Re lumela ho: Boqapi ke moea le moea oa rona.Boemo bo holimo ke bophelo ba rona.Tlhokahalo ea bareki ke Molimo oa rona bakeng sa mohloli oa fektheri oa China CE/FDA Mokhoa o Amoheletsoeng oa Lifaha tsa Magnetic DNA / Rna Nucleic Acid Extraction Kits (Lifaha tsa Magnetic), Etsa bonnete ba hore u na le boiphihlelo ba mahala ho ikopanya le rona neng kapa neng.Re tla u araba ha re fumana lipotso tsa hau.Ka kopo hlokomela hore mehlala e teng pele re qala khoebo ea rona.
Re lumela ho: Boqapi ke moea le moea oa rona.Boemo bo holimo ke bophelo ba rona.Moreki tlhoko ke Molimo oa rona bakeng saChina Nucleic Acid, China Nucleic Acid Manufacturers, Ntle le matla a matla a tekheniki, re boetse re kenyelletsa lisebelisoa tse tsoetseng pele bakeng sa tlhahlobo le ho tsamaisa taolo e thata.Basebetsi bohle ba k'hamphani ea rona ba amohela metsoalle ea lapeng le kantle ho naha hore e tle bakeng sa maeto le khoebo motheong oa tekano le melemo e kopaneng.Haeba u thahasella efe kapa efe ea lintho tsa rona, hopola ho ikutloa u lokolohile ho ikopanya le rona bakeng sa khotheishene le lintlha tsa sehlahisoa.

Litlhaloso tsa Kits

50 Preps, 200 Preps

Setsi sena se sebelisa "spin column le formula" e entsoeng ke k'hamphani ea rona, e ka ntšang RNA e hloekileng le ea boleng bo phahameng ka ho fetisisa ho tsoa ho mefuta e sa tšoaneng ea liphoofolo tse nang le bokhoni bo phahameng.E fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo le ho bapatsa DNA ea genomic ho tloha ho supernatant le lysate ea lisele, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.

Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, ​​e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.

Likarolo tsa Kit:

Likarolo le melemo

■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.

Lihlahisoa tsa lihlahisoa

■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×10⁶
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl

Phallo ea mosebetsi

Setšoantšo

Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar

Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer RL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli ea 1 ka mor'a ho eketsa β- mercaptoethanol (ho ikhethela).

Re lumela ho: Boqapi ke moea le moea oa rona.Boemo bo holimo ke bophelo ba rona.Tlhokahalo ea bareki ke Molimo oa rona bakeng sa mohloli oa fektheri oa China CE/FDA Mokhoa o Amoheletsoeng oa Lifaha tsa Magnetic DNA / Rna Nucleic Acid Extraction Kits (Lifaha tsa Magnetic), Etsa bonnete ba hore u na le boiphihlelo ba mahala ho ikopanya le rona neng kapa neng.Re tla u araba ha re fumana lipotso tsa hau.Ka kopo hlokomela hore mehlala e teng pele re qala khoebo ea rona.
Mohloli oa fektheriChina Nucleic Acid, China Nucleic Acid Manufacturers, Ntle le matla a matla a tekheniki, re boetse re kenyelletsa lisebelisoa tse tsoetseng pele bakeng sa tlhahlobo le ho tsamaisa taolo e thata.Basebetsi bohle ba k'hamphani ea rona ba amohela metsoalle ea lapeng le kantle ho naha hore e tle bakeng sa maeto le khoebo motheong oa tekano le melemo e kopaneng.Haeba u thahasella efe kapa efe ea lintho tsa rona, hopola ho ikutloa u lokolohile ho ikopanya le rona bakeng sa khotheishene le lintlha tsa sehlahisoa.