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo le ho bapatsa DNA ea genomic ho tloha ho supernatant le lysate ea lisele, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.

Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, ​​e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.

Likarolo tsa Kit:

Likarolo le melemo

■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.

Lihlahisoa tsa lihlahisoa

■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×10⁶
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl

Phallo ea mosebetsi

Setšoantšo

Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar

Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer RL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli ea 1 ka mor'a ho eketsa β- mercaptoethanol (ho ikhethela).

Basebeletsi ba rona hangata ba moeeng oa "ntlafatso e tsoelang pele le bokhabane", 'me ha re ntse re sebelisa thepa ea boleng bo holimo, boleng bo holimo le lits'ebeletso tse holimo ka mor'a thekiso, re leka ho fumana tumelo ea moreki e mong le e mong bakeng sa thekiso ea theko e phahameng ka ho fetesisa ea China Method Rna Nucleic Acid Extraction Kit le Purification Kit, Ho isa bareki ka lisebelisoa le lik'hamphani tse ntle haholo, mme sepheo sa rona khafetsa ke mochini o mocha oa khoebo.Re lebeletse tšebelisano ea hau esale pele.
Theolelo ka kakaretsoChina Nucleic Acid Extraction, Mokhoa oa Magnetic Bead, Re beha tsamaiso e thata ea ho laola boleng.Re na le leano la ho khutlisa le ho fapanyetsana, 'me u ka fapanyetsana nakong ea matsatsi a 7 ka mor'a hore u fumane li-wigs haeba li le seteisheneng se secha' me re fana ka litšebeletso mahala bakeng sa tharollo ea rona.U lokela ho ikutloa u lokolohile ho ikopanya le rona bakeng sa tlhaiso-leseling e batsi 'me re tla u fa lenane la litheko tsa tlholisano ka nako eo.