Re ts'ehetsa bareki ba rona ka lihlahisoa tsa boleng bo holimo le k'hamphani ea boemo bo holimo.Ho ba moetsi ea hloahloa lekaleng lena, re na le boiphihlelo bo matla ba ho sebetsa ho hlahiseng le ho tsamaisa Feme e etsang China Li-Removal Removal Reagents tsa China tse Non-Chefo le tse sa Senyeng bakeng sa Tlosa Tšilafalo, Amohela potso efe kapa efe k'hamphaning ea rona.Re tla thabela ho theha likamano tsa botsoalle tsa khoebo le uena!
Re ts'ehetsa bareki ba rona ka lihlahisoa tsa boleng bo holimo le k'hamphani ea boemo bo holimo.Ho ba moetsi ea hloahloa lefapheng lena, re na le boiphihlelo bo monate ba ho sebetsa ba ho hlahisa le ho laolaChina Nucleic Acid Remover, Rnase le Dnase Scavenger, Theko e ntle ke eng?Re fa bareki ka theko ea fektheri.Boemong ba boleng bo botle, ts'ebetso e hloka ho eloa hloko le ho boloka phaello e tlase e phetseng hantle.Ho tlisa kapele ke eng?Re etsa tlhahiso ho latela litlhoko tsa bareki.Le hoja nako ea ho fana e itšetlehile ka bongata ba taelo le ho rarahana ha eona, re ntse re leka ho fana ka lintho ka nako.Re tšepa ka tieo hore re ka ba le kamano ea nako e telele ea khoebo.

Litlhaloso

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Likarolo tsa Kit

Likarolo le melemo

■ Ha ho hlokahale tlhoekiso ea DNA e jang nako le e theko e boima.

■ Tlhokahalo ea sampole e nyane, joalo ka ha 1mg tlhapi ea caudal fin kapa mahe a 10 a tlhapi a ka lekoa.

■ Ha ho hlokahale liphekolo tse khethehileng tse kang ho sila le ho sila, ’me ho buuoa ho bonolo.

■ Sistimi ea PCR e khahlanong le tšilafalo ea 2× PCR EasyTM Mix (UNG), e ka felisang ka katleho tšilafalo e bakoang ke lihlahisoa tsa PCR le ho netefatsa ho nepahala le ho nepahala ha amplification.

■ Sistimi e ntlafalitsoeng ea PCR e etsa hore PCR e be le maemo a holimo le mamello e matla ho PCR reaction inhibitors.

Litekanyetso tsa kit

Kopo: Litlhapi tsa Zebra le litlhapi tse ling tsa metsi a hloekileng.

DNA e lokollotsoeng ho tsoa ho sampole lysis: e sebelisoa feela joalo ka template ea PCR.

Thepa e ka sebelisoa bakeng sa merero e latelang: ho tsebahatsa transgenosis, genotyping ea liphoofolo, joalo-joalo.

Storage le Shelf bophelo

Karolo ea I ea kit ena e lokela ho bolokoa ho 2-8 ℃.

Reagent Buffer FP e ka bolokoa likhoeli tse 12 tlas'a maemo a omileng;e ka bolokoa ho 2-8 ℃ bakeng sa polokelo e telele.

v Reagent Foregene Protease e na le foromo e ikhethang.Ho netefatsa ts'ebetso ea eona le botsitso, ka kopo e boloke ho 4 ℃ bakeng sa likhoeli tse 12.

vReagent 6 × DNA Loading Buffer e ka bolokoa ho 4 ℃ kapa -20 ℃ nako e telele.

Karolo ea II ea kit ena e lokela ho bolokoa ho -20 ℃.

vReagent 2×PCR EasyTM Mix(UNG), haeba e sebelisoa khafetsa, e ka boela ea bolokoa ho 4℃ bakeng sa polokelo ea nako e khutšoanyane (e sebelisoang nakong ea matsatsi a 10). Re tšehetsa bareki ba rona ka lihlahisoa tse ntle tsa boleng ba premium le k'hamphani ea boemo bo holimo.Ho ba moetsi ea hloahloa lekaleng lena, re na le boiphihlelo bo matla ba ho sebetsa ba ho hlahisa le ho tsamaisa Feme e etsang China Li-Removal Removal Reagents tsa China tse Non-Chefo le tse sa Senyeng bakeng sa Tlosa Tšilafalo, Amohela potso efe kapa efe k'hamphaning ea rona.Re tla thabela ho theha likamano tsa botsoalle tsa khoebo le uena!
Ho etsa fektheriChina Nucleic Acid Remover, Rnase le Dnase Scavenger, Theko e ntle ke eng?Re fa bareki ka theko ea fektheri.Boemong ba boleng bo botle, ts'ebetso e hloka ho eloa hloko le ho boloka phaello e tlase e phetseng hantle.Ho tlisa kapele ke eng?Re etsa tlhahiso ho latela litlhoko tsa bareki.Le hoja nako ea ho fana e itšetlehile ka bongata ba taelo le ho rarahana ha eona, re ntse re leka ho fana ka lintho ka nako.Re tšepa ka tieo hore re ka ba le kamano ea nako e telele ea khoebo.