Re sebelisa mokhoa o felletseng oa tsamaiso ea boleng bo botle ba mahlale, boleng bo botle haholo le tumelo e phahameng, re hapile boemo bo botle mme ra lula taeo ena bakeng sa theko e tlase ea feme China Long Taq DNA Polymerase, Long PCR, Re amohela ka tieo baeti bohle ho hlophisa likamano tsa khampani le rona mabapi le motheo oa likarolo tse ntle tse lumellanang.O lokela ho iteanya le rona hona joale.U ka fumana karabo ea rona ea litsebi nakong ea lihora tse 8 tse 'maloa.
Re sebelisa tsamaiso e felletseng ea boleng bo botle ba mahlale, boleng bo botle haholo le tumelo e phahameng, re hapa maemo a matle mme ra nka taeo ena bakeng saPhatlalatso ea PCR ea Chaena, Qpcr, Khamphani ea rona e akaretsa sebaka sa lisekoere-mithara tse 20,000.Re na le basebetsi ba fetang 200, sehlopha sa litsebi tsa litsebi, phihlelo ea lilemo tse 15, mosebetsi o tsoileng matsoho, boleng bo tsitsitseng le bo ka tšeptjoang, theko ea tlhōlisano le bokhoni bo lekaneng ba tlhahiso, ke kamoo re matlafatsang bareki ba rona kateng.Haeba u na le lipotso, ka kopo u se ke ua qeaqea ho iteanya le rona.

Litlhaloso

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Likarolo tsa Kit

Likarolo le melemo

■ Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.

■ Tlhokahalo ea sampole e nyane, joalo ka ha 1mg tlhapi ea caudal fin kapa mahe a 10 a tlhapi a ka lekoa.

■ Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, ’me ho buuoa ho bonolo.

■ Sistimi e ntlafalitsoeng ea PCR e etsa hore PCR e be le maemo a holimo le mamello e matla ho PCR reaction inhibitors.

Litekanyetso tsa kit

Kopo: Litlhapi tsa Zebra le litlhapi tse ling tsa metsi a hloekileng.

DNA e lokollotsoeng ho tsoa ho sampole lysis: e sebelisoa feela joalo ka template ea PCR.

Thepa e ka sebelisoa bakeng sa merero e latelang: ho tsebahatsa transgenosis, genotyping ea liphoofolo, joalo-joalo.

Phallo ea mosebetsi

Storage le Shelf bophelo

Karolo ea I ea kit ena e lokela ho bolokoa ho 2-8 ℃.

Reagent Buffer FP e ka bolokoa likhoeli tse 12 tlas'a maemo a omileng;e ka bolokoa ho 2-8 ℃ bakeng sa polokelo e telele.

v Reagent Foregene Protease e na le foromo e ikhethang.Ho netefatsa ts'ebetso ea eona le botsitso, ka kopo e boloke ho 4 ℃ bakeng sa likhoeli tse 12.

vReagent 6 × DNA Loading Buffer e ka bolokoa ho 4 ℃ kapa -20 ℃ nako e telele.

Karolo ea II ea kit ena e lokela ho bolokoa ho -20 ℃.

vReagent 2 × PCR EasyTM Mix, haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4 ℃ bakeng sa polokelo ea nako e khutšoanyane (sebelisa ka hare ho matsatsi a 10) . Ho sebelisa tsamaiso e feletseng ea saense ea boleng bo botle, boleng bo botle haholo le tumelo e phahameng, re hapa boemo bo botle 'me re nkile taeo ena bakeng sa theko e tlaase ea fektheri China Long Taq DNA Polymerase, Long PCR, Re ka hlophisa likamano tse ntle tsa khampani le rona ka botšepehi.O lokela ho iteanya le rona hona joale.U ka fumana karabo ea rona ea litsebi nakong ea lihora tse 8 tse 'maloa.
theko e tlase ea femePhatlalatso ea PCR ea Chaena, Qpcr, Khamphani ea rona e akaretsa sebaka sa lisekoere-mithara tse 20,000.Re na le basebetsi ba fetang 200, sehlopha sa litsebi tsa litsebi, phihlelo ea lilemo tse 15, mosebetsi o tsoileng matsoho, boleng bo tsitsitseng le bo ka tšeptjoang, theko ea tlhōlisano le bokhoni bo lekaneng ba tlhahiso, ke kamoo re matlafatsang bareki ba rona kateng.Haeba u na le lipotso, ka kopo u se ke ua qeaqea ho iteanya le rona.