Mehlala e Ntle ea Phatlalatso ea Release Reagent Nucleic Acid Extraction-Free Vtm Kit Mohato o le Mong oa Tlhahlobo oa PCR
Tlhaloso ea Kit:
Mohale oa 2 × PCRTM Sistimi ea Mix e na le mamello e phahameng ho li-inhibitors tsa PCR ho feta sistimi e tloaelehileng ea PCR Mix, 'me e khona ho sebetsana habonolo le ntlafatso ea PCR ea litempele tse fapaneng tse rarahaneng.Sistimi e ikhethang ea karabelo le ts'ebetso e phahameng ea Taq Hero li etsa hore karabelo ea PCR e be le bokhoni bo phahameng ba ho holisa, boits'oaro le kutlo.
E inehetse ho taolo e thata ea boleng le litšebeletso tse nahanelang tsa bareki, basebetsi ba rona ba nang le boiphihlelo ba lula ba le teng ho buisana ka litlhoko tsa hau le ho netefatsa khotsofalo e felletseng ea bareki bakeng sa Good Quality Sample Release Reagent Nucleic Acid Extraction-Free Vtm Kit One Step PCR Test Method, Etsa bonnete ba hore u ikutloa u se na litšenyehelo tsa ho bua le rona bakeng sa mokhatlo.mme re nahana hore re tla arolelana boiphihlelo bo nepahetseng ba khoebo le barekisi bohle ba rona.
E inehetse ho taolo e tiileng ea boleng le tšebeletso e nahanang ea bareki, basebetsi ba rona ba nang le boiphihlelo ba lula ba le teng ho buisana ka litlhoko tsa hau le ho netefatsa khotsofalo e felletseng ea bareki bakeng saChina Sample Release Kit le Mohato o le Mong, Re na le sehlopha sa barekisi se inehetseng le se mabifi, le makala a mangata, a sebeletsang bareki ba rona.Re batla litšebelisano tsa nako e telele tsa khoebo, 'me re netefatsa hore barekisi ba rona ba tla rua molemo ka botlalo nakong e khutšoane le e telele.
Litlhaloso
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Likarolo tsa Kit
| Karolo ea I | Buffer P1 |
| Buffer P2 | |
| 6×DNA Loading Buffer | |
| Karolo ea II | 2 × Lekhasi la PCR le BonoloTMMotsoako(UNG) |
Likarolo le melemo
■ Ha ho tlhoekiso ea DNA e jang nako le e theko e boima
■ Boitsebiso bo fokolang
■ E bonolo-sampole e ka ba tlas'a PCR reaction kapa lysis reaction ntle le ho seha kapa ho sila
■ 2 × Mix, ho fokotsa liphoso tsa ho kenya sampole le nako ea ho lokisa sistimi
■ Tokisetso e potlakileng ea template e ka phethoa ka metsotso e 10, karabelo ea PCR e ka phethoa bonyane ka metsotso e 50.
■ Boitšoaro bo phahameng ba throughput-lysis bo ka phethoa ka poleiti ea PCR ea seliba sa 96
Litekanyetso tsa kit
Kopo: boitsebiso bo fetotsoeng ka liphatsa tsa lefutso, genotyping, joalo-joalo.
Mohlala: lekhasi la semela
Tekanyo: Diameter 2-3mm (mokhoa o tobileng), bophara ba 5-7mm (mokhoa oa lysis)
Sebaka sa ho lemoha: sekhechana sa sepheo ≤1kb
Phallo ea mosebetsi
Electropherogram
Libeke tse peli tsa liphetho tsa ho lemoha ha PCR Mix tlas'a boemo ba tšilafalo ea sehlahisoa sa PCR. E inehetse ho laola boleng bo thata le tšebeletso e nahanang ea bareki, basebetsi ba rona ba nang le phihlelo ba lula ba le teng ho buisana ka litlhoko tsa hau le ho netefatsa khotsofalo e feletseng ea bareki bakeng sa Good Quality Sample Release Reagent Nucleic Acid Extraction-Free Vtm Kit One Step PCR Test Method, Etsa bonnete ba hore u tla ho bua le rona ntle le litšenyehelo.mme re nahana hore re tla arolelana boiphihlelo bo nepahetseng ba khoebo le barekisi bohle ba rona.
Boleng bo Botle ba China Samle Release Kit le One Step, Re na le sehlopha sa barekisi se inehetseng le se mabifi, le makala a mangata, a sebeletsang bareki ba rona.Re batla litšebelisano tsa nako e telele tsa khoebo, 'me re netefatsa hore barekisi ba rona ba tla rua molemo ka botlalo nakong e khutšoane le e telele.
