Re khona ho khotsofatsa bareki ba rona ka kakaretso ka boleng ba rona bo botle haholo, theko e holimo le tšehetso e ntle ka lebaka la hore re bile litsebi tse ngata le ho sebetsa ka thata mme re e etsa ka mokhoa o theko e tlase bakeng sa thekiso ea thepa ea fektheri ea China Plastic Magnetic Bead Method Universal Nucleic Acid Extraction Rapid Kit, Lihlahisoa tsa rona li hlahlojoa ka thata pele re fumana thepa e ntle lefatšeng ka bophara.Re labalabela ho sebelisana le uena nakong e tlang.
Ka kakaretso re khona ho khotsofatsa bareki ba rona ba hlomphuoang ka boleng ba rona bo botle haholo, theko e holimo le tšehetso e ntle ka lebaka la hore re bile litsebi tse ngata le ho sebetsa ka thata ho feta, 'me re e etsa ka mokhoa o sa reroang.China Universal Nucleic Acid Extraction, Plastic Nucleic Acid Extraction, Khamphani ea rona e latela mohopolo oa botsamaisi oa "boloka boqapi, phehella bokhabane".Motheong oa ho netefatsa melemo ea litharollo tse teng, re tsoela pele ho matlafatsa le ho atolosa nts'etsopele ea lihlahisoa.Khamphani ea rona e tsitlallela ho nchafatsoa ho nts'etsapele nts'etsopele e tsitsitseng ea likhoebo, le ho re etsa hore re be bafani ba thepa ea lapeng ba boleng bo holimo.

Litlhaloso

50 Preps, 200 Preps

Thepa e sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo ho lithane tse fapaneng tsa semela tse nang le li-polysaccharides tse tlase le polyphenols.Bakeng sa lisampole tsa semela tse nang le li-polysaccharides tse phahameng kapa polyphenols, ho khothaletsoa ho sebelisa Plant Total RNA Isolation Plus Kit ho fumana liphetho tse ntle tsa ho ntšoa ha RNA.Kit e fana ka kholomo ea DNA-Cleaning e ka tlosang DNA ea genomic habonolo ho supernatant le lysate ea lisele.Kholomo ea RNA feela e ka tlama RNA ka nepo.Kit e ka sebetsana le palo e kholo ea lisampole ka nako e le 'ngoe.

Tsamaiso eohle ha e na RNase, kahoo RNA e hloekisitsoeng e ke ke ea senyeha.Buffer PRW1 le Buffer PRW2 e ka etsa bonnete ba hore RNA e fumanoeng ha e silafatsoe ke liprotheine, DNA, ions, le metsoako ea tlhaho.

Likarolo tsa Kit

Likarolo le melemo

■ Ts'ebetso ka mocheso oa kamore (15-25 ℃) ho pholletsa le ts'ebetso eohle, ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Complete Kit RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama ho DNA, e le hore kit e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa nakong ea metsotso e 30.
■ Tšireletseho: ha ho na reagent ea tlhaho e hlokahalang.
■ Boleng bo phahameng: Likaroloana tsa RNA tse hloekisitsoeng li hloekile haholo, ha li na protheine le litšila tse ling, 'me li ka kopana le mekhoa e fapaneng ea liteko tse tlase.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha kakaretso ea RNA ho tloha lisampoleng tse foreshe kapa tse hatselitsoeng (haholo-holo mahlaku a mahlaku a semela) a nang le polysaccharide e tlase le polyphenol.

Phallo ea mosebetsi

Setšoantšo

Plant Total RNA Isolation Kit Plus e sebelitse 50mg ea makhasi a macha a polysaccharides le polyphenols, 'me 5% e hloekisitsoeng ea RNA e ile ea lekoa ke electrophoresis.
1: Banana
2: Ginkgo
3: K'hothone
4: Kharenate

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) sebakeng se omileng, le 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer PSL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(khetho).

Re khona ho khotsofatsa bareki ba rona ka kakaretso ka boleng ba rona bo botle haholo, theko e holimo le tšehetso e ntle ka lebaka la hore re bile litsebi tse ngata le ho sebetsa ka thata mme re e etsa ka mokhoa o theko e tlase bakeng sa thekiso ea thepa ea fektheri ea China Plastic Magnetic Bead Method Universal Nucleic Acid Extraction Rapid Kit, Lihlahisoa tsa rona li hlahlojoa ka thata pele re fumana thepa e ntle lefatšeng ka bophara.Re labalabela ho sebelisana le uena nakong e tlang.
Lifeme tse rekisoang ka bongataChina Universal Nucleic Acid Extraction, Plastic Nucleic Acid Extraction, Khamphani ea rona e latela mohopolo oa botsamaisi oa "boloka boqapi, phehella bokhabane".Motheong oa ho netefatsa melemo ea litharollo tse teng, re tsoela pele ho matlafatsa le ho atolosa nts'etsopele ea lihlahisoa.Khamphani ea rona e tsitlallela ho nchafatsoa ho nts'etsapele nts'etsopele e tsitsitseng ea likhoebo, le ho re etsa hore re be bafani ba thepa ea lapeng ba boleng bo holimo.