Re lumela hore tšebelisano-'moho e telele e bakiloe ke boleng ba boleng bo holimo, lits'ebeletso tse ekelitsoeng, boiphihlelo bo atlehileng le boikopanyo ba feme bakeng sa Virus Magnetic Bead Method Rna-DNA Nucleic Acid 96 Sample Extraction Purification Tube Kit, Re na le maikutlo a hore basebetsi ba chesehang, ba hloahloa le ba koetlisitsoeng hantle ba ka theha likamano tse ntle tsa khoebo le uena kapele.Etsa bonnete ba hore o ikutloa o lokolohile ka botlalo ho ikopanya le rona bakeng sa lintlha tse ling.
Re lumela hore tšebelisano-'moho e telele e bakiloe ke boleng ba boleng bo holimo, litšebeletso tse ekelitsoeng, boiphihlelo bo atlehileng le ho ikopanya le motho ka mongChina Nucleic Acid Extraction Kit (rna-DNA) le 96 Nucleic Acid, Ka ho tsitlallela taolong ea boleng ba boleng bo holimo le thuso ea bareki, joale re thehile qeto ea rona ea ho fa bareki ba rona ho qala ka ho fumana chelete le ka mor'a boiphihlelo ba lits'ebeletso.Ho boloka likamano tsa botsoalle tse teng le bareki ba rona, leha ho le joalo re qapa lethathamo la tharollo ea rona ka linako tsohle ho khotsofatsa litlhoko tse ncha le ho latela nts'etsopele ea morao-rao ea 'maraka oa Malta.Re itokiselitse ho tobana le matšoenyeho le ho ntlafatsa ho utloisisa menyetla eohle ea khoebo ea machaba.

Litlhaloso

50 Preps, bakeng sa tlhoekiso ea DNA ea genomic ho tsoa mali a felletseng a sebelisa mali a <1ml

Buffer DC: E hlahisa lisele tse khubelu tsa mali maling.

Buffer BL1: Fana ka mohlala oa tikoloho ea tšilo ea enzymatic.

Foregene Protease Plus: Mesampole ea mali e sila ka Enzymatic tikolohong ea Buffer BL1.

Buffer BL2: Etsa hore Foregene Protease Plus e sebetse 'me u fane ka tikoloho ea ho kenya DNA.

Buffer PW: Tlosa litšila tse kang protheine le RNA ho DNA.

Buffer WB1: Tlosa li-ion tse setseng tsa letsoai ho DNA.

Buffer EB: Elute DNA ka lera la tlhoekiso.

DNA-Feela Kholomo: Ka ho khetheha e adsorbs genomic DNA ka lysate.

Likarolo tsa Kit

Likarolo le melemo

-Ha ho tšilafalo ea RNase: Kholomo ea DNA-Feela e fanoeng ke kit e etsa hore ho khonehe ho tlosa RNA ho DNA ea genomic ntle le ho eketsa RNase nakong ea teko, ho qoba laboratori hore e se ke ea silafatsoa ke RNase e sa tloaelehang.

-Lebelo le potlakileng: Foregene Protease Plus e na le ts'ebetso e phahameng ho feta li-protease tse tšoanang, 'me e sila lisampole tsa linama kapele;ts'ebetso e bonolo, 'me ts'ebetso ea ho ntša DNA ea genomic e ka phethoa nakong ea metsotso e 40.

-Bonolo: The centrifugation e etsoa mocheso oa kamore, 4 ° C centrifugation kapa ethanol precipitation ea DNA ha e hlokehe.

-Tshireletso: Ha ho qotsulo ea organic reagent e sebelisoang.

-Boleng bo phahameng: Likaroloana tse nkiloeng tsa genomic DNA li kholo, ha ho na RNA, ha ho RNase, le li-ion tse tlaase haholo, tse ka finyellang litlhoko tsa liteko tse fapa-fapaneng.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisa DNA ea genomic maling a foreshe kapa a leqhoa a anticoagulated.

Mokhoa oa ho sebetsa

Storage le Shelf bophelo

Setsi sena se ka bolokoa likhoeli tse 12 tlas'a maemo a omileng mocheso oa kamore (15-25 ° C);haeba e hloka ho bolokoa nako e telele, e ka bolokoa ho 2-8°C.

Tlhokomeliso: Haeba e bolokiloe mochesong o tlase, tharollo e atisa ho ba le pula.Pele o sebelisoa, etsa bonnete ba hore o beha tharollo ka har'a kit mocheso oa kamore ka nako e itseng.Haeba ho hlokahala, e chese pele ka ho hlapa metsi a 37 ° C ka metsotso e 10 ho qhaqha precipitate, 'me e kopanye pele e sebelisoa.

Tharollo ea Foregene Protease Plus e na le foromo e ikhethang, e sebetsang ha e bolokiloe mocheso oa kamore nako e telele (likhoeli tse 3);e tla ba e sebetsang haholoanyane le e tsitsitseng ha e bolokiloe ka 4 ° C, kahoo ho kgothaletswa ho e boloka ho 4 ° C, hopola hore u se ke ua e boloka ho -20 ° C. Re lumela hore tšebelisano ea nako e telele ea polelo e hlile e bakoa ke boleng ba boleng bo phahameng, litšebeletso tse ekelitsoeng, phihlelo e atlehileng le ho ikopanya le motho ka mong bakeng sa Fektheri ea Virus Magnetic Bead Method Rna-DNA Nucleic Acid , Reciation ea Nucleic Acid 96 hantle-Tsela ea ho phunya le ho qhekella ho sebetsa hantle. matla a ka theha likamano tse ntle tsa khoebo le uena kapele.Etsa bonnete ba hore o ikutloa o lokolohile ka botlalo ho ikopanya le rona bakeng sa lintlha tse ling.
lifeme tsa mabenkele bakeng saChina Nucleic Acid Extraction Kit (rna-DNA) le 96 Nucleic Acid, Ka ho tsitlallela taolong ea boleng ba boleng bo holimo le thuso ea bareki, joale re thehile qeto ea rona ea ho fa bareki ba rona ho qala ka ho fumana chelete le ka mor'a boiphihlelo ba lits'ebeletso.Ho boloka likamano tsa botsoalle tse teng le bareki ba rona, leha ho le joalo re qapa lethathamo la tharollo ea rona ka linako tsohle ho khotsofatsa litlhoko tse ncha le ho latela nts'etsopele ea morao-rao ea 'maraka oa Malta.Re itokiselitse ho tobana le matšoenyeho le ho ntlafatsa ho utloisisa menyetla eohle ea khoebo ea machaba.