Feme ea rona e ts'episa basebelisi bohle ba lihlahisoa le litharollo tsa maemo a pele hammoho le thuso e khotsofatsang ka ho fetesisa ea kamora thekiso.Re amohela ka mofuthu bareki ba rona ba kamehla le ba bacha hore ba ikopanye le rona bakeng sa High Quality China Rna Purification Kit (Spin Column), Re sebelisa sepheo sa ka ho sa feleng sa "ntlafatso ea boleng bo tsoelang pele, khotsofalo ea bareki", re na le bonnete ba hore thepa ea rona e ntle e bolokehile ebile e na le boikarabello 'me lihlahisoa tsa rona le litharollo li rekisoa haholo lapeng la hau le mose ho maoatle.
Feme ea rona e ts'episa basebelisi bohle ba lihlahisoa le litharollo tsa maemo a pele hammoho le thuso e khotsofatsang ka ho fetesisa ea kamora thekiso.Re amohela ka mofuthu bareki ba rona ba kamehla le ba bacha ho ikopanya le rona bakeng saChina Rna Extraction, Dna Test, Amohela lipotso leha e le life tsa hau le lintho tse u tšoenyang ka thepa ea rona.Re labalabela ho theha likamano tsa nako e telele tsa khoebo le uena haufinyane.Ikopanye le rona kajeno.Re molekane oa hau oa pele oa khoebo!

Tlhaloso

50 Preps, 200 Preps

Setsi sena se sebelisa "spin column le formula" e ntlafalitsoeng ke Foregene, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo liseleng tse entsoeng ka lipoleiti tse 96, 24, 12 le 6.

Thepa e fana ka DNA-Cleaning Column e sebetsang hantle, e ka arolang habonolo supernatant le cell lysate, ho tlama le ho tlosa DNA ea genomic.Opereishene e bonolo ebile e boloka nako.

Kholomo ea RNA-feela e ka tlama RNA hantle ka foromo e ikhethang.Palo e kholo ea disampole e ka sebetswa ka nako e le nngwe.

Likarolo tsa Kit

*Ka kopo, roala liatlana 'me u nke mehato ea ts'ireletso nakong ea ts'ebetso kaha Buffer cRL1 le Buffer RW1 li na le matsoai a chaotropic a halefisang.

Likarolo le melemo

■ Tshebetso eohle e sebetsa mocheso oa kamore (15-25 ℃), ntle le ho hlatsoa leqhoa le mocheso o tlaase oa centrifugation.
■ Thepa eohle ha e na RNase-Free, ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA.
■ DNA-Cleaning Column ka ho khetheha e tlama DNA, e le hore khiti e ka tlosa tšilafalo ea DNA ea genomic ntle le ho eketsa DNase e eketsehileng.
■ Poelo e phahameng ea RNA: Kholomo ea RNA feela le foromo e ikhethang e ka hloekisa RNA hantle.
■ Lebelo le potlakileng: ho bonolo ho sebetsa mme le ka phethoa ka metsotso e 11.
■ Polokeho: Ha ho hlokahale reagent ea tlhaho.
■ Boleng bo phahameng: RNA e hloekisitsoeng e hloekile haholo, ha e na protheine le litšila tse ling, ’me e ka kopana le liteko tse sa tšoaneng tse latelang.

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa liseleng tse hlahisitsoeng ka har'a lipoleiti tse 96, 24, 12 le 6 tsa seliba.

Phallo ea mosebetsi

Setšoantšo

Sets'oants'o sa betri ea gel ea agarose ea Cell Total RNA Isolation Kit se tšoere linomoro tse kaholimo tse fapaneng tsa lisele, 20μl bophahamo ba modumo, nka 2μl e hloekisitsoeng RNA 1%.

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 12 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele (likhoeli tse 24).

Buffer cRL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli e le 'ngoe ka mor'a ho eketsa 2-hydroxy-1-ethanethiol(khetho).

Feme ea rona e ts'episa basebelisi bohle ba lihlahisoa le litharollo tsa maemo a pele hammoho le thuso e khotsofatsang ka ho fetesisa ea kamora thekiso.Re amohela ka mofuthu bareki ba rona ba kamehla le ba bacha hore ba ikopanye le rona bakeng sa High Quality China Rna Purification Kit (Spin Column), Re sebelisa sepheo sa ka ho sa feleng sa "ntlafatso ea boleng bo tsoelang pele, khotsofalo ea bareki", re na le bonnete ba hore thepa ea rona e ntle e bolokehile ebile e na le boikarabello 'me lihlahisoa tsa rona le litharollo li rekisoa haholo lapeng la hau le mose ho maoatle.
Boleng bo phahamengChina Rna Extraction, Teko ea DNA, Amohela lipotso leha e le life tsa hau le lintho tse u tšoenyang ka thepa ea rona.Re labalabela ho theha likamano tsa nako e telele tsa khoebo le uena haufinyane.Ikopanye le rona kajeno.Re molekane oa hau oa pele oa khoebo!