Setumo se Ntle sa Mosebelisi sa China Nucleic Acid Extraction Kit bakeng sa Tlhahlobo ea DNA ea Mali ea Nucleic Acid Purification Reagent.
Re natefeloa ke botumo bo botle bo makatsang har'a bareki ba rona bakeng sa thepa ea rona ea boleng bo holimo, lebelo le mabifi hammoho le ts'ehetso e sebetsang ka ho fetesisa bakeng sa Setumo se Ntle sa Mosebelisi bakeng sa China Nucleic Acid Extraction Kit bakeng sa Tlhahlobo ea DNA ea Mali ea Nucleic Acid Purification Reagent, Litebello pele!Eng kapa eng eo u e hlokang, re lokela ho etsa sohle se matleng a rona ho u thusa.Re amohela ka mofuthu bareki ba tsoang lefats'eng lohle ho sebelisana le rona molemong oa ntlafatso.
Re natefeloa ke botumo bo botle ka tsela e makatsang har'a bareki ba rona bakeng sa thepa ea rona ea boleng bo holimo, lebelo le mabifi hammoho le ts'ehetso e sebetsang hoChina Nucleic Acid Extraction Reagent, Mokhoa oa Magnetic Bead, Re na le baenjiniere ba hloahloa liindastering tsena le sehlopha se sebetsang hantle lipatlisisong.Ho feta moo, joale re na le melomo le mebaraka ea rona ea polokelo ea litaba Chaena ka theko e tlase.Ka hona, re ka kopana le lipotso tse fapaneng ho tsoa ho bareki ba fapaneng.Hopola ho fumana sebaka sa rona sa marang-rang ho sheba lintlha tse ling ho tsoa linthong tsa rona.
Litlhaloso tsa Kits
50 Preps, 200 Preps
Setsi sena se sebelisa "spin column le formula" e entsoeng ke k'hamphani ea rona, e ka ntšang RNA e hloekileng le ea boleng bo phahameng ka ho fetisisa ho tsoa ho mefuta e sa tšoaneng ea liphoofolo tse nang le bokhoni bo phahameng.E fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo le ho bapatsa DNA ea genomic ho tloha ho supernatant le lysate ea lisele, e bonolo le e bolokang nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.
Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.
Likarolo tsa Kit:
| Buffer RL1 , Buffer RL2 |
| Buffer RW1, Buffer RW2 |
| RNase-Free ddH2O, DNA-Cleaning Column |
| RNA-Feela Kholomo |
Likarolo le melemo
■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1
Sesebelisoa sa Kit
E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.
Lihlahisoa tsa lihlahisoa
■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×106
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl
Phallo ea mosebetsi
Setšoantšo
Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar
Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo
Polokelo le shelf bophelo
Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer RL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli ea 1 ka mor'a ho eketsa β- mercaptoethanol (ho ikhethela).
Re natefeloa ke botumo bo botle bo makatsang har'a bareki ba rona bakeng sa thepa ea rona ea boleng bo holimo, lebelo le mabifi hammoho le ts'ehetso e sebetsang ka ho fetesisa bakeng sa Setumo se Ntle sa Mosebelisi bakeng sa China Nucleic Acid Extraction Kit bakeng sa Tlhahlobo ea DNA ea Mali ea Nucleic Acid Purification Reagent, Litebello pele!Eng kapa eng eo u e hlokang, re lokela ho etsa sohle se matleng a rona ho u thusa.Re amohela ka mofuthu bareki ba tsoang lefats'eng lohle ho sebelisana le rona molemong oa ntlafatso.
Setumo se setle sa mosebelisi bakeng saChina Nucleic Acid Extraction Reagent, Mokhoa oa Magnetic Bead, Re na le baenjiniere ba hloahloa liindastering tsena le sehlopha se sebetsang hantle lipatlisisong.Ho feta moo, joale re na le melomo le mebaraka ea rona ea polokelo ea litaba Chaena ka theko e tlase.Ka hona, re ka kopana le lipotso tse fapaneng ho tsoa ho bareki ba fapaneng.Hopola ho fumana sebaka sa rona sa marang-rang ho sheba lintlha tse ling ho tsoa linthong tsa rona.
