Re ka lula re khotsofatsa bareki ba rona ba hlomphuoang ka boleng ba rona bo botle ba boleng bo holimo, theko e ntle le tšehetso e ntle ka lebaka la hore re bile litsebi tse eketsehileng le ho sebetsa ka thata ho feta 'me re e etsa ka tsela e theko e tlaase bakeng sa boleng bo holimo China bo sebetsang hantle le bo tsitsitseng Lyophilized Dspathtm 4X One-Step Multiplex Master Mix, Etsa bonnete ba hore u ikutloa u lokolohile ho bua le rona bakeng sa mokhatlo.'me re lumela hore re tla arolelana boiphihlelo bo nepahetseng ba khoebo le barekisi bohle ba rona.
Re ka lula re khotsofatsa bareki ba rona ba hlomphuoang ka boleng ba rona bo botle ba boleng bo holimo, theko e ntle ea theko le tšehetso e ntle ka lebaka la hore re bile litsebi tse eketsehileng le ho sebetsa ka thata ho feta mme re e etsa ka tsela e baballang chelete e ngata.China Taq DNA Polymerase, Qpcr, Khamphani ea rona e ntse e tsoela pele ho sebeletsa bareki ka boleng bo holimo, theko ea tlholisano le ho fana ka thepa ka nako.Re amohela ka tieo metsoalle e tsoang lefats'eng lohle ho sebelisana le rona le ho holisa khoebo ea rona.Haeba u thahasella lihlahisoa tsa rona, u lokela ho ikutloa u lokolohile ho ikopanya le rona.Re ka thabela ho u fa lintlha tse ling.

Litlhaloso

5ml, 10ml, 40ml

PCR Hero (With Dye) Extraction kit e kenyelletsa moloko o mocha o ikhethang oa Foregene oa Taq-Foregene Taq Hero, dNTP e ntlafalitsoeng, MgCl2, buffer reaction, PCR reaction enhancer, optimizer le stabilizer.Sistimi ea 2 × PCR Hero TM Mix (dae) e na le mamello e phahameng ho li-inhibitors tsa PCR ho feta sistimi e tloaelehileng ea PCR Mix, 'me e ka sebetsana ka katleho le ntlafatso ea PCR ea li-template tse fapaneng tse rarahaneng.Sistimi e ikhethang ea karabelo le ts'ebetso e phahameng ea Taq Hero li etsa hore karabelo ea PCR e be le bokhoni bo phahameng ba ho holisa, boits'oaro le kutlo.

Likarolo tsa Kit

Likarolo le melemo

■ Botšepehi bo phahameng: makhetlo a 6 ho feta a enzyme e tloaelehileng ea Taq;

■ Lebelo la ho hōlisa ka potlako : lebelo la amplification ke 5-10sec/kb, e leng makhetlo a 6-12 a enzyme e tloaelehileng ea Taq;

■ Ho ikamahanya le maemo ho hoholo ha template: E khona ho holisa li-template tse fapaneng tse rarahaneng tsa DNA ka holimoLitaba tsa GC;

■ Bokhoni bo phahameng ba ho holisa: lipotoloho tse tlase tsa matlafatso ho feta enzyme e tloaelehileng ea Taq;

■ Ho mamella tikoloho ho matlafetse: ho behoa ho 37 ° C ka beke, ho boloka ho feta 90% mosebetsi;

■ E na le mosebetsi oa 5′ →3′ DNA polymerase le 5′→3′ exonuclease mosebetsi, ntle le 3′ →5′ exonuclease mosebetsi.

■ Sekoti se kopantsoe pele le Electrophoresis Loading Buffer, ka mor'a hore PCR e phethoe, sampuli e ka kenngoa ka ho toba bakeng sa electrophoresis;ts'ebetso e bonolo ebile e bonolo.

Sesebelisoa sa Kit

Kholiso ea PCR ea likhechana tsa DNA, ho ngola DNA, tatellano ea DNA, PCR le mohatla oa A

Storage le Shelf bophelo

E ka bolokoa nako e telele ho -20 ° C;bakeng sa tšebeliso ea khafetsa, e ka bolokoa ho 4 ° C ka nako e khutšoanyane. Re ka lula re khotsofatsa bareki ba rona ba hlomphehang ka boleng ba rona bo botle ba boleng bo phahameng, theko e ntle ea theko le tšehetso e ntle ka lebaka la hore re 'nile ra ba setsebi se eketsehileng le ho sebetsa ka thata ho feta' me re se etsa ka mokhoa o theko e tlaase bakeng sa boleng bo botle ba Chaena bo sebetsang ka ho fetisisa le bo tsitsitseng Lyophilized Dspathtm'me re lumela hore re tla arolelana boiphihlelo bo nepahetseng ba khoebo le barekisi bohle ba rona.
Boleng bo kgabaneChina Taq DNA Polymerase, Qpcr, Khamphani ea rona e ntse e tsoela pele ho sebeletsa bareki ka boleng bo holimo, theko ea tlholisano le ho fana ka thepa ka nako.Re amohela ka tieo metsoalle e tsoang lefats'eng lohle ho sebelisana le rona le ho holisa khoebo ea rona.Haeba u thahasella lihlahisoa tsa rona, u lokela ho ikutloa u lokolohile ho ikopanya le rona.Re ka thabela ho u fa lintlha tse ling.