Re boetse re sebetsa ka thata ho matlafatsa tsamaiso ea lintho le mokhoa oa QC ho etsa bonnete ba hore re ka boloka phaello e kholo ha re ntse re le khoebong ea tlholisano e matla bakeng sa Factory For China Techstar Magnetic Bead Method Swab Sample Nucleic Acid Extraction Kit Rna Isolation Reagent DNA Purification Kit, Ho bona ho lumela!Re amohela ka tieo litebello tse ncha tsa kantle ho naha ho theha litšebelisano tsa lik'hamphani hape re lebelletse ho kopanya litšebelisano le bareki bohle ba khale ba thehile.
Re boetse re sebetsa ka thata ho matlafatsa taolo ea lintho le mokhoa oa QC ho netefatsa hore re ka boloka phaello e kholo ha re ntse re le khoebong e nang le tlholisano e matla bakeng saChina Nucleic Acid Reagent, DNA/Rna Extraction Kit, Khamphani ea rona ke morekisi oa machaba oa thepa ea mofuta ona.Re fana ka khetho e makatsang ea litharollo tsa boleng bo holimo.Sepheo sa rona ke ho u thabisa ka pokello ea rona e ikhethang ea lintho tse hlokolosi ha re ntse re fana ka boleng le tšebeletso e babatsehang.Sepheo sa rona se bonolo: Ho fana ka lihlahisoa le tšebeletso tse molemo ka ho fetisisa ho bareki ba rona ka theko e tlaase ka ho fetisisa.

Litlhaloso

50 Preps, bakeng sa tlhoekiso ea DNA ea genomic maling a felletseng<1mlmadi

Buffer DC: E hlahisa lisele tse khubelu tsa mali maling.

Buffer BL1: Fana ka mohlala oa tikoloho ea tšilo ea enzymatic.

Foregene Protease Plus: Mesampole ea mali e sila ka Enzymatic tikolohong ea Buffer BL1.

Buffer BL2: Etsa hore Foregene Protease Plus e sebetse 'me u fane ka tikoloho ea ho kenya DNA.

Buffer PW: Tlosa litšila tse kang protheine le RNA ho DNA.

Buffer WB1: Tlosa li-ion tse setseng tsa letsoai ho DNA.

Buffer EB: Elute DNA ka lera la tlhoekiso.

DNA-Feela Kholomo: Ka ho khetheha e adsorbs genomic DNA ka lysate.

Likarolo tsa Kit

Likarolo le melemo

-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.

-Lebelo le potlakileng: Foregene Protease Plus e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso e 40.

-Bonolo: The centrifugation e etsoa mocheso oa kamore, 4 ° C centrifugation kapa ethanol precipitation ea DNA ha e hlokehe.

-Tshireletso: Ha ho qotsulo ea organic reagent e sebelisoang.

-Boleng bo phahameng: Likaroloana tse nkiloeng tsa genomic DNA li kholo, ha ho na RNA, ha ho RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse fapa-fapaneng.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisa DNA ea genomic maling a foreshe kapa a leqhoa a anticoagulated.

Mokhoa oa ho sebetsa

Storage le Shelf bophelo

Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.

Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.

Tharollo ea Foregene Protease Plus e na le foromo e ikhethang, e sebetsang ha e bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);e tla ba e sebetsang haholoanyane le e tsitsitseng ha e bolokiloe ho 4 ° C, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20 ° C. Re boetse re ikhethela ho matlafatsa tsamaiso ea lintho le mokhoa oa QC ho etsa bonnete ba hore re ka boloka phaello e kholo ha re ntse re le khoebong e mabifi ea tlhōlisano bakeng sa Factory For China Techstar Magnetic Bead Exmplection DNA Requipment DNA Pursod DNA Exploration Kinetic Recipent DNA. , Ho bona ho lumela!Re amohela ka tieo litebello tse ncha tsa kantle ho naha ho theha litšebelisano tsa lik'hamphani hape re lebelletse ho kopanya litšebelisano le bareki bohle ba khale ba thehile.
Fektheri Bakeng saChina Nucleic Acid Reagent, DNA/Rna Extraction Kit, Khamphani ea rona ke morekisi oa machaba oa thepa ea mofuta ona.Re fana ka khetho e makatsang ea litharollo tsa boleng bo holimo.Sepheo sa rona ke ho u thabisa ka pokello ea rona e ikhethang ea lintho tse hlokolosi ha re ntse re fana ka boleng le tšebeletso e babatsehang.Sepheo sa rona se bonolo: Ho fana ka lihlahisoa le tšebeletso tse molemo ka ho fetisisa ho bareki ba rona ka theko e tlaase ka ho fetisisa.