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Fast Nucleic Acid Extraction Kit ea Microbial ka Lijo

Tlhaloso ea Kit:

Katse.No.TK101

 

 

Setsi sena se nka mokhoa o ikhethileng oa tharollo, o bonolo ebile o bonolo ntle le ho ntšoa le ho hloekisoa, 'me o ka sebelisoa ka kotloloho bakeng sa ho lemoha ha nucleic acid amplification.

matla a foregene


Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

LBH

TS'ELISITSOE MOHLOLI

Litlhaloso

Setsi sena se nka mokhoa o ikhethileng oa tharollo, o bonolo ebile o bonolo ntle le ho ntšoa le ho hloekisoa, 'me o ka sebelisoa ka kotloloho bakeng sa ho lemoha ha nucleic acid amplification.

Litaba tsa Kit

Lebitso

Motsoako

Tlhaloso

Moemeli oa Phallo ea Asiti ea Nucleic

Lysate

6mL

Tšebeliso e Lebeletsoeng

E sebelisetsoa ho ntša nucleic acid ka potlako ho tsoa lijong.

Maemo a polokelo le Letsatsi la ho Felloa ke Nako

Boloka mocheso oa kamore, o sebetsa likhoeli tse 12.

Lisebelisoa le Lisebelisoa

Bate ea metsi kapa bate ea tšepe, centrifuge ea lebelo le holimo (e sebelisoang bakeng sa sampole ea kalafo ea pele), pipette, le malebela.

Tšebeliso

1.MohlalaPre-phekolo

Tšoara mohlala Sheba GB4789 kapa maemo a mang a indasteri.

2.NHo tlosoa ha ucleic acid

Nka 20 ul ea tharollo ea ho ntlafatsa ka har'a lysate tube, vortex bakeng sa metsotsoana ea 30, centrifuge ka bokhutšoanyane, 'me u behelle ka thōko.

 Tlhaloso: Ho ntšoa ha nucleic acid ho tloha lysate ho lokela ho phethoa ka hare ho metsotso ea 10 'me e ke ke ea bolokoa nako e telele.

Notice

1. Ho khothalletsoa ho apara liaparo tse hloekileng tsa mosebetsi le liatlana nakong ea teko.Malebela a sebelisitsoeng a lokela ho hloekisoa esale pele, 'me litšila tsohle tse hlahisitsoeng tekong li lokela ho lahloa ka nako.

2. Ka kopo latela ka tieo mehato ea ts'ebetso.

3. Ka kopo sebelisa khiti nakong ea nako ea ho sebetsa.


  • E fetileng:
  • E 'ngoe:

  • Litaelo tsa tlhahlobo ea mathata

    The following is an analysis of the problems that might be encountered in the extraction of viral RNA. We wish it would be helpful to your experiment. In addition, for other experimental or technical problems other than operating instructions and problem analysis, we have dedicated technical support to help you. Contact us if you need at : 028-83361257or E-mail:Tech@foregene.com。

     

    Ha ho RNA e ka ntšoang kapa chai ea nucleic acid e tlase

    Hangata ho na le lintlha tse ngata tse amang katleho ea ho hlaphoheloa, joalo ka: sampole ea litaba tsa RNA, mokhoa oa ts'ebetso, bophahamo ba molumo, joalo-joalo.

    Tlhahlobo ea lisosa tse tloaelehileng:

    1.Bate ea leqhoa kapa mocheso o tlase (4 ° C) centrifugation nakong ea ts'ebetso.

    Tlhahiso: Mocheso oa kamore (15-25 ° C) o sebetsa, ha ho mohla ho hlatsoa leqhoa le mocheso o tlase oa centrifuge.

    2. Polokelo e sa nepahalang ea mohlala kapa polokelo ea mohlala nako e telele haholo.

    Tlhahiso: Boloka lisampole ho -80 ° C kapa u hatsele ka har'a metsi a naetrojene, 'me u qobe tšebeliso ea ho qhibiliha khafetsa;leka ho sebelisa lisampole tse sa tsoa bokelloa bakeng sa ho ntša RNA.

    3.Sample e sa lekaneng ea lysis

    Keletso: Ka kopo etsa bonnete ba hore sampole le tharollo e sebetsang (Linear Acrylamide) li tsoakoe ka botlalo 'me li kentsoe metsotso e 10 mocheso oa kamore (15-25 ° C)

    4.The eluent e kentsoe ka phoso

    Keletso: Etsa bonnete ba hore RNase-Free ddH2O e kentsoe bohareng ba lera la kholomo ea tlhoekiso.

    5.Molumo o sa nepahalang oa ethanol ea anhydrous ho Buffer viRW2

    Tlhahiso: Ka kopo latela litaelo, eketsa molumo o nepahetseng oa ethanol ea anhydrous ho Buffer viRW2 'me u li kopanye hantle pele u sebelisa kit.

    6.Tsebeliso e fosahetseng ea sampole.

    Tlhahiso: 200µl ea sampole ka 500μl ea Buffer viRL.Bophahamo bo feteletseng ba sampole bo tla fella ka ho fokotseha ha sekhahla sa ho ntšoa ha RNA.

    7.Molumo o sa nepahalang oa tlhahiso kapa tlhahiso e sa fellang.

    Tlhahiso: Molumo o hlakileng oa kholomo ea tlhoekiso ke 30-50μl;haeba phello ea elution e sa khotsofatse, ho kgothaletswa ho eketsa RNase-Free ddH e futhumetseng2O le ho eketsa nako ea ho beha mocheso oa kamore, joalo ka 5-10min

    8.Kolumo ea tlhoekiso e na le masalla a ethanol ka mor'a ho hlatsoa Buffer viRW2.

    Tlhahiso: Haeba ethanol e ntse e sala ka mor'a hore e hlatsoe ka Buffer viRW2 le centrifugation e se nang letho bakeng sa 2min, kholomo ea tlhoekiso e ka siuoa mocheso oa kamore bakeng sa 5min ka mor'a hore ho be le letho-tube centrifugation ho tlosa ethanol e setseng ka botlalo.

     

    Ho senyeha ha limolek'hule tsa RNA tse hloekisitsoeng

    Boleng ba RNA e hloekisitsoeng bo amana le lintlha tse kang polokelo ea sampuli, tšilafalo ea RNase, le ts'ebetso.

    Tlhahlobo ea lisosa tse tloaelehileng:

    1.Lisampole tse bokeletsoeng ha lia ka tsa bolokoa ka nako.

    Tlhahiso: Haeba sampole e sa sebelisoe ka nako kamora ho bokelloa, ka kopo e boloke ho -80 ℃ kapa metsi a naetrojene hang-hang.Bakeng sa ho ntša limolek'hule tsa RNA, leka ho sebelisa lisampole tse sa tsoa bokelloa neng kapa neng ha ho khoneha.

    2.Collected disampole ba ne ba hoama le ho qhibiliha khafetsa.

    Tlhahiso: Qoba ho qhoqhoa khafetsa le ho qhibiliha (e seng ho feta hanngoe) nakong ea ho bokella le ho boloka sampole, ho seng joalo chai ea nucleic acid e tla fokotseha.

    3.RNase e ile ea hlahisoa ka kamoreng ea ho buuoa kapa ho ne ho se na liatlana tse lahliloeng, limaske, joalo-joalo.

    Tlhahiso: Ho ntšoa ha liteko tsa limolek'hule tsa RNA ho etsoa hantle ka phaposing e arohaneng ea tšebetso ea RNA, 'me tafole ea liteko e hloekisoa pele ho teko.Apara liatlana le limaske tse lahloang nakong ea liteko ho qoba ho senyeha ha RNA ho bakoang ke kenyelletso ea RNase.

    4.The reagent e silafalitsoe ke RNase nakong ea tšebeliso.

    Tlhahiso: Kenya sebaka ka Viral RNA Isolation Kit e ncha bakeng sa liteko tse amanang.

    5. Tšilafalo ea RNase ea liphaephe tsa centrifuge, malebela a pipette, joalo-joalo. Tlhahiso: Etsa bonnete ba hore li-tubes tsa centrifuge, malebela a pipette, le lipeipi kaofela ha li na RNase.

     

    Limolek'hule tsa RNA tse hloekisitsoeng li ile tsa ama liteko tse tlase

    Limolek'hule tsa RNA tse hloekisitsoeng ke kholomo ea tlhoekiso li tla ama liteko tse tlase tsa metsi haeba ho na le li-ion tsa letsoai kapa liprotheine tse ngata haholo, joalo ka: reverse transcription, Northern Blot, joalo-joalo.

    1.Ho na le li-ion tse setseng tsa letsoai ka har'a limolek'hule tsa eluted RNA.

    Keletso: Etsa bonnete ba hore molumo o nepahetseng oa ethanol ea anhydrous e kentsoe Buffer viRW2, 'me u hlatsoe kholomo ea tlhoekiso habeli ho latela lebelo le nepahetseng la centrifugation litaelong tsa ts'ebetso; Haeba ho ntse ho e-na le li-ion tsa letsoai tse setseng, u ka eketsa Buffer viRW2 kholomong ea tlhoekiso, 'me u e tlohele mocheso oa kamore bakeng sa 5min.Ebe u etsa centrifugation ho tlosa silafatso ea ion ea letsoai ka tekanyo e kholo ka ho fetisisa

    2.Ho na le ethanol e setseng ka har'a limolek'hule tsa RNA

    Tlhahiso: hang ha o netefatsa hore litšiea tsa tlhoekiso li hlatsoitsoe ke Buffer viRW2, etsa centrifugation e se nang letho ho latela lebelo la centrifugal ho litaelo tsa ts'ebetso.Haeba ho ntse ho e-na le ethanol e setseng, e ka tloheloa metsotso e 5 mocheso oa kamore ka mor'a hore ho be le centrifugation e se nang letho ho tlosa ethanol e setseng ka tekanyo e kholo ka ho fetisisa.

    Litaelo:

    Viral RNA Isolation Kit Instruction Manual

     

    Ngola molaetsa wa hao mona mme o re romele wona